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P0A1I5

- MXIA_SHIFL

UniProt

P0A1I5 - MXIA_SHIFL

Protein

Protein MxiA

Gene

mxiA

Organism
Shigella flexneri
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (01 Mar 2005)
      Previous versions | rss
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    Functioni

    Necessary for the secretion of IPA invasins.

    GO - Biological processi

    1. pathogenesis Source: UniProtKB-KW
    2. protein secretion Source: InterPro

    Keywords - Biological processi

    Protein transport, Transport, Virulence

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein MxiA
    Alternative name(s):
    Protein VirH
    Gene namesi
    Name:mxiA
    Synonyms:virH
    Ordered Locus Names:CP0147
    Encoded oniPlasmid pWR1004 Publications
    Plasmid pMYSH60002 Publications
    Plasmid pCP3011 Publication
    OrganismiShigella flexneri
    Taxonomic identifieri623 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
    ProteomesiUP000001006: Plasmid pCP301

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 686686Protein MxiAPRO_0000190032Add
    BLAST

    Proteomic databases

    PRIDEiP0A1I5.

    Interactioni

    Protein-protein interaction databases

    STRINGi198214.CP0147.

    Structurei

    Secondary structure

    1
    686
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi362 – 3687
    Helixi377 – 39115
    Beta strandi400 – 4078
    Beta strandi409 – 4168
    Beta strandi419 – 4257
    Beta strandi430 – 4334
    Beta strandi448 – 4525
    Beta strandi455 – 4617
    Helixi478 – 49114
    Helixi494 – 4963
    Helixi500 – 51112
    Helixi515 – 5228
    Helixi527 – 53812
    Turni539 – 5413
    Helixi547 – 55711
    Turni558 – 5603
    Helixi564 – 57411
    Helixi576 – 5838
    Beta strandi588 – 5947
    Helixi596 – 6049
    Helixi623 – 63715
    Beta strandi638 – 6403
    Beta strandi644 – 6474
    Turni649 – 6513
    Helixi652 – 6609
    Beta strandi664 – 6663
    Beta strandi668 – 6703
    Beta strandi679 – 6868

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4A5PX-ray3.15A/B/C318-686[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei28 – 5225HelicalSequence AnalysisAdd
    BLAST
    Transmembranei105 – 12925HelicalSequence AnalysisAdd
    BLAST
    Transmembranei197 – 21620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei232 – 25625HelicalSequence AnalysisAdd
    BLAST
    Transmembranei274 – 29219HelicalSequence AnalysisAdd
    BLAST
    Transmembranei299 – 31517HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG4789.
    HOGENOMiHOG000254015.
    KOiK03230.
    OMAiYNERVIS.
    OrthoDBiEOG6RZB0J.

    Family and domain databases

    InterProiIPR025505. FHIPEP_CS.
    IPR001712. T3SS_FHIPEP.
    IPR006302. T3SS_HrcV.
    [Graphical view]
    PfamiPF00771. FHIPEP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005419. FlhA. 1 hit.
    PRINTSiPR00949. TYPE3IMAPROT.
    TIGRFAMsiTIGR01399. hrcV. 1 hit.
    PROSITEiPS00994. FHIPEP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A1I5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIQSFLKQVS TKPELIILVL MVMIIAMLII PLPTYLVDFL IGLNIVLAIL    50
    VFMGSFYIER ILSFSTFPSV LLITTLFRLA LSISTSRLIL VDADAGKIIT 100
    TFGQFVIGDS LAVGFVIFSI VTVVQFIVIT KGSERVAEVA ARFSLDGMPG 150
    KQMSIDADLK AGIIDAAGAK ERRSILERES QLYGSFDGAM KFIKGDAIAG 200
    IIIIFVNLIG GISVGMSQHG MSLSGALSTY TILTIGDGLV SQIPALLISI 250
    SAGFIVTRVN GDSDNMGRNI MSQIFGNPFV LIVTSALALA IGMLPGFPFF 300
    VFFLIAVTLT ALFYYKKVVE KEKSLSESDS SGYTGTFDID NSHDSSLAMI 350
    ENLDAISSET VPLILLFAEN KINANDMEGL IERIRSQFFI DYGVRLPTIL 400
    YRTSNELKVD DIVLLINEVR ADSFNIYFDK VCITDENGDI DALGIPVVST 450
    SYNERVISWV DVSYTENLTN IDAKIKSAQD EFYHQLSQAL LNNINEIFGI 500
    QETKNMLDQF ENRYPDLLKE VFRHVTIQRI SEVLQRLLGE NISVRNLKLI 550
    MESLALWAPR EKDVITLVEH VRASLSRYIC SKIAVSGEIK VVMLSGYIED 600
    AIRKGIRQTS GGSFLNMDIE VSDEVMETLA HALRELRNAK KNFVLLVSVD 650
    IRRFVKRLID NRFKSILVIS YAEIDEAYTI NVLKTI 686
    Length:686
    Mass (Da):76,145
    Last modified:March 1, 2005 - v1
    Checksum:iBD675C2C3BED820F
    GO

