P0A1B5 (AROF_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive
EC=2.5.1.54

Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase

Gene names

Name:	aroF
Ordered Locus Names:	STM2670

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).
Catalytic activity	Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.
Miscellaneous	There are 3 DAHP synthases, AroF is feedback-inhibited by Tyr. The other 2 DAHP synthases are Phe- and Trp-sensitive, respectively.
Sequence similarities	Belongs to the class-I DAHP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-deoxy-7-phosphoheptulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive		PRO_0000140834
Experimental info
	Sequence conflict	185	1	G → A in AAA27030. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0A1B5 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 51EC218FC47B30A4	FASTA	356	38,713
	10 20 30 40 50 60 MQKDALNNVR ITDEQVLMTP EQLKAAFPLS LAQEAQIAQS RGIISDIIAG RDPRLLVVCG 70 80 90 100 110 120 PCSIHDPETA LEYARRFKAL AAEVSDSLYL VMRVYFEKPR TTVGWKGLIN DPHMDGSFDV 130 140 150 160 170 180 EAGLKIARQL LVELVNMGLP LATEALDPNS PQYLGDLFSW SAIGARTTES QTHREMASGL 190 200 210 220 230 240 SMPVGFKNGT DGSLATAINA MRAAAQPHRF VGINQAGQVA LLQTQGNPHG HVILRGGKAP 250 260 270 280 290 300 NYSPADVAQC EKEMEQAGLR PSLMVDCSHG NSNKDYRRQP AVAESVVAQI KDGNRSIIGL 310 320 330 340 350 MIESNIHEGN QSSEQPRSEM KYGVSVTDAC ISWEMTDALL REIHKDLSGQ LAVRVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of the Salmonella typhimurium aroF gene in Escherichia coli." Muday G.K., Herrmann K.M. J. Bacteriol. 172:2259-2266(1990) [PubMed: 1970560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M31302 Genomic DNA. Translation: AAA27030.1. AE006468 Genomic DNA. Translation: AAL21559.1.
PIR	A35253.
RefSeq	NP_461600.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P0A1B5.
SMR	P0A1B5. Positions 8-348.
ModBase	Search...
Proteomic databases
PRIDE	P0A1B5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1254193.
GenomeReviews	Gene locus STM2670 in contig AE006468_GR.
KEGG	stm:STM2670.
PATRIC	32383993. VBISalEnt20916_2814.
Phylogenomic databases
HOGENOM	HBG670536.
OMA	RGNRYGH.
ProtClustDB	PRK12755.
Enzyme and pathway databases
BioCyc	STYP99287:STM2670-MONOMER.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006219. DHAP_synth_1. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01626.
PANTHER	PTHR21225. AroFGH. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
PIRSF	PIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMs	TIGR00034. AroFGH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROF_SALTY

Accession

Primary (citable) accession number: P0A1B5
Secondary accession number(s): P21307

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program