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Protein

Surface presentation of antigens protein SpaS

Gene

spaS

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the ipa antigens. Required for invasion and for secretion of the three ipa proteins.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Virulence

Protein family/group databases

MEROPSiN06.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Surface presentation of antigens protein SpaS
Alternative name(s):
Spa40 protein
Gene namesi
Name:spaS
Synonyms:spa40
Ordered Locus Names:CP0156
Encoded oniPlasmid pWR1002 Publications
Plasmid pMYSH60001 Publication
Plasmid pCP3011 Publication
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
Proteomesi
  • UP000001006 Componenti: Plasmid pCP301

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei28 – 4821HelicalSequence analysisAdd
BLAST
Transmembranei70 – 9021HelicalSequence analysisAdd
BLAST
Transmembranei133 – 15321HelicalSequence analysisAdd
BLAST
Transmembranei158 – 17821HelicalSequence analysisAdd
BLAST
Transmembranei181 – 20121HelicalSequence analysisAdd
BLAST
Transmembranei260 – 28021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342Surface presentation of antigens protein SpaSPRO_0000180957Add
BLAST

Proteomic databases

PaxDbiP0A1M8.
PRIDEiP0A1M8.

Interactioni

Protein-protein interaction databases

STRINGi198214.CP0156.

Structurei

Secondary structure

1
342
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi242 – 2498Combined sources
Beta strandi252 – 2565Combined sources
Beta strandi260 – 2667Combined sources
Turni269 – 2713Combined sources
Beta strandi276 – 2827Combined sources
Helixi283 – 29513Combined sources
Beta strandi300 – 3023Combined sources
Helixi304 – 31310Combined sources
Beta strandi316 – 3194Combined sources
Turni322 – 3243Combined sources
Helixi325 – 33713Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VT1X-ray2.00A207-257[»]
B258-342[»]
ProteinModelPortaliP0A1M8.
SMRiP0A1M8. Positions 258-338.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A1M8.

Family & Domainsi

Sequence similaritiesi

Belongs to the type III secretion exporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108M6W. Bacteria.
COG1377. LUCA.
HOGENOMiHOG000231600.
KOiK03229.
OMAiQPNPGAY.

Family and domain databases

Gene3Di3.40.1690.10. 1 hit.
InterProiIPR006307. BsaZ.
IPR006135. T3SS_substrate_exporter.
IPR029025. T3SS_substrate_exporter_C.
[Graphical view]
PANTHERiPTHR30531. PTHR30531. 1 hit.
PfamiPF01312. Bac_export_2. 1 hit.
[Graphical view]
PRINTSiPR00950. TYPE3IMSPROT.
SUPFAMiSSF160544. SSF160544. 1 hit.
TIGRFAMsiTIGR01404. FlhB_rel_III. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A1M8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANKTEKPTP KKLKDAAKKG QSFKFKDLTT VVIILVGTFT IISFFSLSDV
60 70 80 90 100
MLLYRYVIIN DFEINEGKYF FAVVIVFFKI IGFPLFFCVL SAVLPTLVQT
110 120 130 140 150
KFVLATKAIK IDFSVLNPVK GLKKIFSIKT IKEFFKSILL LIILALTTYF
160 170 180 190 200
FWINDRKIIF SQVFSSVDGL YLIWGRLFKD IILFFLAFSI LVIILDFVIE
210 220 230 240 250
FILYMKDMMM DKQEIKREYI EQEGHFETKS RRRELHIEIL SEQTKSDIRN
260 270 280 290 300
SKLVVMNPTH IAIGIYFNPE IAPAPFISLI ETNQCALAVR KYANEVGIPT
310 320 330 340
VRDVKLARKL YKTHTKYSFV DFEHLDEVLR LIVWLEQVEN TH
Length:342
Mass (Da):39,852
Last modified:March 1, 2005 - v1
Checksum:i48BBF531BFE034BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13663 Genomic DNA. Translation: BAA02832.1.
AL391753 Genomic DNA. Translation: CAC05831.1.
AF348706 Genomic DNA. Translation: AAK18475.1.
AF386526 Genomic DNA. Translation: AAL72303.1.
PIRiA38908.
RefSeqiNP_085319.1. NC_002698.1.
NP_858289.1. NC_004851.1.
YP_009062513.1. NC_024996.1.

Genome annotation databases

EnsemblBacteriaiAAL72303; AAL72303; SF_p0156.
GeneIDi876433.
KEGGipg:20467610.
pg:876433.
sfl:CP0156.
PATRICi18722621. VBIShiFle86970_5401.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13663 Genomic DNA. Translation: BAA02832.1.
AL391753 Genomic DNA. Translation: CAC05831.1.
AF348706 Genomic DNA. Translation: AAK18475.1.
AF386526 Genomic DNA. Translation: AAL72303.1.
PIRiA38908.
RefSeqiNP_085319.1. NC_002698.1.
NP_858289.1. NC_004851.1.
YP_009062513.1. NC_024996.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VT1X-ray2.00A207-257[»]
B258-342[»]
ProteinModelPortaliP0A1M8.
SMRiP0A1M8. Positions 258-338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198214.CP0156.

Protein family/group databases

MEROPSiN06.002.

Proteomic databases

PaxDbiP0A1M8.
PRIDEiP0A1M8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL72303; AAL72303; SF_p0156.
GeneIDi876433.
KEGGipg:20467610.
pg:876433.
sfl:CP0156.
PATRICi18722621. VBIShiFle86970_5401.

Phylogenomic databases

eggNOGiENOG4108M6W. Bacteria.
COG1377. LUCA.
HOGENOMiHOG000231600.
KOiK03229.
OMAiQPNPGAY.

Miscellaneous databases

EvolutionaryTraceiP0A1M8.

Family and domain databases

Gene3Di3.40.1690.10. 1 hit.
InterProiIPR006307. BsaZ.
IPR006135. T3SS_substrate_exporter.
IPR029025. T3SS_substrate_exporter_C.
[Graphical view]
PANTHERiPTHR30531. PTHR30531. 1 hit.
PfamiPF01312. Bac_export_2. 1 hit.
[Graphical view]
PRINTSiPR00950. TYPE3IMSPROT.
SUPFAMiSSF160544. SSF160544. 1 hit.
TIGRFAMsiTIGR01404. FlhB_rel_III. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSPAS_SHIFL
AccessioniPrimary (citable) accession number: P0A1M8
Secondary accession number(s): P40707
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: September 7, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.