Reviewed, UniProtKB/Swiss-Prot P0A1B0 (EX3_SALTI)

Names and origin

Protein names

Recommended name:
    Exodeoxyribonuclease III
      Short name=Exonuclease III
      Short name=EXO III
    EC=3.1.11.2
Alternative name(s):
    AP endonuclease VI

Gene names

Name:	xthA
Ordered Locus Names:	STY1812, t1181

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase and ribonuclease H activities By similarity.
Catalytic activity	Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the DNA repair enzymes AP/exoA family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Ligand	Magnesium Metal-binding
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: InterPro exodeoxyribonuclease III activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Exodeoxyribonuclease III		PRO_0000200023
Sites
	Active site	259	1	Proton acceptor By similarity
	Metal binding	34	1	Magnesium or manganese By similarity
	Site	153	1	Important for substrate recognition By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A1B0-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 6EBBD3E6B481EEE7	FASTA	268	30,785
	10 20 30 40 50 60 MKFVSFNING LRARPHQLEA IVEKHQPDVI GLQETKVHDE MFPLEEVAKL GYNVFYHGQK 70 80 90 100 110 120 GHYGVALLTK ATPISVRRGF PDDGEEAQRR IIMAEIPSPL GNITVINGYF PQGESRDHPL 130 140 150 160 170 180 KFPAKAQFYQ NLQNYLETEL KCDNPVLIMG DMNISPTDLD IGIGEENRKR WLRTGKCSFL 190 200 210 220 230 240 PEEREWMSRL LKWGLVDTFR QANPQTMDKF SWFDYRSKGF VDNRGLRIDL LLASAPLAER 250 260 CAETGIDYDI RSMEKPSDHA PVWATFRV

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL627271 Genomic DNA. Translation: CAD02052.1. AE014613 Genomic DNA. Translation: AAO68838.1.
RefSeq	NP_456210.1. NP_804989.1.
3D structure databases
HSSP	HSSP built from PDB template 1AKO based on UniProtKB P09030.
SMR	P0A1B0. Positions 1-267.
ModBase	Search...
Proteomic databases
PRIDE	P0A1B0.
Genome annotation databases
GeneID	1070599. 1248181.
GenomeReviews	Gene locus t1181 in contig AE014613_GR. Gene locus STY1812 in contig AL513382_GR.
KEGG	stt:t1181. sty:STY1812.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A1B0.
OMA	RAMEKPS.
Enzyme and pathway databases
BioCyc	SENT209261:T1181-MON. SENT220341:STY1812-MON.
BRENDA	3.1.11.2. 3716.
Family and domain databases
InterPro	IPR000097. AP_endonuclease_F1. IPR005135. Endo/exonuclease/phosphatase. IPR004808. exoDNase_III. [Graphical view]
PANTHER	PTHR22748. ExoIII_xth. 1 hit.
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
TIGRFAMs	TIGR00195. exoDNase_III. 1 hit. TIGR00633. xth. 1 hit.
PROSITE	PS00726. AP_NUCLEASE_F1_1. 1 hit. PS00727. AP_NUCLEASE_F1_2. 1 hit. PS00728. AP_NUCLEASE_F1_3. 1 hit. PS51435. AP_NUCLEASE_F1_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EX3_SALTI

Accession

Primary (citable) accession number: P0A1B0
Secondary accession number(s): Q9Z612

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)