Reviewed, UniProtKB/Swiss-Prot P0A153 (LEXA1_PSEPK)

Names and origin

Protein names

Recommended name:
    LexA repressor 1
    EC=3.4.21.88

Gene names

Name:	lexA1
Synonyms:	lexA, lexA-1
Ordered Locus Names:	PP_2143

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity.
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	LexA repressor 1 HAMAP MF_00015		PRO_0000170069
Regions
	DNA binding	28 – 48	21	H-T-H motif By similarity
Sites
	Active site	123	1	For autocatalytic cleavage activity By similarity
	Active site	160	1	For autocatalytic cleavage activity By similarity
	Site	88 – 89	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A153-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: EF43C178C97D0679	FASTA	202	22,148
	10 20 30 40 50 60 MLKLTPRQAE ILAFIKRCLE DNGFPPTRAE IAQELGFKSP NAAEEHLKAL ARKGAIEMTP 70 80 90 100 110 120 GASRGIRIPG LEAKAEEAGL PIIGRVAAGA PILAEQHIEQ SCNINPAFFH PQADYLLRVH 130 140 150 160 170 180 GMSMKDVGIF DGDLLAVHTC REARNGQIVV ARIGDEVTVK RFKREGSKVW LLAENPEFAP 190 200 IEVDLKEQEL VIEGLSVGVI RR

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN67756.1.
RefSeq	NP_744292.1.
3D structure databases
HSSP	HSSP built from PDB template 1JHH based on UniProtKB P03033.
ModBase	Search...
Protein-protein interaction databases
STRING	P0A153.
Genome annotation databases
GeneID	1044998.
GenomeReviews	Gene locus PP_2143 in contig AE015451_GR.
KEGG	ppu:PP_2143.
NMPDR	fig|160488.1.peg.2122.
TIGR	PP_2143.
Phylogenomic databases
HOGENOM	P0A153.
OMA	KVIGVFR.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_2143-MON.
Family and domain databases
HAMAP	MF_00015. [Tree]
InterPro	IPR006199. LexA_DNA_bd. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA1_PSEPK

Accession

Primary (citable) accession number: P0A153
Secondary accession number(s): P37453, Q9EUU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)