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Protein

Cysteine synthase A

Gene

cysK

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Two cysteine synthase enzymes are found, this enzyme and CysM; both catalyze the same reaction. Cysteine synthase B (CysM) can also use thiosulfate in place of sulfide to give cysteine thiosulfonate as a product.1 Publication

Catalytic activityi

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.1 Publication

Cofactori

pyridoxal 5'-phosphate2 Publications

Enzyme regulationi

O-acetyl-L-serine causes the CysE-CysK complex to dissociate in the absence of hydrogen sulfide.1 Publication

Kineticsi

  1. KM=5 mM for O-acetyl-L-serine as isolated subunit and in complex with CysK1 Publication

    Pathwayi: L-cysteine biosynthesis

    This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-serine.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Serine acetyltransferase (cysE)
    2. Cysteine synthase A (cysK), Cysteine synthase B (cysM)
    This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei8Allosteric inhibitor1 Publication1
    Binding sitei72Pyridoxal phosphate3 Publications1
    Binding sitei269Allosteric inhibitor; via amide nitrogen1 Publication1
    Binding sitei273Pyridoxal phosphate3 Publications1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Cysteine biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BRENDAi2.5.1.47. 2169.
    SABIO-RKP0A1E3.
    UniPathwayiUPA00136; UER00200.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine synthase A (EC:2.5.1.471 Publication)
    Short name:
    CSase A
    Alternative name(s):
    O-acetylserine (thiol)-lyase A1 Publication
    Short name:
    OAS-TL A
    O-acetylserine sulfhydrylase A1 Publication
    Gene namesi
    Name:cysK
    Ordered Locus Names:STM2430
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
    Proteomesi
    • UP000001014 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00001670892 – 323Cysteine synthase AAdd BLAST322

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei42N6-(pyridoxal phosphate)lysine1

    Proteomic databases

    PaxDbiP0A1E3.
    PRIDEiP0A1E3.

    Interactioni

    Subunit structurei

    Part of the cysteine synthase complex formed at a ratio of 2 copies of this protein and 1 copy of serine acetyltransferase (cysE). The complex reversibly dissociates in the presence of O-acetyl-L-serine but in the absence of hydrogen sulfide (PubMed:4977445). Homodimer (PubMed:10452898, PubMed:11023792, PubMed:9761678).4 Publications

    Protein-protein interaction databases

    STRINGi99287.STM2430.

    Structurei

    Secondary structure

    1323
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi4 – 7Combined sources4
    Helixi8 – 11Combined sources4
    Beta strandi17 – 19Combined sources3
    Beta strandi21 – 26Combined sources6
    Beta strandi28 – 32Combined sources5
    Helixi37 – 39Combined sources3
    Helixi42 – 55Combined sources14
    Beta strandi64 – 68Combined sources5
    Helixi72 – 84Combined sources13
    Beta strandi88 – 93Combined sources6
    Helixi98 – 106Combined sources9
    Beta strandi110 – 114Combined sources5
    Helixi116 – 118Combined sources3
    Helixi119 – 132Combined sources14
    Turni135 – 137Combined sources3
    Beta strandi138 – 140Combined sources3
    Turni143 – 145Combined sources3
    Helixi148 – 155Combined sources8
    Helixi157 – 164Combined sources8
    Turni165 – 167Combined sources3
    Beta strandi171 – 175Combined sources5
    Beta strandi177 – 179Combined sources3
    Helixi180 – 190Combined sources11
    Turni191 – 193Combined sources3
    Beta strandi199 – 205Combined sources7
    Helixi210 – 215Combined sources6
    Helixi241 – 243Combined sources3
    Beta strandi245 – 250Combined sources6
    Helixi252 – 266Combined sources15
    Helixi272 – 284Combined sources13
    Helixi288 – 290Combined sources3
    Beta strandi295 – 299Combined sources5
    Helixi303 – 306Combined sources4
    Helixi310 – 312Combined sources3
    Helixi318 – 321Combined sources4

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1D6SX-ray2.30A/B2-323[»]
    1FCJX-ray2.00A/B/C/D2-323[»]
    1OASX-ray2.20A/B2-323[»]
    ProteinModelPortaliP0A1E3.
    SMRiP0A1E3.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A1E3.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni177 – 181Pyridoxal phosphate binding5

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105C6T. Bacteria.
    COG0031. LUCA.
    HOGENOMiHOG000217394.
    KOiK01738.
    OMAiVKCRIGS.
    PhylomeDBiP0A1E3.

