Reviewed, UniProtKB/Swiss-Prot P0A1P1 (G3P1_SALTI)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase
      Short name=GAPDH
    EC=1.2.1.12

Gene names

Name:	gapA
Synonyms:	gap
Ordered Locus Names:	STY1825, t1169

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 331	330	Glyceraldehyde-3-phosphate dehydrogenase		PRO_0000145676
Regions
	Nucleotide binding	12 – 13	2	NAD By similarity
	Region	149 – 151	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	209 – 210	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	150	1	Nucleophile By similarity
	Binding site	34	1	NAD By similarity
	Binding site	78	1	NAD; via carbonyl oxygen By similarity
	Binding site	180	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	232	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	314	1	NAD By similarity
	Site	177	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A1P1-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 872BA8D616C807B0	FASTA	331	35,587
	10 20 30 40 50 60 MTIKVGINGF GRIGRIVFRA AQKRSDIEIV AINDLLDAEY MAYMLKYDST HGRFDGTVEV 70 80 90 100 110 120 KDGHLIVNGK KIRVTAERDP ANLKWDEVGV DVVAEATGIF LTDETARKHI TAGAKKVVLT 130 140 150 160 170 180 GPSKDNTPMF VKGANFDKYE GQDIVSNASC TTNCLAPLAK VINDNFGIIE GLMTTVHATT 190 200 210 220 230 240 ATQKTVDGPS HKDWRGGRGA SQNIIPSSTG AAKAVGKVLP ELNGKLTGMA FRVPTPNVSV 250 260 270 280 290 300 VDLTVRLEKA ATYEQIKAAV KAAAEGEMKG VLGYTEDDVV STDFNGEVCT SVFDAKAGIA 310 320 330 LNDNFVKLVS WYDNETGYSN KVLDLIAHIS K

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL627271 Genomic DNA. Translation: CAD02064.1. AE014613 Genomic DNA. Translation: AAO68826.1.
RefSeq	NP_456222.1. NP_804977.1.
3D structure databases
SMR	P0A1P1. Positions 2-331.
ModBase	Search...
Proteomic databases
PRIDE	P0A1P1.
Genome annotation databases
GeneID	1070587. 1248193.
GenomeReviews	Gene locus t1169 in contig AE014613_GR.
KEGG	stt:t1169. sty:STY1825.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571736.
OMA	NIKWADA.
Enzyme and pathway databases
BioCyc	SENT209261:T1169-MONOMER. SENT220341:STY1825-MONOMER.
BRENDA	1.2.1.12. 3716.
Family and domain databases
InterPro	IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020832. GlycerAld_3-P_DH_cat_sub. IPR020831. GlycerAld_3-P_DH_family. IPR020828. GlycerAld_3-P_DH_NAD(P)_bd. IPR000173. GlycerAld_3-P_DH_subfam. IPR006424. Glyceraldehyde-3-P_DH_1. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P1_SALTI

Accession

Primary (citable) accession number: P0A1P1
Secondary accession number(s): P24165

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)