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Protein

Surface presentation of antigens protein SpaS

Gene

spaS

Organism
Shigella flexneri
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the ipa antigens. Required for invasion and for secretion of the three ipa proteins.

GO - Biological processi

Keywordsi

Biological processVirulence

Protein family/group databases

MEROPSiN06.002
TCDBi3.A.6.1.2 the type iii (virulence-related) secretory pathway (iiisp) family

Names & Taxonomyi

Protein namesi
Recommended name:
Surface presentation of antigens protein SpaS
Alternative name(s):
Spa40 protein
Gene namesi
Name:spaS
Synonyms:spa40
Ordered Locus Names:CP0156
Encoded oniPlasmid pWR1002 Publications
Plasmid pMYSH60001 Publication
Plasmid pCP3011 Publication
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000001006 Componenti: Plasmid pCP301

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei28 – 48HelicalSequence analysisAdd BLAST21
Transmembranei70 – 90HelicalSequence analysisAdd BLAST21
Transmembranei133 – 153HelicalSequence analysisAdd BLAST21
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Transmembranei181 – 201HelicalSequence analysisAdd BLAST21
Transmembranei260 – 280HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001809571 – 342Surface presentation of antigens protein SpaSAdd BLAST342

Proteomic databases

PRIDEiP0A1M8

Structurei

Secondary structure

1342
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi242 – 249Combined sources8
Beta strandi252 – 256Combined sources5
Beta strandi260 – 266Combined sources7
Turni269 – 271Combined sources3
Beta strandi276 – 282Combined sources7
Helixi283 – 295Combined sources13
Beta strandi300 – 302Combined sources3
Helixi304 – 313Combined sources10
Beta strandi316 – 319Combined sources4
Turni322 – 324Combined sources3
Helixi325 – 337Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VT1X-ray2.00A207-257[»]
B258-342[»]
ProteinModelPortaliP0A1M8
SMRiP0A1M8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A1M8

Family & Domainsi

Sequence similaritiesi

Belongs to the type III secretion exporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108M6W Bacteria
COG1377 LUCA
HOGENOMiHOG000231600
KOiK22510
OMAiQPNPGAY

Family and domain databases

Gene3Di3.40.1690.10, 1 hit
InterProiView protein in InterPro
IPR006307 BsaZ
IPR006135 T3SS_substrate_exporter
IPR029025 T3SS_substrate_exporter_C
PANTHERiPTHR30531 PTHR30531, 1 hit
PfamiView protein in Pfam
PF01312 Bac_export_2, 1 hit
PRINTSiPR00950 TYPE3IMSPROT
SUPFAMiSSF160544 SSF160544, 1 hit
TIGRFAMsiTIGR01404 FlhB_rel_III, 1 hit

Sequencei

Sequence statusi: Complete.

P0A1M8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANKTEKPTP KKLKDAAKKG QSFKFKDLTT VVIILVGTFT IISFFSLSDV
60 70 80 90 100
MLLYRYVIIN DFEINEGKYF FAVVIVFFKI IGFPLFFCVL SAVLPTLVQT
110 120 130 140 150
KFVLATKAIK IDFSVLNPVK GLKKIFSIKT IKEFFKSILL LIILALTTYF
160 170 180 190 200
FWINDRKIIF SQVFSSVDGL YLIWGRLFKD IILFFLAFSI LVIILDFVIE
210 220 230 240 250
FILYMKDMMM DKQEIKREYI EQEGHFETKS RRRELHIEIL SEQTKSDIRN
260 270 280 290 300
SKLVVMNPTH IAIGIYFNPE IAPAPFISLI ETNQCALAVR KYANEVGIPT
310 320 330 340
VRDVKLARKL YKTHTKYSFV DFEHLDEVLR LIVWLEQVEN TH
Length:342
Mass (Da):39,852
Last modified:March 1, 2005 - v1
Checksum:i48BBF531BFE034BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13663 Genomic DNA Translation: BAA02832.1
AL391753 Genomic DNA Translation: CAC05831.1
AF348706 Genomic DNA Translation: AAK18475.1
AF386526 Genomic DNA Translation: AAL72303.1
PIRiA38908
RefSeqiNP_085319.1, NC_002698.1
NP_858289.1, NC_004851.1
WP_001284686.1, NZ_MTPK01000040.1
YP_009062513.1, NC_024996.1

Genome annotation databases

EnsemblBacteriaiAAL72303; AAL72303; SF_p0156
GeneIDi1237999
876433
KEGGisfl:CP0156
PATRICifig|198214.7.peg.5401

Similar proteinsi

Entry informationi

Entry nameiSPAS_SHIFL
AccessioniPrimary (citable) accession number: P0A1M8
Secondary accession number(s): P40707
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid, Reference proteome

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