    Family and domain databases

    InterProiIPR005856. Cys_synth.
    IPR005859. CysK.
    IPR001216. P-phosphate_BS.
    IPR001926. TrpB-like_PLP-dep.
    [Graphical view]
    PfamiPF00291. PALP. 1 hit.
    [Graphical view]
    SUPFAMiSSF53686. SSF53686. 1 hit.
    TIGRFAMsiTIGR01139. cysK. 1 hit.
    TIGR01136. cysKM. 1 hit.
    PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0A1E3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSKIYEDNSL TIGHTPLVRL NRIGNGRILA KVESRNPSFS VKCRIGANMI
    60 70 80 90 100
    WDAEKRGVLK PGVELVEPTS GNTGIALAYV AAARGYKLTL TMPETMSIER
    110 120 130 140 150
    RKLLKALGAN LVLTEGAKGM KGAIQKAEEI VASDPQKYLL LQQFSNPANP
    160 170 180 190 200
    EIHEKTTGPE IWEDTDGQVD VFISGVGTGG TLTGVTRYIK GTKGKTDLIT
    210 220 230 240 250
    VAVEPTDSPV IAQALAGEEI KPGPHKIQGI GAGFIPGNLD LKLIDKVVGI
    260 270 280 290 300
    TNEEAISTAR RLMEEEGILA GISSGAAVAA ALKLQEDESF TNKNIVVILP
    310 320
    SSGERYLSTA LFADLFTEKE LQQ
    Length:323
    Mass (Da):34,536
    Last modified:January 23, 2007 - v2
    Checksum:iCE74168FAAE99B9E
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti70S → N in AAA27051 (PubMed:3290198).Curated1
    Sequence conflicti267 – 268GI → VF in AAA27051 (PubMed:3290198).Curated2

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M21450 Genomic DNA. Translation: AAA27051.1.
    AE006468 Genomic DNA. Translation: AAL21324.1.
    PIRiB28181. SYEBAC.
    RefSeqiNP_461365.1. NC_003197.1.
    WP_000036904.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL21324; AAL21324; STM2430.
    GeneIDi1253952.
    KEGGistm:STM2430.
    PATRICi32383489. VBISalEnt20916_2567.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M21450 Genomic DNA. Translation: AAA27051.1.
    AE006468 Genomic DNA. Translation: AAL21324.1.
    PIRiB28181. SYEBAC.
    RefSeqiNP_461365.1. NC_003197.1.
    WP_000036904.1. NC_003197.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1D6SX-ray2.30A/B2-323[»]
    1FCJX-ray2.00A/B/C/D2-323[»]
    1OASX-ray2.20A/B2-323[»]
    ProteinModelPortaliP0A1E3.
    SMRiP0A1E3.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi99287.STM2430.

    Proteomic databases

    PaxDbiP0A1E3.
    PRIDEiP0A1E3.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAL21324; AAL21324; STM2430.
    GeneIDi1253952.
    KEGGistm:STM2430.
    PATRICi32383489. VBISalEnt20916_2567.

    Phylogenomic databases

    eggNOGiENOG4105C6T. Bacteria.
    COG0031. LUCA.
    HOGENOMiHOG000217394.
    KOiK01738.
    OMAiVKCRIGS.
    PhylomeDBiP0A1E3.

    Enzyme and pathway databases

    UniPathwayiUPA00136; UER00200.
    BRENDAi2.5.1.47. 2169.
    SABIO-RKP0A1E3.

    Miscellaneous databases

    EvolutionaryTraceiP0A1E3.

    Family and domain databases

    InterProiIPR005856. Cys_synth.
    IPR005859. CysK.
    IPR001216. P-phosphate_BS.
    IPR001926. TrpB-like_PLP-dep.
    [Graphical view]
    PfamiPF00291. PALP. 1 hit.
    [Graphical view]
    SUPFAMiSSF53686. SSF53686. 1 hit.
    TIGRFAMsiTIGR01139. cysK. 1 hit.
    TIGR01136. cysKM. 1 hit.
    PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCYSK_SALTY
    AccessioniPrimary (citable) accession number: P0A1E3
    Secondary accession number(s): P12674
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: January 23, 2007
    Last modified: November 2, 2016
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.