    Sequence cautioni

    The sequence AAA26529.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti95 – 951A → R in AAA26529. (PubMed:1639496)Curated
    Sequence conflicti255 – 2562IV → ML in AAA26529. (PubMed:1639496)Curated
    Sequence conflicti342 – 3421S → T in AAA26529. (PubMed:1639496)Curated
    Sequence conflicti355 – 3551A → R in AAA26529. (PubMed:1639496)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91664 Genomic DNA. Translation: AAA26529.1. Different initiation.
    D10999 Genomic DNA. Translation: BAA01771.1.
    AL391753 Genomic DNA. Translation: CAC05822.1.
    AF348706 Genomic DNA. Translation: AAK18466.1.
    AF386526 Genomic DNA. Translation: AAL72333.1.
    M81458 Genomic DNA. No translation available.
    PIRiA44797.
    RefSeqiNP_085310.1. NC_002698.1.
    NP_858280.1. NC_004851.1.

    Genome annotation databases

    EnsemblBacteriaiAAL72333; AAL72333; CP0147.
    GeneIDi1238036.
    876517.
    KEGGisfl:CP0147.
    PATRICi18722607. VBIShiFle86970_5394.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91664 Genomic DNA. Translation: AAA26529.1 . Different initiation.
    D10999 Genomic DNA. Translation: BAA01771.1 .
    AL391753 Genomic DNA. Translation: CAC05822.1 .
    AF348706 Genomic DNA. Translation: AAK18466.1 .
    AF386526 Genomic DNA. Translation: AAL72333.1 .
    M81458 Genomic DNA. No translation available.
    PIRi A44797.
    RefSeqi NP_085310.1. NC_002698.1.
    NP_858280.1. NC_004851.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4A5P X-ray 3.15 A/B/C 318-686 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198214.CP0147.

    Proteomic databases

    PRIDEi P0A1I5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL72333 ; AAL72333 ; CP0147 .
    GeneIDi 1238036.
    876517.
    KEGGi sfl:CP0147.
    PATRICi 18722607. VBIShiFle86970_5394.

    Phylogenomic databases

    eggNOGi COG4789.
    HOGENOMi HOG000254015.
    KOi K03230.
    OMAi YNERVIS.
    OrthoDBi EOG6RZB0J.

    Family and domain databases

    InterProi IPR025505. FHIPEP_CS.
    IPR001712. T3SS_FHIPEP.
    IPR006302. T3SS_HrcV.
    [Graphical view ]
    Pfami PF00771. FHIPEP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005419. FlhA. 1 hit.
    PRINTSi PR00949. TYPE3IMAPROT.
    TIGRFAMsi TIGR01399. hrcV. 1 hit.
    PROSITEi PS00994. FHIPEP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "mxiA of Shigella flexneri 2a, which facilitates export of invasion plasmid antigens, encodes a homolog of the low-calcium-response protein, LcrD, of Yersinia pestis."
      Andrews G.P., Maurelli A.T.
      Infect. Immun. 60:3287-3295(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 700930 / 2457T / Serotype 2a.
      Plasmid: pWR100
    2. "A virulence locus, virH, on the large plasmid of Shigella flexneri."
      Sasakawa C.
      Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: YSH6000 / Serotype 2a.
      Plasmid: pMYSH6000
    3. "The virulence plasmid pWR100 and the repertoire of proteins secreted by the type III secretion apparatus of Shigella flexneri."
      Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H., Kunst F., Sansonetti P.J., Parsot C.
      Mol. Microbiol. 38:760-771(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: M90T / Serotype 5a.
      Plasmid: pWR100
    4. "Complete DNA sequence and analysis of the large virulence plasmid of Shigella flexneri."
      Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V., Blattner F.R.
      Infect. Immun. 69:3271-3285(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: M90T / Serotype 5a.
      Plasmid: pWR100
    5. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
      Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
      , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
      Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 301 / Serotype 2a.
      Plasmid: pCP301
    6. "Surface presentation of Shigella flexneri invasion plasmid antigens requires the products of the spa locus."
      Venkatesan M.M., Buysse J.M., Oaks E.V.
      J. Bacteriol. 174:1990-2001(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 489-686.
      Strain: M90T / Serotype 5a.
      Plasmid: pWR100
    7. "Eight genes in region 5 that form an operon are essential for invasion of epithelial cells by Shigella flexneri 2a."
      Sasakawa C., Komatsu K., Tobe T., Suzuki T., Yoshikawa M.
      J. Bacteriol. 175:2334-2346(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 489-686.
      Strain: YSH6000 / Serotype 2a.
      Plasmid: pMYSH6000

    Entry informationi

    Entry nameiMXIA_SHIFL
    AccessioniPrimary (citable) accession number: P0A1I5
    Secondary accession number(s): P35533, Q55295
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3