P0A1W2 (LEP_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal peptidase I
Short name=SPase I
EC=3.4.21.89

Alternative name(s):
Leader peptidase I

Gene names

Name:	lepB
Ordered Locus Names:	STM2582

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Signal peptidase I		PRO_0000109518
Regions
	Topological domain	1 – 3	3	Periplasmic Potential
	Transmembrane	4 – 22	19	Helical; Potential
	Topological domain	23 – 58	36	Cytoplasmic Potential
	Transmembrane	59 – 77	19	Helical; Potential
	Topological domain	78 – 324	247	Periplasmic Potential
Sites
	Active site	91	1	By similarity
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A1W2 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 40607CDA15779575	FASTA	324	35,778
	10 20 30 40 50 60 MANMFALILV IATLVTGILW CVDKFVFAPK RRARQAAAQT ASGDALDNAT LNKVAPKPGW 70 80 90 100 110 120 LETGASVFPV LAIVLIVRSF LYEPFQIPSG SMMPTLLIGD FILVEKFAYG IKDPIYQKTL 130 140 150 160 170 180 IETGHPKRGD IVVFKYPEDP KLDYIKRAVG LPGDKITYDP VAKEVTIQPG CSSGQACENA 190 200 210 220 230 240 LPVTYSNVEP SDFVQTFARR NGGEATSGFF EVPLNETKEN GIRLTERKET LGDVTHRILM 250 260 270 280 290 300 VPIAQDQLGM YYQQPGQPLA TWVVPPGQYF MMGDNRDNSA DSRYWGFVPE ANLVGKAVAI 310 320 WMSFDKQEGE WPTGVRLSRI GGIH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the Salmonella typhimurium lep gene in Escherichia coli." van Dijl J.M., van den Bergh R., Reversma T., Smith H., Bron S., Venema G. Mol. Gen. Genet. 223:233-240(1990) [PubMed: 2250650] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PP1139.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54933 Genomic DNA. Translation: CAA38694.1. AE006468 Genomic DNA. Translation: AAL21476.1.
PIR	S12020.
RefSeq	NP_461517.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P0A1W2.
SMR	P0A1W2. Positions 78-324.
ModBase	Search...
Protein family/group databases
MEROPS	S26.001.
Proteomic databases
PRIDE	P0A1W2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1254104.
GenomeReviews	Gene locus STM2582 in contig AE006468_GR.
KEGG	stm:STM2582.
PATRIC	32383801. VBISalEnt20916_2723.
Phylogenomic databases
HOGENOM	HBG596607.
OMA	PTGVRFE.
ProtClustDB	PRK10861.
Enzyme and pathway databases
BioCyc	STYP99287:STM2582-MONOMER.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR019533. Peptidase_S26. IPR019766. Peptidase_S26A_all-beta_subdom. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 2 hits. G3DSA:2.170.230.10. Peptidase_S26A_all-beta_subdom. 1 hit.
KO	K03100.
Pfam	PF00717. Peptidase_S24. 1 hit. PF10502. Peptidase_S26. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR02227. Sigpep_I_bact. 2 hits.
PROSITE	PS00501. SPASE_I_1. 1 hit. PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_SALTY

Accession

Primary (citable) accession number: P0A1W2
Secondary accession number(s): P23697

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program