P0A170 (NAHB_PSEU8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase
EC=1.3.1.29

Alternative name(s):
Cis-naphthalene dihydrodiol dehydrogenase
Dibenzothiophene dihydrodiol dehydrogenase
EC=1.3.1.60

Gene names

Name:

doxE

Encoded on

Plasmid

Organism

Pseudomonas sp. (strain C18)

Taxonomic identifier

69011 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of naphthalene dihydrodiol into 1,2-dihydroxynaphthalene.
Catalytic activity	Cis-1,2-dihydronaphthalene-1,2-diol + NAD+ = naphthalene-1,2-diol + NADH. Cis-1,2-dihydroxy-1,2-dihydrodibenzothiophene + NAD+ = 1,2-dihydroxydibenzothiophene + NADH.
Pathway	Aromatic compound metabolism; naphthalene degradation.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Sequence caution	The sequence AAA16128.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase activity Inferred from electronic annotation. Source: EC dibenzothiophene dihydrodiol dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase		PRO_0000054731
Regions
	Nucleotide binding	8 – 32	25	NAD By similarity
Sites
	Active site	153	1	Proton acceptor By similarity
	Binding site	140	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A170 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: B77E517D88789380	FASTA	259	27,528
	10 20 30 40 50 60 MGNQQVVSIT GAGSGIGLEL VRSFKSAGYY VSALVRNEEQ EALLCKEFKD ALEIVVGDVR 70 80 90 100 110 120 DHATNEKLIK QTIDRFGHLD CFIANAGIWD YMLSIEEPWE KISSSFDEIF DINVKSYFSG 130 140 150 160 170 180 ISAALPELKK TNGSVVMTAS VSSHAVGGGG SCYIASKHAV LGMVKALAYE LAPEVRVNAV 190 200 210 220 230 240 SPGGTVTSLC GPASAGFDKM HMKDMPGIDD MIKGLTPLGF AAKPEDVVAP YLLLASRKQG 250 KFITGTVISI DGGMALGRK

References

[1]	"Metabolism of dibenzothiophene and naphthalene in Pseudomonas strains: complete DNA sequence of an upper naphthalene catabolic pathway." Denome S.A., Stanley D.C., Olson E.S., Young K.D. J. Bacteriol. 175:6890-6901(1993) [PubMed: 8226631] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M60405 Genomic DNA. Translation: AAA16128.1. Different initiation.
3D structure databases
ProteinModelPortal	P0A170.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAHB_PSEU8

Accession

Primary (citable) accession number: P0A170
Secondary accession number(s): O33462, Q52459

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program