P0A1A9 (EX3_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exodeoxyribonuclease III
Short name=EXO III
Short name=Exonuclease III
EC=3.1.11.2

Alternative name(s):
AP endonuclease VI

Gene names

Name:	xthA
Ordered Locus Names:	STM1302

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase and ribonuclease H activities By similarity.
Catalytic activity	Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the DNA repair enzymes AP/ExoA family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Ligand	Magnesium Metal-binding
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: InterPro exodeoxyribonuclease III activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Exodeoxyribonuclease III		PRO_0000200024
Sites
	Active site	259	1	Proton acceptor By similarity
	Metal binding	34	1	Magnesium or manganese By similarity
	Site	153	1	Important for substrate recognition By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A1A9 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 6EBBD3E6B481EEE7	FASTA	268	30,785
	10 20 30 40 50 60 MKFVSFNING LRARPHQLEA IVEKHQPDVI GLQETKVHDE MFPLEEVAKL GYNVFYHGQK 70 80 90 100 110 120 GHYGVALLTK ATPISVRRGF PDDGEEAQRR IIMAEIPSPL GNITVINGYF PQGESRDHPL 130 140 150 160 170 180 KFPAKAQFYQ NLQNYLETEL KCDNPVLIMG DMNISPTDLD IGIGEENRKR WLRTGKCSFL 190 200 210 220 230 240 PEEREWMSRL LKWGLVDTFR QANPQTMDKF SWFDYRSKGF VDNRGLRIDL LLASAPLAER 250 260 CAETGIDYDI RSMEKPSDHA PVWATFRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Role of ArgR in activation of the ast operon, encoding enzymes of the arginine succinyltransferase pathway in Salmonella typhimurium." Lu C.-D., Abdelal A.T. J. Bacteriol. 181:1934-1938(1999) [PubMed: 10074092] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF108767 Genomic DNA. Translation: AAD16979.1. AE006468 Genomic DNA. Translation: AAL20227.1.
RefSeq	NP_460268.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P0A1A9.
SMR	P0A1A9. Positions 1-267.
ModBase	Search...
Proteomic databases
PRIDE	P0A1A9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1252820.
GenomeReviews	Gene locus STM1302 in contig AE006468_GR.
KEGG	stm:STM1302.
PATRIC	32381081. VBISalEnt20916_1383.
Phylogenomic databases
HOGENOM	HBG704164.
OMA	VTIMNGY.
ProtClustDB	PRK11756.
Enzyme and pathway databases
BioCyc	STYP99287:STM1302-MONOMER.
Family and domain databases
InterPro	IPR000097. AP_endonuclease_F1. IPR020847. AP_endonuclease_F1_BS. IPR020848. AP_endonuclease_F1_CS. IPR005135. Endo/exonuclease/phosphatase. IPR004808. ExoDNase_III. [Graphical view]
KO	K01142.
PANTHER	PTHR22748. ExoIII_xth. 1 hit.
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
SUPFAM	SSF56219. Exo_endo_phos. 1 hit.
TIGRFAMs	TIGR00195. ExoDNase_III. 1 hit. TIGR00633. Xth. 1 hit.
PROSITE	PS00726. AP_NUCLEASE_F1_1. 1 hit. PS00727. AP_NUCLEASE_F1_2. 1 hit. PS00728. AP_NUCLEASE_F1_3. 1 hit. PS51435. AP_NUCLEASE_F1_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EX3_SALTY

Accession

Primary (citable) accession number: P0A1A9
Secondary accession number(s): Q9Z612

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program