Reviewed, UniProtKB/Swiss-Prot P0A169 (NAHB_PSEPU)

Names and origin

Protein names

Recommended name:
    1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase
    EC=1.3.1.29
Alternative name(s):
    Cis-naphthalene dihydrodiol dehydrogenase
    Dibenzothiophene dihydrodiol dehydrogenase
    EC=1.3.1.60

Gene names

Name:

nahB

Encoded on

Plasmid NPL1

Organism

Pseudomonas putida

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of naphthalene dihydrodiol into 1,2-dihydroxynaphthalene By similarity.
Catalytic activity	Cis-1,2-dihydronaphthalene-1,2-diol + NAD+ = naphthalene-1,2-diol + NADH. Cis-1,2-dihydroxy-1,2-dihydrodibenzothiophene + NAD+ = 1,2-dihydroxydibenzothiophene + NADH.
Pathway	Aromatic compound metabolism; naphtalene degradation.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase activity Inferred from electronic annotation. Source: EC dibenzothiophene dihydrodiol dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase		PRO_0000054730
Regions
	Nucleotide binding	8 – 32	25	NAD By similarity
Sites
	Active site	153	1	Proton acceptor By similarity
	Binding site	140	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A169-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 187E517F5AA89152	FASTA	259	27,518
	10 20 30 40 50 60 MGNQQVVSIT GAGSGIGLEL VRSFKSAGYY VSALVRNEEQ EALLCKEFKD ALEIVVGDVR 70 80 90 100 110 120 DHATNEKLIK QTIDRFGHLD CFIANAGIWD YMLSIEEPWE KISSSFDEIF DINVKSYFSG 130 140 150 160 170 180 ISAALPELKK TNGSVVMTAS VSSHAVGGGG SCYIASKHAV LGMVKALAYE LAPEVRVNAV 190 200 210 220 230 240 SPGGTVTSLC GSASAGFDKM HMKDMPGIDD MIKGLTPLGF AAKPEDVVAP YLLLASRKQG 250 KFITGTVISI DGGMALGRK

References

[1]

"Nucleotide sequences of genes encoding an upper pathway of naphthalene metabolism of NPL1 plasmid from Pseudomonas putida strain BS202." Bezborodnikov S.G., Boronin A.M., Tiedje J.M. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BS202.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF010471 Genomic DNA. Translation: AAB62709.1.
3D structure databases
HSSP	HSSP built from PDB template 1BDB based on UniProtKB P47227.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.3.1.29. 403. 1.3.1.60. 403.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAHB_PSEPU

Accession

Primary (citable) accession number: P0A169
Secondary accession number(s): O33462, Q52459

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)