Reviewed, UniProtKB/Swiss-Prot P0A108 (NAHC_PSEU8)

Names and origin

Protein names

Recommended name:
    1,2-dihydroxynaphthalene dioxygenase
    EC=1.13.11.-

Gene names

Name:

doxG

Encoded on

Plasmid

Organism

Pseudomonas sp. (strain C18)

Taxonomic identifier

69011 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the meta-cleavage of the substrate's aromatic ring.
Cofactor	Fe2+ ion.
Pathway	Aromatic compound metabolism; naphtalene degradation.
Sequence similarities	Belongs to the extradiol ring-cleavage dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	3D-structure Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ferrous iron binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 302

302

1,2-dihydroxynaphthalene dioxygenase

PRO_0000085023

Sites

Metal binding

152

1

Iron By similarity

Metal binding

215

1

Iron By similarity

Metal binding

266

1

Iron By similarity

Secondary structure

1

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302

Helix Strand Turn

Details...

Beta strand

7 – 16

10

Helix

20 – 29

10

Beta strand

34 – 36

3

Beta strand

41 – 52

12

Beta strand

54 – 60

7

Beta strand

63 – 74

12

Helix

75 – 87

13

Helix

97 – 103

7

Beta strand

105 – 112

8

Beta strand

118 – 127

10

Helix

146 – 148

3

Beta strand

152 – 155

4

Helix

160 – 169

10

Beta strand

176 – 181

6

Beta strand

187 – 199

13

Beta strand

201 – 204

4

Beta strand

210 – 222

13

Helix

223 – 235

13

Beta strand

240 – 246

7

Turn

248 – 250

3

Beta strand

253 – 258

6

Beta strand

262 – 269

8

Beta strand

280 – 283

4

Beta strand

285 – 287

3

Beta strand

294 – 296

3

Sequences

	Sequence		Length	Mass (Da)
	P0A108-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: C2A3DAECFB6FB833	FASTA	302	33,942
	10 20 30 40 50 60 MSKQAAVIEL GYMGISVKDP DAWKSFATDM LGLQVLDEGE KDRFYLRMDY WHHRIVVHHN 70 80 90 100 110 120 GQDDLEYLGW RVAGKPEFEA LGQKLIDAGY KIRICDKVEA QERMVLGLMK TEDPGGNPTE 130 140 150 160 170 180 IFWGPRIDMS NPFHPGRPLH GKFVTGDQGL GHCIVRQTDV AEAHKFYSLL GFRGDVEYRI 190 200 210 220 230 240 PLPNGMTAEL SFMHCNARDH SIAFGAMPAA KRLNHLMLEY THMEDLGYTH QQFVKNEIDI 250 260 270 280 290 300 ALQLGIHAND KALTFYGATP SGWLIEPGWR GATAIDEAEY YVGDIFGHGV EATGYGLDVK LS

References

[1]	"Metabolism of dibenzothiophene and naphthalene in Pseudomonas strains: complete DNA sequence of an upper naphthalene catabolic pathway." Denome S.A., Stanley D.C., Olson E.S., Young K.D. J. Bacteriol. 175:6890-6901(1993) [PubMed: 8226631] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBLGenBankDDBJ

M60405 Genomic DNA. Translation: AAA16130.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EHZ	X-ray	1.35	A	1-302	[»]
2EI0	X-ray	1.60	A	1-302	[»]
2EI1	X-ray	1.52	A	1-302	[»]
2EI2	X-ray	1.69	A	1-302	[»]
2EI3	X-ray	1.90	A	1-302	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR004360. Glyas_bleo-R_dOase.
IPR000486. Xdiol_dOase_1_2.
[Graphical view]

Pfam

PF00903. Glyoxalase. 1 hit.
[Graphical view]

ProDom

PD002334. Gly_diox. 2 hits.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00082. EXTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

NAHC_PSEU8

Accession

Primary (citable) accession number: P0A108
Secondary accession number(s): Q57145

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	November 25, 2008
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)