ID   NAHB_PSEPU              Reviewed;         259 AA.
AC   P0A169; O33462; Q52459;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   16-JUN-2009, entry version 23.
DE   RecName: Full=1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase;
DE            EC=1.3.1.29;
DE   AltName: Full=Cis-naphthalene dihydrodiol dehydrogenase;
DE   AltName: Full=Dibenzothiophene dihydrodiol dehydrogenase;
DE            EC=1.3.1.60;
GN   Name=nahB;
OS   Pseudomonas putida.
OG   Plasmid NPL1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BS202;
RA   Bezborodnikov S.G., Boronin A.M., Tiedje J.M.;
RT   "Nucleotide sequences of genes encoding an upper pathway of
RT   naphthalene metabolism of NPL1 plasmid from Pseudomonas putida strain
RT   BS202.";
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the oxidation of naphthalene dihydrodiol into
CC       1,2-dihydroxynaphthalene (By similarity).
CC   -!- CATALYTIC ACTIVITY: Cis-1,2-dihydronaphthalene-1,2-diol + NAD(+) =
CC       naphthalene-1,2-diol + NADH.
CC   -!- CATALYTIC ACTIVITY: Cis-1,2-dihydroxy-1,2-dihydrodibenzothiophene
CC       + NAD(+) = 1,2-dihydroxydibenzothiophene + NADH.
CC   -!- PATHWAY: Aromatic compound metabolism; naphtalene degradation.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family.
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DR   EMBL; AF010471; AAB62709.1; -; Genomic_DNA.
DR   HSSP; P47227; 1BDB.
DR   BRENDA; 1.3.1.29; 403.
DR   BRENDA; 1.3.1.60; 403.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0018505; F:cis-1,2-dihydro-1,2-dihydroxynaphthalene de...; IEA:EC.
DR   GO; GO:0018513; F:dibenzothiophene dihydrodiol dehydrogenase ...; IEA:EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR002347; Glc/ribitol_DH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR19410; ADH_short_C2; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   PROSITE; PS00061; ADH_SHORT; FALSE_NEG.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; NAD; Oxidoreductase; Plasmid.
FT   CHAIN         1    259       1,2-dihydroxy-1,2-dihydronaphthalene
FT                                dehydrogenase.
FT                                /FTId=PRO_0000054730.
FT   NP_BIND       8     32       NAD (By similarity).
FT   ACT_SITE    153    153       Proton acceptor (By similarity).
FT   BINDING     140    140       Substrate (By similarity).
SQ   SEQUENCE   259 AA;  27518 MW;  187E517F5AA89152 CRC64;
     MGNQQVVSIT GAGSGIGLEL VRSFKSAGYY VSALVRNEEQ EALLCKEFKD ALEIVVGDVR
     DHATNEKLIK QTIDRFGHLD CFIANAGIWD YMLSIEEPWE KISSSFDEIF DINVKSYFSG
     ISAALPELKK TNGSVVMTAS VSSHAVGGGG SCYIASKHAV LGMVKALAYE LAPEVRVNAV
     SPGGTVTSLC GSASAGFDKM HMKDMPGIDD MIKGLTPLGF AAKPEDVVAP YLLLASRKQG
     KFITGTVISI DGGMALGRK
//