Reviewed, UniProtKB/Swiss-Prot P0A1E2 (HEMN_SALTI)

Names and origin

Protein names

Recommended name:
    Oxygen-independent coproporphyrinogen-III oxidase
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.99.22

Gene names

Name:	hemN
Ordered Locus Names:	STY3877, t3617

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX By similarity.
Catalytic activity	Coproporphyrinogen-III + 2 S-adenosyl-L-methionine = protoporphyrinogen-IX + 2 CO2 + 2 L-methionine + 2 5'-deoxyadenosine.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (AdoMet route): step 1/1.
Subunit structure	Monomer.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the anaerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW coproporphyrinogen dehydrogenase activity Inferred from electronic annotation. Source: EC coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Oxygen-independent coproporphyrinogen-III oxidase		PRO_0000109951
Regions
	Region	113 – 114	2	S-adenosyl-L-methionine 2 binding By similarity
Sites
	Metal binding	62	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	66	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	69	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Binding site	56	1	S-adenosyl-L-methionine 1 By similarity
	Binding site	68	1	S-adenosyl-L-methionine 2; via carbonyl oxygen By similarity
	Binding site	112	1	S-adenosyl-L-methionine 1; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	145	1	S-adenosyl-L-methionine 1 By similarity
	Binding site	172	1	S-adenosyl-L-methionine 2 By similarity
	Binding site	184	1	S-adenosyl-L-methionine 2 By similarity
	Binding site	209	1	S-adenosyl-L-methionine 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A1E2-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 5667B4FE76204DAB	FASTA	457	52,828
	10 20 30 40 50 60 MSEQQIDWDL ALIQKYNYSG PRYTSYPTAL EFSEDFEDAA FLQAVARYPE RPLSLYVHIP 70 80 90 100 110 120 FCHKLCYFCG CNKIVTRQQH KADQYLDALE QEIRHRAPLF ADRHVSQLHW GGGTPTYLNK 130 140 150 160 170 180 AQISRLMTLL RENFHFNTDA EISIEVDPRE IELDVLDHLR AEGFNRLSMG VQDFNKEVQR 190 200 210 220 230 240 LVNREQDEEF IFALLNHARD IGFTSTNIDL IYGLPKQTPE SFAFTLKRVT ELNPDRLSVF 250 260 270 280 290 300 NYAHLPTLFA AQRKIKDADL PSAQQKLDIL QETIVSLTQA GYQFIGMDHF ARPDDELAVA 310 320 330 340 350 360 QREGVLHRNF QGYTTQGDTD LLGMGVSAIS MIGDGYMQNQ KELKRYYQQV DERGNALWRG 370 380 390 400 410 420 ITLTRDDCIR RDVIKALICN FRLDFNAVEQ QWGLHFAEYF AEDLQLLSPL AKDGLVDISE 430 440 450 KGIQVTAKGR LLIRNICMCF DAYLRQKARM QQFSRVI

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL627280 Genomic DNA. Translation: CAD03096.1. AE014613 Genomic DNA. Translation: AAO71118.1.
RefSeq	NP_458045.1. NP_807258.1.
3D structure databases
SMR	P0A1E2. Positions 4-444.
ModBase	Search...
Proteomic databases
PRIDE	P0A1E2.
Genome annotation databases
GeneID	1068278. 1250123.
GenomeReviews	Gene locus t3617 in contig AE014613_GR.
KEGG	stt:t3617. sty:STY3877.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285973.
OMA	HLPSRFA.
Enzyme and pathway databases
BioCyc	SENT209261:T3617-MONOMER. SENT220341:STY3877-MONOMER.
BRENDA	1.3.99.22. 3716.
Family and domain databases
InterPro	IPR006638. Elp3/MiaB/NifB. IPR004558. HemN. IPR010723. HemN_C. IPR007197. Radical_SAM. [Graphical view]
Pfam	PF06969. HemN_C. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00538. hemN. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HEMN_SALTI

Accession

Primary (citable) accession number: P0A1E2
Secondary accession number(s): P37129

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)