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P0A1I5

- MXIA_SHIFL

UniProt

P0A1I5 - MXIA_SHIFL

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Protein
Protein MxiA
Gene
mxiA, virH, CP0147
Organism
Shigella flexneri
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Necessary for the secretion of IPA invasins.

GO - Biological processi

  1. pathogenesis Source: UniProtKB-KW
  2. protein secretion Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport, Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Protein MxiA
Alternative name(s):
Protein VirH
Gene namesi
Name:mxiA
Synonyms:virH
Ordered Locus Names:CP0147
Encoded oniPlasmid pWR1004 Publications
Plasmid pMYSH60002 Publications
Plasmid pCP3011 Publication
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000001006: Plasmid pCP301

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei28 – 5225Helical; Reviewed prediction
Add
BLAST
Transmembranei105 – 12925Helical; Reviewed prediction
Add
BLAST
Transmembranei197 – 21620Helical; Reviewed prediction
Add
BLAST
Transmembranei232 – 25625Helical; Reviewed prediction
Add
BLAST
Transmembranei274 – 29219Helical; Reviewed prediction
Add
BLAST
Transmembranei299 – 31517Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 686686Protein MxiA
PRO_0000190032Add
BLAST

Proteomic databases

PRIDEiP0A1I5.

Interactioni

Protein-protein interaction databases

STRINGi198214.CP0147.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi362 – 3687
Helixi377 – 39115
Beta strandi400 – 4078
Beta strandi409 – 4168
Beta strandi419 – 4257
Beta strandi430 – 4334
Beta strandi448 – 4525
Beta strandi455 – 4617
Helixi478 – 49114
Helixi494 – 4963
Helixi500 – 51112
Helixi515 – 5228
Helixi527 – 53812
Turni539 – 5413
Helixi547 – 55711
Turni558 – 5603
Helixi564 – 57411
Helixi576 – 5838
Beta strandi588 – 5947
Helixi596 – 6049
Helixi623 – 63715
Beta strandi638 – 6403
Beta strandi644 – 6474
Turni649 – 6513
Helixi652 – 6609
Beta strandi664 – 6663
Beta strandi668 – 6703
Beta strandi679 – 6868

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4A5PX-ray3.15A/B/C318-686[»]

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4789.
HOGENOMiHOG000254015.
KOiK03230.
OMAiYNERVIS.
OrthoDBiEOG6RZB0J.

Family and domain databases

InterProiIPR025505. FHIPEP_CS.
IPR001712. T3SS_FHIPEP.
IPR006302. T3SS_HrcV.
[Graphical view]
PfamiPF00771. FHIPEP. 1 hit.
[Graphical view]
PIRSFiPIRSF005419. FlhA. 1 hit.
PRINTSiPR00949. TYPE3IMAPROT.
TIGRFAMsiTIGR01399. hrcV. 1 hit.
PROSITEiPS00994. FHIPEP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A1I5-1 [UniParc]FASTAAdd to Basket

« Hide

MIQSFLKQVS TKPELIILVL MVMIIAMLII PLPTYLVDFL IGLNIVLAIL    50
VFMGSFYIER ILSFSTFPSV LLITTLFRLA LSISTSRLIL VDADAGKIIT 100
TFGQFVIGDS LAVGFVIFSI VTVVQFIVIT KGSERVAEVA ARFSLDGMPG 150
KQMSIDADLK AGIIDAAGAK ERRSILERES QLYGSFDGAM KFIKGDAIAG 200
IIIIFVNLIG GISVGMSQHG MSLSGALSTY TILTIGDGLV SQIPALLISI 250
SAGFIVTRVN GDSDNMGRNI MSQIFGNPFV LIVTSALALA IGMLPGFPFF 300
VFFLIAVTLT ALFYYKKVVE KEKSLSESDS SGYTGTFDID NSHDSSLAMI 350
ENLDAISSET VPLILLFAEN KINANDMEGL IERIRSQFFI DYGVRLPTIL 400
YRTSNELKVD DIVLLINEVR ADSFNIYFDK VCITDENGDI DALGIPVVST 450
SYNERVISWV DVSYTENLTN IDAKIKSAQD EFYHQLSQAL LNNINEIFGI 500
QETKNMLDQF ENRYPDLLKE VFRHVTIQRI SEVLQRLLGE NISVRNLKLI 550
MESLALWAPR EKDVITLVEH VRASLSRYIC SKIAVSGEIK VVMLSGYIED 600
AIRKGIRQTS GGSFLNMDIE VSDEVMETLA HALRELRNAK KNFVLLVSVD 650
IRRFVKRLID NRFKSILVIS YAEIDEAYTI NVLKTI 686
Length:686
Mass (Da):76,145
Last modified:March 1, 2005 - v1
Checksum:iBD675C2C3BED820F
GO

