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P0A1I5

- MXIA_SHIFL

UniProt

P0A1I5 - MXIA_SHIFL

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Protein

Protein MxiA

Gene

mxiA

Organism
Shigella flexneri
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Necessary for the secretion of IPA invasins.

GO - Biological processi

  1. pathogenesis Source: UniProtKB-KW
  2. protein secretion Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport, Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Protein MxiA
Alternative name(s):
Protein VirH
Gene namesi
Name:mxiA
Synonyms:virH
Ordered Locus Names:CP0147
Encoded oniPlasmid pWR1004 Publications
Plasmid pMYSH60002 Publications
Plasmid pCP3011 Publication
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000001006: Plasmid pCP301

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei28 – 5225HelicalSequence AnalysisAdd
BLAST
Transmembranei105 – 12925HelicalSequence AnalysisAdd
BLAST
Transmembranei197 – 21620HelicalSequence AnalysisAdd
BLAST
Transmembranei232 – 25625HelicalSequence AnalysisAdd
BLAST
Transmembranei274 – 29219HelicalSequence AnalysisAdd
BLAST
Transmembranei299 – 31517HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 686686Protein MxiAPRO_0000190032Add
BLAST

Proteomic databases

PRIDEiP0A1I5.

Interactioni

Protein-protein interaction databases

STRINGi198214.CP0147.

Structurei

Secondary structure

1
686
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi362 – 3687Combined sources
Helixi377 – 39115Combined sources
Beta strandi400 – 4078Combined sources
Beta strandi409 – 4168Combined sources
Beta strandi419 – 4257Combined sources
Beta strandi430 – 4334Combined sources
Beta strandi448 – 4525Combined sources
Beta strandi455 – 4617Combined sources
Helixi478 – 49114Combined sources
Helixi494 – 4963Combined sources
Helixi500 – 51112Combined sources
Helixi515 – 5228Combined sources
Helixi527 – 53812Combined sources
Turni539 – 5413Combined sources
Helixi547 – 55711Combined sources
Turni558 – 5603Combined sources
Helixi564 – 57411Combined sources
Helixi576 – 5838Combined sources
Beta strandi588 – 5947Combined sources
Helixi596 – 6049Combined sources
Helixi623 – 63715Combined sources
Beta strandi638 – 6403Combined sources
Beta strandi644 – 6474Combined sources
Turni649 – 6513Combined sources
Helixi652 – 6609Combined sources
Beta strandi664 – 6663Combined sources
Beta strandi668 – 6703Combined sources
Beta strandi679 – 6868Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4A5PX-ray3.15A/B/C318-686[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4789.
HOGENOMiHOG000254015.
KOiK03230.
OMAiYNERVIS.
OrthoDBiEOG6RZB0J.

Family and domain databases

InterProiIPR025505. FHIPEP_CS.
IPR001712. T3SS_FHIPEP.
IPR006302. T3SS_HrcV.
[Graphical view]
PfamiPF00771. FHIPEP. 1 hit.
[Graphical view]
PIRSFiPIRSF005419. FlhA. 1 hit.
PRINTSiPR00949. TYPE3IMAPROT.
TIGRFAMsiTIGR01399. hrcV. 1 hit.
PROSITEiPS00994. FHIPEP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A1I5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIQSFLKQVS TKPELIILVL MVMIIAMLII PLPTYLVDFL IGLNIVLAIL
60 70 80 90 100
VFMGSFYIER ILSFSTFPSV LLITTLFRLA LSISTSRLIL VDADAGKIIT
110 120 130 140 150
TFGQFVIGDS LAVGFVIFSI VTVVQFIVIT KGSERVAEVA ARFSLDGMPG
160 170 180 190 200
KQMSIDADLK AGIIDAAGAK ERRSILERES QLYGSFDGAM KFIKGDAIAG
210 220 230 240 250
IIIIFVNLIG GISVGMSQHG MSLSGALSTY TILTIGDGLV SQIPALLISI
260 270 280 290 300
SAGFIVTRVN GDSDNMGRNI MSQIFGNPFV LIVTSALALA IGMLPGFPFF
310 320 330 340 350
VFFLIAVTLT ALFYYKKVVE KEKSLSESDS SGYTGTFDID NSHDSSLAMI
360 370 380 390 400
ENLDAISSET VPLILLFAEN KINANDMEGL IERIRSQFFI DYGVRLPTIL
410 420 430 440 450
YRTSNELKVD DIVLLINEVR ADSFNIYFDK VCITDENGDI DALGIPVVST
460 470 480 490 500
SYNERVISWV DVSYTENLTN IDAKIKSAQD EFYHQLSQAL LNNINEIFGI
510 520 530 540 550
QETKNMLDQF ENRYPDLLKE VFRHVTIQRI SEVLQRLLGE NISVRNLKLI
560 570 580 590 600
MESLALWAPR EKDVITLVEH VRASLSRYIC SKIAVSGEIK VVMLSGYIED
610 620 630 640 650
AIRKGIRQTS GGSFLNMDIE VSDEVMETLA HALRELRNAK KNFVLLVSVD
660 670 680
IRRFVKRLID NRFKSILVIS YAEIDEAYTI NVLKTI
Length:686
Mass (Da):76,145
Last modified:March 1, 2005 - v1
Checksum:iBD675C2C3BED820F
GO

