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P0A1V8

- BLO2_SALTM

UniProt

P0A1V8 - BLO2_SALTM

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Protein

Beta-lactamase OXA-2

Gene

bla

Organism
Salmonella typhimurium
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This is an oxacillin-hydrolyzing beta-lactamase.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei72 – 721Acyl-ester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC
  2. penicillin binding Source: InterPro

GO - Biological processi

  1. antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase OXA-2 (EC:3.5.2.6)
Alternative name(s):
Penicillinase
Gene namesi
Name:bla
Synonyms:oxa2
Encoded oniPlasmid IncN R463 Publications
Plasmid pBP111 Publication
OrganismiSalmonella typhimurium
Taxonomic identifieri90371 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Chaini22 – 275254Beta-lactamase OXA-2PRO_0000017026Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei75 – 751N6-carboxylysine1 Publication

Proteomic databases

PRIDEiP0A1V8.

Interactioni

Structurei

Secondary structure

1
275
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 313Combined sources
Helixi32 – 376Combined sources
Beta strandi42 – 487Combined sources
Beta strandi50 – 534Combined sources
Beta strandi57 – 604Combined sources
Helixi61 – 644Combined sources
Helixi71 – 744Combined sources
Helixi75 – 8410Combined sources
Turni105 – 1073Combined sources
Helixi113 – 1186Combined sources
Helixi122 – 13211Combined sources
Helixi134 – 14310Combined sources
Turni158 – 1603Combined sources
Beta strandi161 – 1633Combined sources
Helixi168 – 17912Combined sources
Beta strandi183 – 1853Combined sources
Helixi187 – 19610Combined sources
Beta strandi198 – 2025Combined sources
Beta strandi205 – 22622Combined sources
Beta strandi229 – 23810Combined sources
Helixi242 – 2476Combined sources
Helixi248 – 25912Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K38X-ray1.50A/B22-275[»]
ProteinModelPortaliP0A1V8.
SMRiP0A1V8. Positions 25-265.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A1V8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni210 – 2123Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-D beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR002137. Beta-lactam_class-D_AS.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00337. BETA_LACTAMASE_D. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A1V8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAIRIFAILF SIFSLATFAH AQEGTLERSD WRKFFSEFQA KGTIVVADER
60 70 80 90 100
QADRAMLVFD PVRSKKRYSP ASTFKIPHTL FALDAGAVRD EFQIFRWDGV
110 120 130 140 150
NRGFAGHNQD QDLRSAMRNS TVWVYELFAK EIGDDKARRY LKKIDYGNAD
160 170 180 190 200
PSTSNGDYWI EGSLAISAQE QIAFLRKLYR NELPFRVEHQ RLVKDLMIVE
210 220 230 240 250
AGRNWILRAK TGWEGRMGWW VGWVEWPTGS VFFALNIDTP NRMDDLFKRE
260 270
AIVRAILRSI EALPPNPAVN SDAAR
Length:275
Mass (Da):31,686
Last modified:August 13, 1987 - v1
Checksum:iD3678DFCB78DE8B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25261 Genomic DNA. Translation: AAA98357.1.
X03037 Genomic DNA. Translation: CAA26839.1.
X07260 Genomic DNA. Translation: CAA30246.1.
PIRiA91350. PNEBT.
RefSeqiNP_511223.1. NC_003292.1.
NP_511225.1. NC_003292.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25261 Genomic DNA. Translation: AAA98357.1 .
X03037 Genomic DNA. Translation: CAA26839.1 .
X07260 Genomic DNA. Translation: CAA30246.1 .
PIRi A91350. PNEBT.
RefSeqi NP_511223.1. NC_003292.1.
NP_511225.1. NC_003292.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1K38 X-ray 1.50 A/B 22-275 [» ]
ProteinModelPortali P0A1V8.
SMRi P0A1V8. Positions 25-265.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P0A1V8.

Proteomic databases

PRIDEi P0A1V8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P0A1V8.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR002137. Beta-lactam_class-D_AS.
IPR001460. PCN-bd_Tpept.
[Graphical view ]
Pfami PF00905. Transpeptidase. 1 hit.
[Graphical view ]
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00337. BETA_LACTAMASE_D. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of the OXA2 beta-lactamase: comparison with other penicillin-reactive enzymes."
    Dale J.W., Godwin D., Mossakowska D., Stephenson P., Wall S.
    FEBS Lett. 191:39-44(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Type 1A.
    Plasmid: IncN R46
  2. "Oxacillin-hydrolysing beta-lactamases. A comparative analysis at nucleotide and amino acid sequence levels."
    Mossakowska D., Ali N.A., Dale J.W.
    Eur. J. Biochem. 180:309-318(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: IncN R46
  3. "Improved purification and characterization of the OXA-2 beta-lactamase."
    Holland S., Dale J.W.
    Biochem. J. 224:1009-1013(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 22-32.
    Plasmid: IncN R46
  4. "Nucleotide sequence of an OXA-2 beta-lactamase gene from the R-plasmid R1767 derived plasmid pBP11 and comparison to closely related resistance determinants found in R46 and Tn2603."
    Nuecken E.J., Henschke R.B., Schmidt F.R.J.
    J. Gen. Microbiol. 135:761-765(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pBP11
  5. "Substrate-induced inactivation of the OXA2 beta-lactamase."
    Ledent P., Frere J.M.
    Biochem. J. 295:871-878(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 22-275, CARBAMYLATION AT LYS-75.

Entry informationi

Entry nameiBLO2_SALTM
AccessioniPrimary (citable) accession number: P0A1V8
Secondary accession number(s): P05191, Q57015
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: November 26, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3