P0A1F6 (UDP_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uridine phosphorylase
Short name=UPase
Short name=UrdPase
EC=2.4.2.3

Gene names

Name:	udp
Ordered Locus Names:	STM3968
ORF Names:	STMD1.21

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis By similarity.
Catalytic activity	Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate.
Pathway	Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from uridine (phosphorylase route): step 1/1.
Subunit structure	Homohexamer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PNP/UDP phosphorylase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro nucleotide catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	uridine phosphorylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 253

252

Uridine phosphorylase

PRO_0000063185

Experimental info

Sequence conflict

200

1

A → R in CAA73795. Ref.2

Secondary structure

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253

Helix Strand Turn

Details...

Turn

7 – 9

3

Helix

13 – 16

4

Beta strand

21 – 23

3

Helix

28 – 30

3

Helix

31 – 35

5

Beta strand

38 – 47

10

Beta strand

50 – 57

8

Beta strand

60 – 65

6

Helix

71 – 83

13

Beta strand

88 – 97

10

Beta strand

107 – 118

12

Helix

119 – 122

4

Helix

134 – 147

14

Beta strand

151 – 160

10

Helix

164 – 166

3

Beta strand

171 – 173

3

Helix

178 – 180

3

Helix

183 – 189

7

Beta strand

194 – 198

5

Helix

199 – 207

9

Turn

208 – 210

3

Beta strand

212 – 222

11

Turn

231 – 233

3

Helix

237 – 250

14

Sequences

	Sequence		Length	Mass (Da)
	P0A1F6 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 63F26AFB8427BED2	FASTA	253	27,139
	10 20 30 40 50 60 MSKSDVFHLG LTKNDLQGAQ LAIVPGDPER VEKIAALMDK PVKLASHREF TSWRAELDGK 70 80 90 100 110 120 AVIVCSTGIG GPSTSIAVEE LAQLGIRTFL RIGTTGAIQP HINVGDVLVT TASVRLDGAS 130 140 150 160 170 180 LHFAPMEFPA VADFACTTAL VEAAKSIGAT THVGVTASSD TFYPGQERYD TYSGRVVRRF 190 200 210 220 230 240 KGSMEEWQAM GVMNYEMESA TLLTMCASQG LRAGMVAGVI VNRTQQEIPN AETMKQTESH 250 AVKIVVEAAR RLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of the gene encoding uridine phosphorylase (udp) in Salmonella typhimurium." Errais L.L., Ukhabotina L.S., Eremina S.Y., Evdokimova A.A., Mironov A.S. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Protein engineering of uridine phosphorylase from Escherichia coli K-12. I. Cloning and expression of uridine phosphorylase genes from Klebsiella aerogenes and Salmonella typhimurium in E. coli." Veiko V.P., Chebotaev D.V., Ovcharova I.V., Gul'Ko L.B. Bioorg. Khim. 24:381-387(1998) [PubMed: 9661793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[3]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y14282 Genomic DNA. Translation: CAA74658.2.
Y13360 Genomic DNA. Translation: CAA73795.1.
AF233324 Genomic DNA. Translation: AAF33424.1.
AE006468 Genomic DNA. Translation: AAL22812.1.

RefSeq

NP_462853.1. NC_003197.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RYZ	X-ray	2.90	A/B/C/D/E/F	1-253	[»]
1SJ9	X-ray	2.50	A/B/C/D/E/F	1-253	[»]
1Y1Q	X-ray	2.35	A/B/C/D/E/F	1-253	[»]
1Y1R	X-ray	2.11	A/B/C/D/E/F	1-253	[»]
1Y1S	X-ray	2.55	A/B/C/D/E/F	1-253	[»]
1Y1T	X-ray	1.77	A/F	1-253	[»]
1ZL2	X-ray	1.85	A/B/C/D/E/F	1-253	[»]
2HN9	X-ray	2.12	A/B/C/D/E/F	1-253	[»]
2HRD	X-ray	1.70	A/B/C/D/E/F	1-253	[»]
2HSW	X-ray	1.99	A/B	1-253	[»]
2HWU	X-ray	2.91	A/B/C/D/E/F	1-253	[»]
2I8A	X-ray	1.64	A/B/C/D/E/F	2-252	[»]
2IQ5	X-ray	1.90	A/B	2-252	[»]
2OEC	X-ray	2.19	A/B/C/D/E/F	2-252	[»]
2OXF	X-ray	1.76	A/F	4-253	[»]
2PGA	X-ray	1.74	A/B/C/D/E/F	1-253	[»]
2QDK	X-ray	1.62	A/B/C/D/E/F	2-253	[»]
2RJ3	X-ray	2.51	A/B/C/D/E/F	2-253	[»]
3C74	X-ray	2.38	A/B/C/D/E/F	2-253	[»]
3DDO	X-ray	1.50	A/B/C/D/E/F	1-253	[»]
3DPS	X-ray	1.80	A/F	1-253	[»]
3FWP	X-ray	1.86	A/B/C/D/E/F	1-253	[»]

ProteinModelPortal

P0A1F6.

SMR

P0A1F6. Positions 1-253.

ModBase

Search...

Proteomic databases

PRIDE

P0A1F6.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1255494.

GenomeReviews

Gene locus STM3968 in contig AE006468_GR.

KEGG

stm:STM3968.

PATRIC

32386803. VBISalEnt20916_4187.

Phylogenomic databases

HOGENOM

HBG617197.

OMA

STREFTS.

ProtClustDB

PRK11178.

Enzyme and pathway databases

BioCyc

STYP99287:STM3968-MONOMER.

Family and domain databases

InterPro

IPR018017. Nucleoside_phosphorylase.
IPR018016. Nucleoside_phosphorylase_CS.
IPR000845. Nucleoside_phosphorylase_d.
IPR010058. Uridine_phosphorylase.
[Graphical view]

KO

K00757.

PANTHER

PTHR21234. PNP_UDP. 1 hit.

Pfam

PF01048. PNP_UDP_1. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01718. Uridine-psphlse. 1 hit.

PROSITE

PS01232. PNP_UDP_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

UDP_SALTY

Accession

Primary (citable) accession number: P0A1F6
Secondary accession number(s): O08432, O33808, Q9L6M8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program