P0A1I5 (MXIA_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein MxiA

Alternative name(s):
Protein VirH

Gene names

Name:	mxiA
Synonyms:	virH
Ordered Locus Names:	CP0147

Encoded on

Plasmid pWR100 Ref.1 Ref.3 Ref.4 Ref.6
Plasmid pMYSH6000 Ref.2 Ref.7
Plasmid pCP301 Ref.5

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	686 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Necessary for the secretion of IPA invasins.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the FHIPEP (flagella/HR/invasion proteins export pore) family.
Sequence caution	The sequence AAA26529.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein transport Transport Virulence
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	3D-structure Complete proteome Plasmid
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW protein secretion Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 686

686

Protein MxiA

PRO_0000190032

Regions

Transmembrane

28 – 52

25

Helical; Potential

Transmembrane

105 – 129

25

Helical; Potential

Transmembrane

197 – 216

20

Helical; Potential

Transmembrane

232 – 256

25

Helical; Potential

Transmembrane

274 – 292

19

Helical; Potential

Transmembrane

299 – 315

17

Helical; Potential

Experimental info

Sequence conflict

95

1

A → R in AAA26529. Ref.1

Sequence conflict

255 – 256

2

IV → ML in AAA26529. Ref.1

Sequence conflict

342

1

S → T in AAA26529. Ref.1

Sequence conflict

355

1

A → R in AAA26529. Ref.1

Secondary structure

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686

Helix Strand Turn

Details...

Beta strand

362 – 368

7

Helix

377 – 391

15

Beta strand

400 – 407

8

Beta strand

409 – 416

8

Beta strand

419 – 425

7

Beta strand

430 – 433

4

Beta strand

448 – 452

5

Beta strand

455 – 461

7

Helix

478 – 491

14

Helix

494 – 496

3

Helix

500 – 511

12

Helix

515 – 522

8

Helix

527 – 538

12

Turn

539 – 541

3

Helix

547 – 557

11

Turn

558 – 560

3

Helix

564 – 574

11

Helix

576 – 583

8

Beta strand

588 – 594

7

Helix

596 – 604

9

Helix

623 – 637

15

Beta strand

638 – 640

3

Beta strand

644 – 647

4

Turn

649 – 651

3

Helix

652 – 660

9

Beta strand

664 – 666

3

Beta strand

668 – 670

3

Beta strand

679 – 686

8

Sequences

	Sequence		Length	Mass (Da)
	P0A1I5 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: BD675C2C3BED820F	FASTA	686	76,145
	10 20 30 40 50 60 MIQSFLKQVS TKPELIILVL MVMIIAMLII PLPTYLVDFL IGLNIVLAIL VFMGSFYIER 70 80 90 100 110 120 ILSFSTFPSV LLITTLFRLA LSISTSRLIL VDADAGKIIT TFGQFVIGDS LAVGFVIFSI 130 140 150 160 170 180 VTVVQFIVIT KGSERVAEVA ARFSLDGMPG KQMSIDADLK AGIIDAAGAK ERRSILERES 190 200 210 220 230 240 QLYGSFDGAM KFIKGDAIAG IIIIFVNLIG GISVGMSQHG MSLSGALSTY TILTIGDGLV 250 260 270 280 290 300 SQIPALLISI SAGFIVTRVN GDSDNMGRNI MSQIFGNPFV LIVTSALALA IGMLPGFPFF 310 320 330 340 350 360 VFFLIAVTLT ALFYYKKVVE KEKSLSESDS SGYTGTFDID NSHDSSLAMI ENLDAISSET 370 380 390 400 410 420 VPLILLFAEN KINANDMEGL IERIRSQFFI DYGVRLPTIL YRTSNELKVD DIVLLINEVR 430 440 450 460 470 480 ADSFNIYFDK VCITDENGDI DALGIPVVST SYNERVISWV DVSYTENLTN IDAKIKSAQD 490 500 510 520 530 540 EFYHQLSQAL LNNINEIFGI QETKNMLDQF ENRYPDLLKE VFRHVTIQRI SEVLQRLLGE 550 560 570 580 590 600 NISVRNLKLI MESLALWAPR EKDVITLVEH VRASLSRYIC SKIAVSGEIK VVMLSGYIED 610 620 630 640 650 660 AIRKGIRQTS GGSFLNMDIE VSDEVMETLA HALRELRNAK KNFVLLVSVD IRRFVKRLID 670 680 NRFKSILVIS YAEIDEAYTI NVLKTI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"mxiA of Shigella flexneri 2a, which facilitates export of invasion plasmid antigens, encodes a homolog of the low-calcium-response protein, LcrD, of Yersinia pestis." Andrews G.P., Maurelli A.T. Infect. Immun. 60:3287-3295(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.
[2]	"A virulence locus, virH, on the large plasmid of Shigella flexneri." Sasakawa C. Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YSH6000 / Serotype 2a.
[3]	"The virulence plasmid pWR100 and the repertoire of proteins secreted by the type III secretion apparatus of Shigella flexneri." Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H., Kunst F., Sansonetti P.J., Parsot C. Mol. Microbiol. 38:760-771(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: M90T / Serotype 5a.
[4]	"Complete DNA sequence and analysis of the large virulence plasmid of Shigella flexneri." Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V., Blattner F.R. Infect. Immun. 69:3271-3285(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: M90T / Serotype 5a.
[5]	"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.
[6]	"Surface presentation of Shigella flexneri invasion plasmid antigens requires the products of the spa locus." Venkatesan M.M., Buysse J.M., Oaks E.V. J. Bacteriol. 174:1990-2001(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 489-686. Strain: M90T / Serotype 5a.
[7]	"Eight genes in region 5 that form an operon are essential for invasion of epithelial cells by Shigella flexneri 2a." Sasakawa C., Komatsu K., Tobe T., Suzuki T., Yoshikawa M. J. Bacteriol. 175:2334-2346(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 489-686. Strain: YSH6000 / Serotype 2a.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M91664 Genomic DNA. Translation: AAA26529.1. Different initiation.
D10999 Genomic DNA. Translation: BAA01771.1.
AL391753 Genomic DNA. Translation: CAC05822.1.
AF348706 Genomic DNA. Translation: AAK18466.1.
AF386526 Genomic DNA. Translation: AAL72333.1.
M81458 Genomic DNA. No translation available.

PIR

A44797.

RefSeq

NP_085310.1. NC_002698.1.
NP_858280.1. NC_004851.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4A5P	X-ray	3.15	A/B/C	318-686	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

198214.CP0147.

Proteomic databases

PRIDE

P0A1I5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAL72333; AAL72333; CP0147.

GeneID

1238036.
876517.

KEGG

sfl:CP0147.

PATRIC

18722607. VBIShiFle86970_5394.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG4789.

HOGENOM

HOG000254015.

KO

K03230.

OMA

DEAYTIN.

ProtClustDB

PRK15337.

Family and domain databases

InterPro

IPR025505. FHIPEP_CS.
IPR001712. T3SS_FHIPEP.
IPR006302. T3SS_HrcV.
[Graphical view]

Pfam

PF00771. FHIPEP. 1 hit.
[Graphical view]

PIRSF

PIRSF005419. FlhA. 1 hit.

PRINTS

PR00949. TYPE3IMAPROT.

TIGRFAMs

TIGR01399. hrcV. 1 hit.

PROSITE

PS00994. FHIPEP. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

MXIA_SHIFL

Accession

Primary (citable) accession number: P0A1I5
Secondary accession number(s): P35533, Q55295

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program