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P0A1V8

- BLO2_SALTM

UniProt

P0A1V8 - BLO2_SALTM

Protein

Beta-lactamase OXA-2

Gene

bla

Organism
Salmonella typhimurium
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
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    Functioni

    This is an oxacillin-hydrolyzing beta-lactamase.

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei72 – 721Acyl-ester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC
    2. penicillin binding Source: InterPro

    GO - Biological processi

    1. antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase OXA-2 (EC:3.5.2.6)
    Alternative name(s):
    Penicillinase
    Gene namesi
    Name:bla
    Synonyms:oxa2
    Encoded oniPlasmid IncN R463 Publications
    Plasmid pBP111 Publication
    OrganismiSalmonella typhimurium
    Taxonomic identifieri90371 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 21211 PublicationAdd
    BLAST
    Chaini22 – 275254Beta-lactamase OXA-2PRO_0000017026Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei75 – 751N6-carboxylysine1 Publication

    Proteomic databases

    PRIDEiP0A1V8.

    Structurei

    Secondary structure

    1
    275
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi29 – 313
    Helixi32 – 376
    Beta strandi42 – 487
    Beta strandi50 – 534
    Beta strandi57 – 604
    Helixi61 – 644
    Helixi71 – 744
    Helixi75 – 8410
    Turni105 – 1073
    Helixi113 – 1186
    Helixi122 – 13211
    Helixi134 – 14310
    Turni158 – 1603
    Beta strandi161 – 1633
    Helixi168 – 17912
    Beta strandi183 – 1853
    Helixi187 – 19610
    Beta strandi198 – 2025
    Beta strandi205 – 22622
    Beta strandi229 – 23810
    Helixi242 – 2476
    Helixi248 – 25912

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K38X-ray1.50A/B22-275[»]
    ProteinModelPortaliP0A1V8.
    SMRiP0A1V8. Positions 25-265.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A1V8.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni210 – 2123Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-D beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR002137. Beta-lactam_class-D_AS.
    IPR001460. PCN-bd_Tpept.
    [Graphical view]
    PfamiPF00905. Transpeptidase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00337. BETA_LACTAMASE_D. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0A1V8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAIRIFAILF SIFSLATFAH AQEGTLERSD WRKFFSEFQA KGTIVVADER    50
    QADRAMLVFD PVRSKKRYSP ASTFKIPHTL FALDAGAVRD EFQIFRWDGV 100
    NRGFAGHNQD QDLRSAMRNS TVWVYELFAK EIGDDKARRY LKKIDYGNAD 150
    PSTSNGDYWI EGSLAISAQE QIAFLRKLYR NELPFRVEHQ RLVKDLMIVE 200
    AGRNWILRAK TGWEGRMGWW VGWVEWPTGS VFFALNIDTP NRMDDLFKRE 250
    AIVRAILRSI EALPPNPAVN SDAAR 275
    Length:275
    Mass (Da):31,686
    Last modified:August 13, 1987 - v1
    Checksum:iD3678DFCB78DE8B6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M25261 Genomic DNA. Translation: AAA98357.1.
    X03037 Genomic DNA. Translation: CAA26839.1.
    X07260 Genomic DNA. Translation: CAA30246.1.
    PIRiA91350. PNEBT.
    RefSeqiNP_511223.1. NC_003292.1.
    NP_511225.1. NC_003292.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M25261 Genomic DNA. Translation: AAA98357.1 .
    X03037 Genomic DNA. Translation: CAA26839.1 .
    X07260 Genomic DNA. Translation: CAA30246.1 .
    PIRi A91350. PNEBT.
    RefSeqi NP_511223.1. NC_003292.1.
    NP_511225.1. NC_003292.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K38 X-ray 1.50 A/B 22-275 [» ]
    ProteinModelPortali P0A1V8.
    SMRi P0A1V8. Positions 25-265.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P0A1V8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P0A1V8.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR002137. Beta-lactam_class-D_AS.
    IPR001460. PCN-bd_Tpept.
    [Graphical view ]
    Pfami PF00905. Transpeptidase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00337. BETA_LACTAMASE_D. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the OXA2 beta-lactamase: comparison with other penicillin-reactive enzymes."
      Dale J.W., Godwin D., Mossakowska D., Stephenson P., Wall S.
      FEBS Lett. 191:39-44(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Type 1A.
      Plasmid: IncN R46
    2. "Oxacillin-hydrolysing beta-lactamases. A comparative analysis at nucleotide and amino acid sequence levels."
      Mossakowska D., Ali N.A., Dale J.W.
      Eur. J. Biochem. 180:309-318(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: IncN R46
    3. "Improved purification and characterization of the OXA-2 beta-lactamase."
      Holland S., Dale J.W.
      Biochem. J. 224:1009-1013(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 22-32.
      Plasmid: IncN R46
    4. "Nucleotide sequence of an OXA-2 beta-lactamase gene from the R-plasmid R1767 derived plasmid pBP11 and comparison to closely related resistance determinants found in R46 and Tn2603."
      Nuecken E.J., Henschke R.B., Schmidt F.R.J.
      J. Gen. Microbiol. 135:761-765(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: pBP11
    5. "Substrate-induced inactivation of the OXA2 beta-lactamase."
      Ledent P., Frere J.M.
      Biochem. J. 295:871-878(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 22-275, CARBAMYLATION AT LYS-75.

    Entry informationi

    Entry nameiBLO2_SALTM
    AccessioniPrimary (citable) accession number: P0A1V8
    Secondary accession number(s): P05191, Q57015
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3