Sequence cautioni

The sequence AAA26529.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti95 – 951A → R in AAA26529. 1 Publication
Sequence conflicti255 – 2562IV → ML in AAA26529. 1 Publication
Sequence conflicti342 – 3421S → T in AAA26529. 1 Publication
Sequence conflicti355 – 3551A → R in AAA26529. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M91664 Genomic DNA. Translation: AAA26529.1. Different initiation.
D10999 Genomic DNA. Translation: BAA01771.1.
AL391753 Genomic DNA. Translation: CAC05822.1.
AF348706 Genomic DNA. Translation: AAK18466.1.
AF386526 Genomic DNA. Translation: AAL72333.1.
M81458 Genomic DNA. No translation available.
PIRiA44797.
RefSeqiNP_085310.1. NC_002698.1.
NP_858280.1. NC_004851.1.

Genome annotation databases

EnsemblBacteriaiAAL72333; AAL72333; CP0147.
GeneIDi1238036.
876517.
KEGGisfl:CP0147.
PATRICi18722607. VBIShiFle86970_5394.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M91664 Genomic DNA. Translation: AAA26529.1 . Different initiation.
D10999 Genomic DNA. Translation: BAA01771.1 .
AL391753 Genomic DNA. Translation: CAC05822.1 .
AF348706 Genomic DNA. Translation: AAK18466.1 .
AF386526 Genomic DNA. Translation: AAL72333.1 .
M81458 Genomic DNA. No translation available.
PIRi A44797.
RefSeqi NP_085310.1. NC_002698.1.
NP_858280.1. NC_004851.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4A5P X-ray 3.15 A/B/C 318-686 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 198214.CP0147.

Proteomic databases

PRIDEi P0A1I5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL72333 ; AAL72333 ; CP0147 .
GeneIDi 1238036.
876517.
KEGGi sfl:CP0147.
PATRICi 18722607. VBIShiFle86970_5394.

Phylogenomic databases

eggNOGi COG4789.
HOGENOMi HOG000254015.
KOi K03230.
OMAi YNERVIS.
OrthoDBi EOG6RZB0J.

Family and domain databases

InterProi IPR025505. FHIPEP_CS.
IPR001712. T3SS_FHIPEP.
IPR006302. T3SS_HrcV.
[Graphical view ]
Pfami PF00771. FHIPEP. 1 hit.
[Graphical view ]
PIRSFi PIRSF005419. FlhA. 1 hit.
PRINTSi PR00949. TYPE3IMAPROT.
TIGRFAMsi TIGR01399. hrcV. 1 hit.
PROSITEi PS00994. FHIPEP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "mxiA of Shigella flexneri 2a, which facilitates export of invasion plasmid antigens, encodes a homolog of the low-calcium-response protein, LcrD, of Yersinia pestis."
    Andrews G.P., Maurelli A.T.
    Infect. Immun. 60:3287-3295(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.
    Plasmid: pWR100
  2. "A virulence locus, virH, on the large plasmid of Shigella flexneri."
    Sasakawa C.
    Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YSH6000 / Serotype 2a.
    Plasmid: pMYSH6000
  3. "The virulence plasmid pWR100 and the repertoire of proteins secreted by the type III secretion apparatus of Shigella flexneri."
    Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H., Kunst F., Sansonetti P.J., Parsot C.
    Mol. Microbiol. 38:760-771(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: M90T / Serotype 5a.
    Plasmid: pWR100
  4. "Complete DNA sequence and analysis of the large virulence plasmid of Shigella flexneri."
    Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V., Blattner F.R.
    Infect. Immun. 69:3271-3285(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: M90T / Serotype 5a.
    Plasmid: pWR100
  5. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
    Plasmid: pCP301
  6. "Surface presentation of Shigella flexneri invasion plasmid antigens requires the products of the spa locus."
    Venkatesan M.M., Buysse J.M., Oaks E.V.
    J. Bacteriol. 174:1990-2001(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 489-686.
    Strain: M90T / Serotype 5a.
    Plasmid: pWR100
  7. "Eight genes in region 5 that form an operon are essential for invasion of epithelial cells by Shigella flexneri 2a."
    Sasakawa C., Komatsu K., Tobe T., Suzuki T., Yoshikawa M.
    J. Bacteriol. 175:2334-2346(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 489-686.
    Strain: YSH6000 / Serotype 2a.
    Plasmid: pMYSH6000

Entry informationi

Entry nameiMXIA_SHIFL
AccessioniPrimary (citable) accession number: P0A1I5
Secondary accession number(s): P35533, Q55295
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: May 14, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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