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P0A171

- RP54_PSEPK

UniProt

P0A171 - RP54_PSEPK

Protein

RNA polymerase sigma-54 factor

Gene

rpoN

Organism
Pseudomonas putida (strain KT2440)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (01 Mar 2005)
      Previous versions | rss
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    Functioni

    Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the xylCAB operon and the xylS gene. The open complex (sigma-54 and core RNA polymerase) serves as the receptor for receipt of the melting signal from the remotely bound activator protein XylR for the expression of the xylCAB operon and xylS.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi386 – 40520H-T-H motifSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA-directed RNA polymerase activity Source: UniProtKB-KW
    3. identical protein binding Source: IntAct
    4. sequence-specific DNA binding transcription factor activity Source: InterPro
    5. sigma factor activity Source: UniProtKB-KW

    GO - Biological processi

    1. DNA-templated transcription, initiation Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Sigma factor, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciPPUT160488:GIXO-984-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA polymerase sigma-54 factor
    Gene namesi
    Name:rpoN
    Synonyms:ntrA
    Ordered Locus Names:PP_0952
    OrganismiPseudomonas putida (strain KT2440)
    Taxonomic identifieri160488 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000000556: Chromosome

    Subcellular locationi

    Keywords - Cellular componenti

    DNA-directed RNA polymerase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 497497RNA polymerase sigma-54 factorPRO_0000205535Add
    BLAST

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself6EBI-8302876,EBI-8302876

    Protein-protein interaction databases

    MINTiMINT-8407296.
    STRINGi160488.PP_0952.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A171.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi474 – 4829RPON box

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi11 – 3525Gln-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the sigma-54 factor family.Curated

    Phylogenomic databases

    eggNOGiCOG1508.
    HOGENOMiHOG000071905.
    KOiK03092.
    OMAiTQKFMHT.
    OrthoDBiEOG60CWN7.

    Family and domain databases

    InterProiIPR000394. RNA_pol_sigma_54.
    IPR007046. RNA_pol_sigma_54_core-bd.
    IPR007634. RNA_pol_sigma_54_DNA-bd.
    [Graphical view]
    PfamiPF00309. Sigma54_AID. 1 hit.
    PF04963. Sigma54_CBD. 1 hit.
    PF04552. Sigma54_DBD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000774. RpoN. 1 hit.
    PRINTSiPR00045. SIGMA54FCT.
    TIGRFAMsiTIGR02395. rpoN_sigma. 1 hit.
    PROSITEiPS00717. SIGMA54_1. 1 hit.
    PS00718. SIGMA54_2. 1 hit.
    PS50044. SIGMA54_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A171-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKPSLVLKMG QQLTMTPQLQ QAIRLLQLST LDLQQEIQEA LESNPMLERQ    50
    EDGEDFDNSD PMADNAENKP AAEVQDNSFQ ESTVSADNLE DGEWSERIPN 100
    ELPVDTAWED IYQTSASSLP SNDDDEWDFT TRTSAGESLQ SHLLWQLNLA 150
    PMSDTDRLIA VTLIDSINGQ GYLEDTLEEI CAGFDPELDI ELDEVEAVLH 200
    RIQQFEPAGV GARNLGECLL LQLRQLPATT PWMTEAKRLV TDFIDLLGSR 250
    DYSQLMRRMK IKEDELRQVI ELVQSLNPRP GSQIESSEPE YVVPDVIVRK 300
    DSDRWLVELN QEAIPRLRVN PQYAGFVRRA DTSADNTFMR NQLQEARWFI 350
    KSLQSRNETL MKVATQIVEH QRGFLDHGDE AMKPLVLHDI AEAVGMHEST 400
    ISRVTTQKYM HTPRGIYELK YFFSSHVSTS EGGECSSTAI RAIIKKLVAA 450
    ENQKKPLSDS KIAGLLEAQG IQVARRTVAK YRESLGIAPS SERKRLM 497
    Length:497
    Mass (Da):56,215
    Last modified:March 1, 2005 - v1
    Checksum:i921E788B48B5B2F8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti181 – 1811C → S in CAA34494. (PubMed:2602128)Curated
    Sequence conflicti366 – 3661Q → R in CAA34494. (PubMed:2602128)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16474 Genomic DNA. Translation: CAA34494.1.
    AE015451 Genomic DNA. Translation: AAN66577.1.
    PIRiT01753.
    RefSeqiNP_743113.1. NC_002947.3.

    Genome annotation databases

    EnsemblBacteriaiAAN66577; AAN66577; PP_0952.
    GeneIDi1044867.
    KEGGippu:PP_0952.
    PATRICi19940054. VBIPsePut30601_1013.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16474 Genomic DNA. Translation: CAA34494.1 .
    AE015451 Genomic DNA. Translation: AAN66577.1 .
    PIRi T01753.
    RefSeqi NP_743113.1. NC_002947.3.

    3D structure databases

    ProteinModelPortali P0A171.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-8407296.
    STRINGi 160488.PP_0952.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN66577 ; AAN66577 ; PP_0952 .
    GeneIDi 1044867.
    KEGGi ppu:PP_0952.
    PATRICi 19940054. VBIPsePut30601_1013.

    Phylogenomic databases

    eggNOGi COG1508.
    HOGENOMi HOG000071905.
    KOi K03092.
    OMAi TQKFMHT.
    OrthoDBi EOG60CWN7.

    Enzyme and pathway databases

    BioCyci PPUT160488:GIXO-984-MONOMER.

    Family and domain databases

    InterProi IPR000394. RNA_pol_sigma_54.
    IPR007046. RNA_pol_sigma_54_core-bd.
    IPR007634. RNA_pol_sigma_54_DNA-bd.
    [Graphical view ]
    Pfami PF00309. Sigma54_AID. 1 hit.
    PF04963. Sigma54_CBD. 1 hit.
    PF04552. Sigma54_DBD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000774. RpoN. 1 hit.
    PRINTSi PR00045. SIGMA54FCT.
    TIGRFAMsi TIGR02395. rpoN_sigma. 1 hit.
    PROSITEi PS00717. SIGMA54_1. 1 hit.
    PS00718. SIGMA54_2. 1 hit.
    PS50044. SIGMA54_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide and deduced amino acid sequence of the RpoN sigma-factor of Pseudomonas putida."
      Koehler T., Cayrol J.M., Ramos J.L., Harayama S.
      Nucleic Acids Res. 17:10125-10125(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: KT2440.

    Entry informationi

    Entry nameiRP54_PSEPK
    AccessioniPrimary (citable) accession number: P0A171
    Secondary accession number(s): P15591
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3