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P0A1I5 (MXIA_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein MxiA
Alternative name(s):
Protein VirH
Gene names
Name:mxiA
Synonyms:virH
Ordered Locus Names:CP0147
Encoded onPlasmid pWR100 Ref.1 Ref.3 Ref.4 Ref.6
Plasmid pMYSH6000 Ref.2 Ref.7
Plasmid pCP301 Ref.5
OrganismShigella flexneri [Complete proteome] [HAMAP]
Taxonomic identifier623 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella

Protein attributes

Sequence length686 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Necessary for the secretion of IPA invasins.

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the FHIPEP (flagella/HR/invasion proteins export pore) family.

Sequence caution

The sequence AAA26529.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 686686Protein MxiA
PRO_0000190032

Regions

Transmembrane28 – 5225Helical; Potential
Transmembrane105 – 12925Helical; Potential
Transmembrane197 – 21620Helical; Potential
Transmembrane232 – 25625Helical; Potential
Transmembrane274 – 29219Helical; Potential
Transmembrane299 – 31517Helical; Potential

Experimental info

Sequence conflict951A → R in AAA26529. Ref.1
Sequence conflict255 – 2562IV → ML in AAA26529. Ref.1
Sequence conflict3421S → T in AAA26529. Ref.1
Sequence conflict3551A → R in AAA26529. Ref.1

Secondary structure

.................................................... 686
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A1I5 [UniParc].

Last modified March 1, 2005. Version 1.
Checksum: BD675C2C3BED820F

FASTA68676,145
        10         20         30         40         50         60 
MIQSFLKQVS TKPELIILVL MVMIIAMLII PLPTYLVDFL IGLNIVLAIL VFMGSFYIER 

        70         80         90        100        110        120 
ILSFSTFPSV LLITTLFRLA LSISTSRLIL VDADAGKIIT TFGQFVIGDS LAVGFVIFSI 

       130        140        150        160        170        180 
VTVVQFIVIT KGSERVAEVA ARFSLDGMPG KQMSIDADLK AGIIDAAGAK ERRSILERES 

       190        200        210        220        230        240 
QLYGSFDGAM KFIKGDAIAG IIIIFVNLIG GISVGMSQHG MSLSGALSTY TILTIGDGLV 

       250        260        270        280        290        300 
SQIPALLISI SAGFIVTRVN GDSDNMGRNI MSQIFGNPFV LIVTSALALA IGMLPGFPFF 

       310        320        330        340        350        360 
VFFLIAVTLT ALFYYKKVVE KEKSLSESDS SGYTGTFDID NSHDSSLAMI ENLDAISSET 

       370        380        390        400        410        420 
VPLILLFAEN KINANDMEGL IERIRSQFFI DYGVRLPTIL YRTSNELKVD DIVLLINEVR 

       430        440        450        460        470        480 
ADSFNIYFDK VCITDENGDI DALGIPVVST SYNERVISWV DVSYTENLTN IDAKIKSAQD 

       490        500        510        520        530        540 
EFYHQLSQAL LNNINEIFGI QETKNMLDQF ENRYPDLLKE VFRHVTIQRI SEVLQRLLGE 

       550        560        570        580        590        600 
NISVRNLKLI MESLALWAPR EKDVITLVEH VRASLSRYIC SKIAVSGEIK VVMLSGYIED 

       610        620        630        640        650        660 
AIRKGIRQTS GGSFLNMDIE VSDEVMETLA HALRELRNAK KNFVLLVSVD IRRFVKRLID 

       670        680 
NRFKSILVIS YAEIDEAYTI NVLKTI 

« Hide

References

« Hide 'large scale' references
[1]"mxiA of Shigella flexneri 2a, which facilitates export of invasion plasmid antigens, encodes a homolog of the low-calcium-response protein, LcrD, of Yersinia pestis."
Andrews G.P., Maurelli A.T.
Infect. Immun. 60:3287-3295(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 700930 / 2457T / Serotype 2a.
[2]"A virulence locus, virH, on the large plasmid of Shigella flexneri."
Sasakawa C.
Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: YSH6000 / Serotype 2a.
[3]"The virulence plasmid pWR100 and the repertoire of proteins secreted by the type III secretion apparatus of Shigella flexneri."
Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H., Kunst F., Sansonetti P.J., Parsot C.
Mol. Microbiol. 38:760-771(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: M90T / Serotype 5a.
[4]"Complete DNA sequence and analysis of the large virulence plasmid of Shigella flexneri."
Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V., Blattner F.R.
Infect. Immun. 69:3271-3285(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: M90T / Serotype 5a.
[5]"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. expand/collapse author list , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 301 / Serotype 2a.
[6]"Surface presentation of Shigella flexneri invasion plasmid antigens requires the products of the spa locus."
Venkatesan M.M., Buysse J.M., Oaks E.V.
J. Bacteriol. 174:1990-2001(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 489-686.
Strain: M90T / Serotype 5a.
[7]"Eight genes in region 5 that form an operon are essential for invasion of epithelial cells by Shigella flexneri 2a."
Sasakawa C., Komatsu K., Tobe T., Suzuki T., Yoshikawa M.
J. Bacteriol. 175:2334-2346(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 489-686.
Strain: YSH6000 / Serotype 2a.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M91664 Genomic DNA. Translation: AAA26529.1. Different initiation.
D10999 Genomic DNA. Translation: BAA01771.1.
AL391753 Genomic DNA. Translation: CAC05822.1.
AF348706 Genomic DNA. Translation: AAK18466.1.
AF386526 Genomic DNA. Translation: AAL72333.1.
M81458 Genomic DNA. No translation available.
PIRA44797.
RefSeqNP_085310.1. NC_002698.1.
NP_858280.1. NC_004851.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4A5PX-ray3.15A/B/C318-686[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198214.CP0147.

Proteomic databases

PRIDEP0A1I5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL72333; AAL72333; CP0147.
GeneID1238036.
876517.
KEGGsfl:CP0147.
PATRIC18722607. VBIShiFle86970_5394.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG4789.
HOGENOMHOG000254015.
KOK03230.
OMAYNERVIS.
OrthoDBEOG6RZB0J.

Family and domain databases

InterProIPR025505. FHIPEP_CS.
IPR001712. T3SS_FHIPEP.
IPR006302. T3SS_HrcV.
[Graphical view]
PfamPF00771. FHIPEP. 1 hit.
[Graphical view]
PIRSFPIRSF005419. FlhA. 1 hit.
PRINTSPR00949. TYPE3IMAPROT.
TIGRFAMsTIGR01399. hrcV. 1 hit.
PROSITEPS00994. FHIPEP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMXIA_SHIFL
AccessionPrimary (citable) accession number: P0A1I5
Secondary accession number(s): P35533, Q55295
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: May 14, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references