Sequence cautioni

The sequence AAA26529.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti95 – 951A → R in AAA26529. (PubMed:1639496)Curated
Sequence conflicti255 – 2562IV → ML in AAA26529. (PubMed:1639496)Curated
Sequence conflicti342 – 3421S → T in AAA26529. (PubMed:1639496)Curated
Sequence conflicti355 – 3551A → R in AAA26529. (PubMed:1639496)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91664 Genomic DNA. Translation: AAA26529.1. Different initiation.
D10999 Genomic DNA. Translation: BAA01771.1.
AL391753 Genomic DNA. Translation: CAC05822.1.
AF348706 Genomic DNA. Translation: AAK18466.1.
AF386526 Genomic DNA. Translation: AAL72333.1.
M81458 Genomic DNA. No translation available.
PIRiA44797.
RefSeqiNP_085310.1. NC_002698.1.
NP_858280.1. NC_004851.1.

Genome annotation databases

EnsemblBacteriaiAAL72333; AAL72333; CP0147.
GeneIDi1238036.
876517.
KEGGisfl:CP0147.
PATRICi18722607. VBIShiFle86970_5394.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91664 Genomic DNA. Translation: AAA26529.1 . Different initiation.
D10999 Genomic DNA. Translation: BAA01771.1 .
AL391753 Genomic DNA. Translation: CAC05822.1 .
AF348706 Genomic DNA. Translation: AAK18466.1 .
AF386526 Genomic DNA. Translation: AAL72333.1 .
M81458 Genomic DNA. No translation available.
PIRi A44797.
RefSeqi NP_085310.1. NC_002698.1.
NP_858280.1. NC_004851.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4A5P X-ray 3.15 A/B/C 318-686 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 198214.CP0147.

Proteomic databases

PRIDEi P0A1I5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL72333 ; AAL72333 ; CP0147 .
GeneIDi 1238036.
876517.
KEGGi sfl:CP0147.
PATRICi 18722607. VBIShiFle86970_5394.

Phylogenomic databases

eggNOGi COG4789.
HOGENOMi HOG000254015.
KOi K03230.
OMAi YNERVIS.
OrthoDBi EOG6RZB0J.

Family and domain databases

InterProi IPR025505. FHIPEP_CS.
IPR001712. T3SS_FHIPEP.
IPR006302. T3SS_HrcV.
[Graphical view ]
Pfami PF00771. FHIPEP. 1 hit.
[Graphical view ]
PIRSFi PIRSF005419. FlhA. 1 hit.
PRINTSi PR00949. TYPE3IMAPROT.
TIGRFAMsi TIGR01399. hrcV. 1 hit.
PROSITEi PS00994. FHIPEP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "mxiA of Shigella flexneri 2a, which facilitates export of invasion plasmid antigens, encodes a homolog of the low-calcium-response protein, LcrD, of Yersinia pestis."
    Andrews G.P., Maurelli A.T.
    Infect. Immun. 60:3287-3295(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.
    Plasmid: pWR100
  2. "A virulence locus, virH, on the large plasmid of Shigella flexneri."
    Sasakawa C.
    Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YSH6000 / Serotype 2a.
    Plasmid: pMYSH6000
  3. "The virulence plasmid pWR100 and the repertoire of proteins secreted by the type III secretion apparatus of Shigella flexneri."
    Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H., Kunst F., Sansonetti P.J., Parsot C.
    Mol. Microbiol. 38:760-771(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: M90T / Serotype 5a.
    Plasmid: pWR100
  4. "Complete DNA sequence and analysis of the large virulence plasmid of Shigella flexneri."
    Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V., Blattner F.R.
    Infect. Immun. 69:3271-3285(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: M90T / Serotype 5a.
    Plasmid: pWR100
  5. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
    Plasmid: pCP301
  6. "Surface presentation of Shigella flexneri invasion plasmid antigens requires the products of the spa locus."
    Venkatesan M.M., Buysse J.M., Oaks E.V.
    J. Bacteriol. 174:1990-2001(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 489-686.
    Strain: M90T / Serotype 5a.
    Plasmid: pWR100
  7. "Eight genes in region 5 that form an operon are essential for invasion of epithelial cells by Shigella flexneri 2a."
    Sasakawa C., Komatsu K., Tobe T., Suzuki T., Yoshikawa M.
    J. Bacteriol. 175:2334-2346(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 489-686.
    Strain: YSH6000 / Serotype 2a.
    Plasmid: pMYSH6000

Entry informationi

Entry nameiMXIA_SHIFL
AccessioniPrimary (citable) accession number: P0A1I5
Secondary accession number(s): P35533, Q55295
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: November 26, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3