Reviewed, UniProtKB/Swiss-Prot P0A1A3 (ALR1_SALTY)

Names and origin

Protein names

Recommended name:
    Alanine racemase, biosynthetic
    EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	STM4247

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Subunit structure	Monomer. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.
Biophysicochemical properties	pH dependence: Optimum pH is 8.5. HAMAP MF_01201

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: HAMAP cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Alanine racemase, biosynthetic HAMAP MF_01201		PRO_0000114559
Sites
	Active site	34	1	Proton acceptor; specific for D-alanine By similarity
	Active site	255	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	34	1	N6-(pyridoxal phosphate)lysine HAMAP MF_01201
Experimental info
	Sequence conflict	5	1	T → S AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P0A1A3-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: B6527AED8263D65B	FASTA	359	39,076
	10 20 30 40 50 60 MQAATVVINR RALRHNLQRL RELAPASKLV AVVKANAYGH GLLETARTLP DADAFGVARL 70 80 90 100 110 120 EEALRLRAGG ITQPILLLEG FFDAADLPTI SAQCLHTAVH NQEQLAALEA VELAEPVTVW 130 140 150 160 170 180 MKLDTGMHRL GVRPEEAEAF YQRLTHCKNV RQPVNIVSHF ARADEPECGA TEHQLDIFNA 190 200 210 220 230 240 FCQGKPGQRS IAASGGILLW PQSHFDWARP GIILYGVSPL EHKPWGPDFG FQPVMSLTSS 250 260 270 280 290 300 LIAVRDHKAG EPVGYGGTWV SERDTRLGVV AMGYGDGYPR AAPSGTPVLV NGREVPIVGR 310 320 330 340 350 VAMDMICVDL GPNAQDNAGD PVVLWGEGLP VERIAEMTKV SAYELITRLT SRVAMKYID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biosynthetic alr alanine racemase from Salmonella typhimurium: DNA and protein sequence determination." Galakatos N.G., Daub E., Botstein D., Walsh C.T. Biochemistry 25:3255-3260(1986) [PubMed: 3524676] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[3]	"Biosynthetic alanine racemase of Salmonella typhimurium: purification and characterization of the enzyme encoded by the alr gene." Esaki N., Walsh C.T. Biochemistry 25:3261-3267(1986) [PubMed: 3524677] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-9 AND 29-46, CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M12847 Genomic DNA. Translation: AAA27022.1. AE006468 Genomic DNA. Translation: AAL23071.1.
PIR	A24102.
RefSeq	NP_463112.1.
3D structure databases
SMR	P0A1A3. Positions 1-359.
ModBase	Search...
Proteomic databases
PRIDE	P0A1A3.
Genome annotation databases
GeneID	1255773.
GenomeReviews	Gene locus STM4247 in contig AE006468_GR.
KEGG	stm:STM4247.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG712172.
OMA	EGTPVWV.
Enzyme and pathway databases
BioCyc	STYP99287:STM4247-MONOMER.
BRENDA	5.1.1.1. 2.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR1_SALTY

Accession

Primary (citable) accession number: P0A1A3
Secondary accession number(s): P06655

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)