Reviewed, UniProtKB/Swiss-Prot P0A1F8 (DHAS_SALTY)

Names and origin

Protein names

Recommended name:
    Aspartate-semialdehyde dehydrogenase
      Short name=ASA dehydrogenase
      Short name=ASADH
    EC=1.2.1.11

Gene names

Name:	asd
Ordered Locus Names:	STM3539

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 2/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the aspartate-semialdehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro aspartate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Aspartate-semialdehyde dehydrogenase		PRO_0000141373
Sites
	Active site	136	1	Acyl-thioester intermediate By similarity
	Binding site	136	1	Cysteine (covalent); in inhibited form By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A1F8-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 08BFB3E98029A677	FASTA	368	40,136
	10 20 30 40 50 60 MKNVGFIGWR GMVGSVLMQR MVEERDFDAI RPVFFSTSQF GQAAPTFGDT STGTLQDAFD 70 80 90 100 110 120 LDALKALDII VTCQGGDYTN EIYPKLRESG WQGYWIDAAS TLRMKDDAII ILDPVNQDVI 130 140 150 160 170 180 TDGLNNGVKT FVGGNCTVSL MLMSLGGLFA HNLVDWVSVA TYQAASGGGA RHMRELLTQM 190 200 210 220 230 240 GQLYGHVADE LATPSSAILD IERKVTALTR SGELPVDNFG VPLAGSLIPW IDKQLDNGQS 250 260 270 280 290 300 REEWKGQAET NKILNTASVI PVDGLCVRVG ALRCHSQAFT IKLKKEVSIP TVEELLAAHN 310 320 330 340 350 360 PWAKVVPNDR DITMRELTPA AVTGTLTTPV GRLRKLNMGP EFLSAFTVGD QLLWGAAEPL RRMLRQLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Galan J., Antoine G., Curtiss R. III Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF015781 Genomic DNA. Translation: AAB69392.1. Different initiation. AE008863 Genomic DNA. Translation: AAL22399.1.
RefSeq	NP_462440.1.
3D structure databases
HSSP	HSSP built from PDB template 1BRM based on UniProtKB P00353.
SMR	P0A1F8. Positions 1-368.
ModBase	Search...
Genome annotation databases
GeneID	1255062.
GenomeReviews	Gene locus STM3539 in contig AE006468_GR.
KEGG	stm:STM3539.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A1F8.
OMA	P0A1F8. IDGLCVR.
Enzyme and pathway databases
BioCyc	STYP99287:STM3539-MON.
BRENDA	1.2.1.11. 2.
Family and domain databases
InterPro	IPR000319. Asp-semialdehyde_DH_CS. IPR011534. Asp_ADH_proteob. IPR012080. Asp_semialdehyde_DH. IPR016040. NAD(P)-bd_dom. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
PIRSF	PIRSF000148. ASA_dh. 1 hit.
TIGRFAMs	TIGR01745. asd_gamma. 1 hit.
PROSITE	PS01103. ASD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHAS_SALTY

Accession

Primary (citable) accession number: P0A1F8
Secondary accession number(s): O30706

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)