P0A1V9 (BLO2_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase OXA-2
EC=3.5.2.6

Alternative name(s):
Penicillinase

Gene names

Name:	bla
Synonyms:	oxa2

Encoded on

Plasmid IncN R46

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This is an oxacillin-hydrolyzing beta-lactamase.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-D beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro peptidoglycan-based cell wall biogenesis Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC penicillin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	By similarity
	Chain	22 – 275	254	Beta-lactamase OXA-2		PRO_0000017025
Regions
	Region	210 – 212	3	Substrate binding By similarity
Sites
	Active site	72	1	Acyl-ester intermediate By similarity
Amino acid modifications
	Modified residue	75	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A1V9 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: D3678DFCB78DE8B6	FASTA	275	31,686
	10 20 30 40 50 60 MAIRIFAILF SIFSLATFAH AQEGTLERSD WRKFFSEFQA KGTIVVADER QADRAMLVFD 70 80 90 100 110 120 PVRSKKRYSP ASTFKIPHTL FALDAGAVRD EFQIFRWDGV NRGFAGHNQD QDLRSAMRNS 130 140 150 160 170 180 TVWVYELFAK EIGDDKARRY LKKIDYGNAD PSTSNGDYWI EGSLAISAQE QIAFLRKLYR 190 200 210 220 230 240 NELPFRVEHQ RLVKDLMIVE AGRNWILRAK TGWEGRMGWW VGWVEWPTGS VFFALNIDTP 250 260 270 NRMDDLFKRE AIVRAILRSI EALPPNPAVN SDAAR

References

[1]	"The region of the IncN plasmid R46 coding for resistance to beta-lactam antibiotics, streptomycin/spectinomycin and sulphonamides is closely related to antibiotic resistance segments found in IncW plasmids and in Tn21-like transposons." Hall R.M., Vockler C. Nucleic Acids Res. 15:7491-7501(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The integron In1 in plasmid R46 includes two copies of the oxa2 gene cassette." Stokes H.W., Hall R.M. Plasmid 28:225-234(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M95287 Genomic DNA. Translation: AAB59082.1. M95287 Genomic DNA. Translation: AAB59084.2.
RefSeq	YP_006953608.1. NC_019081.1.
3D structure databases
ProteinModelPortal	P0A1V9.
SMR	P0A1V9. Positions 25-265.
ModBase	Search...
Proteomic databases
PRIDE	P0A1V9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	13906543.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR002137. Beta-lactam_class-D_AS. IPR001460. PCN-bd_Tpept. [Graphical view]
Pfam	PF00905. Transpeptidase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00337. BETA_LACTAMASE_D. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1744485.

Entry information

Entry name

BLO2_ECOLX

Accession

Primary (citable) accession number: P0A1V9
Secondary accession number(s): P05191, Q57015

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	April 3, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program