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P0A171

- RP54_PSEPK

UniProt

P0A171 - RP54_PSEPK

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Protein

RNA polymerase sigma-54 factor

Gene
rpoN, ntrA, PP_0952
Organism
Pseudomonas putida (strain KT2440)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the xylCAB operon and the xylS gene. The open complex (sigma-54 and core RNA polymerase) serves as the receptor for receipt of the melting signal from the remotely bound activator protein XylR for the expression of the xylCAB operon and xylS.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi386 – 40520H-T-H motif Reviewed predictionAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA-directed RNA polymerase activity Source: UniProtKB-KW
  3. identical protein binding Source: IntAct
  4. sequence-specific DNA binding transcription factor activity Source: InterPro
  5. sigma factor activity Source: UniProtKB-KW

GO - Biological processi

  1. DNA-templated transcription, initiation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Sigma factor, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciPPUT160488:GIXO-984-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma-54 factor
Gene namesi
Name:rpoN
Synonyms:ntrA
Ordered Locus Names:PP_0952
OrganismiPseudomonas putida (strain KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000556: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 497497RNA polymerase sigma-54 factorPRO_0000205535Add
BLAST

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-8302876,EBI-8302876

Protein-protein interaction databases

MINTiMINT-8407296.
STRINGi160488.PP_0952.

Structurei

3D structure databases

ProteinModelPortaliP0A171.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi474 – 4829RPON box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi11 – 3525Gln-richAdd
BLAST

Sequence similaritiesi

Belongs to the sigma-54 factor family.

Phylogenomic databases

eggNOGiCOG1508.
HOGENOMiHOG000071905.
KOiK03092.
OMAiTQKFMHT.
OrthoDBiEOG60CWN7.

Family and domain databases

InterProiIPR000394. RNA_pol_sigma_54.
IPR007046. RNA_pol_sigma_54_core-bd.
IPR007634. RNA_pol_sigma_54_DNA-bd.
[Graphical view]
PfamiPF00309. Sigma54_AID. 1 hit.
PF04963. Sigma54_CBD. 1 hit.
PF04552. Sigma54_DBD. 1 hit.
[Graphical view]
PIRSFiPIRSF000774. RpoN. 1 hit.
PRINTSiPR00045. SIGMA54FCT.
TIGRFAMsiTIGR02395. rpoN_sigma. 1 hit.
PROSITEiPS00717. SIGMA54_1. 1 hit.
PS00718. SIGMA54_2. 1 hit.
PS50044. SIGMA54_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A171-1 [UniParc]FASTAAdd to Basket

« Hide

MKPSLVLKMG QQLTMTPQLQ QAIRLLQLST LDLQQEIQEA LESNPMLERQ    50
EDGEDFDNSD PMADNAENKP AAEVQDNSFQ ESTVSADNLE DGEWSERIPN 100
ELPVDTAWED IYQTSASSLP SNDDDEWDFT TRTSAGESLQ SHLLWQLNLA 150
PMSDTDRLIA VTLIDSINGQ GYLEDTLEEI CAGFDPELDI ELDEVEAVLH 200
RIQQFEPAGV GARNLGECLL LQLRQLPATT PWMTEAKRLV TDFIDLLGSR 250
DYSQLMRRMK IKEDELRQVI ELVQSLNPRP GSQIESSEPE YVVPDVIVRK 300
DSDRWLVELN QEAIPRLRVN PQYAGFVRRA DTSADNTFMR NQLQEARWFI 350
KSLQSRNETL MKVATQIVEH QRGFLDHGDE AMKPLVLHDI AEAVGMHEST 400
ISRVTTQKYM HTPRGIYELK YFFSSHVSTS EGGECSSTAI RAIIKKLVAA 450
ENQKKPLSDS KIAGLLEAQG IQVARRTVAK YRESLGIAPS SERKRLM 497
Length:497
Mass (Da):56,215
Last modified:March 1, 2005 - v1
Checksum:i921E788B48B5B2F8
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti181 – 1811C → S in CAA34494. 1 Publication
Sequence conflicti366 – 3661Q → R in CAA34494. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16474 Genomic DNA. Translation: CAA34494.1.
AE015451 Genomic DNA. Translation: AAN66577.1.
PIRiT01753.
RefSeqiNP_743113.1. NC_002947.3.

Genome annotation databases

EnsemblBacteriaiAAN66577; AAN66577; PP_0952.
GeneIDi1044867.
KEGGippu:PP_0952.
PATRICi19940054. VBIPsePut30601_1013.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16474 Genomic DNA. Translation: CAA34494.1 .
AE015451 Genomic DNA. Translation: AAN66577.1 .
PIRi T01753.
RefSeqi NP_743113.1. NC_002947.3.

3D structure databases

ProteinModelPortali P0A171.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-8407296.
STRINGi 160488.PP_0952.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN66577 ; AAN66577 ; PP_0952 .
GeneIDi 1044867.
KEGGi ppu:PP_0952.
PATRICi 19940054. VBIPsePut30601_1013.

Phylogenomic databases

eggNOGi COG1508.
HOGENOMi HOG000071905.
KOi K03092.
OMAi TQKFMHT.
OrthoDBi EOG60CWN7.

Enzyme and pathway databases

BioCyci PPUT160488:GIXO-984-MONOMER.

Family and domain databases

InterProi IPR000394. RNA_pol_sigma_54.
IPR007046. RNA_pol_sigma_54_core-bd.
IPR007634. RNA_pol_sigma_54_DNA-bd.
[Graphical view ]
Pfami PF00309. Sigma54_AID. 1 hit.
PF04963. Sigma54_CBD. 1 hit.
PF04552. Sigma54_DBD. 1 hit.
[Graphical view ]
PIRSFi PIRSF000774. RpoN. 1 hit.
PRINTSi PR00045. SIGMA54FCT.
TIGRFAMsi TIGR02395. rpoN_sigma. 1 hit.
PROSITEi PS00717. SIGMA54_1. 1 hit.
PS00718. SIGMA54_2. 1 hit.
PS50044. SIGMA54_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide and deduced amino acid sequence of the RpoN sigma-factor of Pseudomonas putida."
    Koehler T., Cayrol J.M., Ramos J.L., Harayama S.
    Nucleic Acids Res. 17:10125-10125(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KT2440.

Entry informationi

Entry nameiRP54_PSEPK
AccessioniPrimary (citable) accession number: P0A171
Secondary accession number(s): P15591
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: May 14, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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