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Protein

Flagellar protein FliT

Gene

fliT

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Dual-function protein that regulates the transcription of class 2 flagellar operons and that also acts as an export chaperone for the filament-capping protein FliD. As a transcriptional regulator, acts as an anti-FlhDC factor; it directly binds FlhC, thus inhibiting the binding of the FlhC/FlhD complex to class 2 promoters, resulting in decreased expression of class 2 flagellar operons. As a chaperone, effects FliD transition to the membrane by preventing its premature polymerization, and by directing it to the export apparatus.4 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Repressor

Keywords - Biological processi

Bacterial flagellum biogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciSENT99287:GCTI-1973-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flagellar protein FliT
Gene namesi
Name:fliT
Ordered Locus Names:STM1962
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 122122Flagellar protein FliTPRO_0000180977Add
BLAST

Proteomic databases

PaxDbiP0A1N2.
PRIDEiP0A1N2.

Interactioni

Subunit structurei

Homodimer. Interacts with FliD and FlhC.2 Publications

Protein-protein interaction databases

DIPiDIP-60504N.
STRINGi99287.STM1962.

Structurei

Secondary structure

1
122
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 2723Combined sources
Helixi30 – 4819Combined sources
Helixi58 – 9336Combined sources
Helixi97 – 11014Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A7MX-ray3.20A/B1-122[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A1N2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 5050Required for homodimerizationAdd
BLAST
Regioni60 – 9839FliD bindingAdd
BLAST

Sequence similaritiesi

Belongs to the FliT family.Curated

Phylogenomic databases

eggNOGiENOG4105YXP. Bacteria.
ENOG41124U3. LUCA.
HOGENOMiHOG000266968.
KOiK02423.
OMAiDMEITYL.
OrthoDBiEOG66QM0W.

Family and domain databases

HAMAPiMF_01180. FliT.
InterProiIPR008622. FliT.
[Graphical view]
PfamiPF05400. FliT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A1N2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSTVEFINR WQRIALLSQS LLELAQRGEW DLLLQQEVSY LQSIETVMEK
60 70 80 90 100
QTPPGITRSI QDMVAGYIKQ TLDNEQLLKG LLQQRLDELS SLIGQSTRQK
110 120
SLNNAYGRLS GMLLVPDAPG AS
Length:122
Mass (Da):13,705
Last modified:March 15, 2005 - v1
Checksum:iCF622E06DCD122AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85241 Genomic DNA. Translation: AAA27078.1.
L01643 Genomic DNA. Translation: AAA27109.1.
AE006468 Genomic DNA. Translation: AAL20874.1.
RefSeqiNP_460915.1. NC_003197.1.
WP_000204899.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20874; AAL20874; STM1962.
GeneIDi1253483.
KEGGistm:STM1962.
PATRICi32382485. VBISalEnt20916_2078.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85241 Genomic DNA. Translation: AAA27078.1.
L01643 Genomic DNA. Translation: AAA27109.1.
AE006468 Genomic DNA. Translation: AAL20874.1.
RefSeqiNP_460915.1. NC_003197.1.
WP_000204899.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A7MX-ray3.20A/B1-122[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60504N.
STRINGi99287.STM1962.

Proteomic databases

PaxDbiP0A1N2.
PRIDEiP0A1N2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20874; AAL20874; STM1962.
GeneIDi1253483.
KEGGistm:STM1962.
PATRICi32382485. VBISalEnt20916_2078.

Phylogenomic databases

eggNOGiENOG4105YXP. Bacteria.
ENOG41124U3. LUCA.
HOGENOMiHOG000266968.
KOiK02423.
OMAiDMEITYL.
OrthoDBiEOG66QM0W.

Enzyme and pathway databases

BioCyciSENT99287:GCTI-1973-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A1N2.

Family and domain databases

HAMAPiMF_01180. FliT.
InterProiIPR008622. FliT.
[Graphical view]
PfamiPF05400. FliT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Subdivision of flagellar region III of the Escherichia coli and Salmonella typhimurium chromosomes and identification of two additional flagellar genes."
    Kawagishi I., Mueller V., Williams A.W., Irikura V.M., Macnab R.M.
    J. Gen. Microbiol. 138:1051-1065(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SJW1103.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  3. "Two novel regulatory genes, fliT and fliZ, in the flagellar regulon of Salmonella."
    Kutsukake K., Ikebe T., Yamamoto S.
    Genes Genet. Syst. 74:287-292(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN THE REGULATION OF THE FLAGELLAR REGULON.
    Strain: LT2 / SGSC1412 / ATCC 700720.
  4. "Substrate-specific binding of hook-associated proteins by FlgN and FliT, putative chaperones for flagellum assembly."
    Fraser G.M., Bennett J.C., Hughes C.
    Mol. Microbiol. 32:569-580(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A CHAPERONE, INTERACTION WITH FLID.
    Strain: SJW1103.
  5. "Substrate complexes and domain organization of the Salmonella flagellar export chaperones FlgN and FliT."
    Bennett J.C., Thomas J., Fraser G.M., Hughes C.
    Mol. Microbiol. 39:781-791(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A CHAPERONE, SUBUNIT, INTERACTION WITH FLID.
    Strain: SJW1103.
  6. "FliT acts as an anti-FlhD2C2 factor in the transcriptional control of the flagellar regulon in Salmonella enterica serovar typhimurium."
    Yamamoto S., Kutsukake K.
    J. Bacteriol. 188:6703-6708(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A TRANSCRIPTIONAL REGULATOR OF THE FLAGELLAR REGULON.
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiFLIT_SALTY
AccessioniPrimary (citable) accession number: P0A1N2
Secondary accession number(s): P26611
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 11, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.