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Protein

3-oxoadipate CoA-transferase subunit B

Gene

pcaJ

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Succinyl-CoA + 3-oxoadipate = succinate + 3-oxoadipyl-CoA.

Pathway:ibeta-ketoadipate pathway

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA and succinyl-CoA from 3-oxoadipate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-oxoadipate CoA-transferase subunit A (pcaI), 3-oxoadipate CoA-transferase subunit B (pcaJ)
  2. Beta-ketoadipyl-CoA thiolase (pcaF)
This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA and succinyl-CoA from 3-oxoadipate, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei50 – 501PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3191.
RETL1328306-WGS:GSTH-6060-MONOMER.
BRENDAi2.8.3.6. 5092.
UniPathwayiUPA00157; UER00262.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoadipate CoA-transferase subunit B (EC:2.8.3.6)
Alternative name(s):
Beta-ketoadipate:succinyl-CoA transferase subunit B
Gene namesi
Name:pcaJ
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 2132123-oxoadipate CoA-transferase subunit BPRO_0000157919Add
BLAST

Proteomic databases

PRIDEiP0A102.

Interactioni

Subunit structurei

Heterodimer.

Protein-protein interaction databases

STRINGi160488.PP_3952.

Structurei

3D structure databases

ProteinModelPortaliP0A102.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR012791. 3-oxoacid_CoA-transf_B.
IPR004165. CoA_trans_fam_I.
IPR004164. CoA_transf_AS.
[Graphical view]
PANTHERiPTHR13707. PTHR13707. 1 hit.
PfamiPF01144. CoA_trans. 1 hit.
[Graphical view]
SMARTiSM00882. CoA_trans. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02428. pcaJ_scoB_fam. 1 hit.
PROSITEiPS01274. COA_TRANSF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A102-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTITKKLSRT EMAQRVAADI QEGAYVNLGI GAPTLVANYL GDKEVFLHSE
60 70 80 90 100
NGLLGMGPSP APGEEDDDLI NAGKQHVTLL TGGAFFHHAD SFSMMRGGHL
110 120 130 140 150
DIAVLGAFQV SVKGDLANWH TGAEGSIPAV GGAMDLATGA RQVFVMMDHL
160 170 180 190 200
TKTGESKLVP ECTYPLTGIA CVSRIYTDLA VLEVTPEGLK VVEICADIDF
210
DELQKLSGVP LIK
Length:213
Mass (Da):22,552
Last modified:January 23, 2007 - v2
Checksum:i5071B33173AD8B7B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88763 Genomic DNA. Translation: AAA25923.1.
PIRiB42985.
RefSeqiWP_003251131.1. NZ_BBNC01000011.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88763 Genomic DNA. Translation: AAA25923.1.
PIRiB42985.
RefSeqiWP_003251131.1. NZ_BBNC01000011.1.

3D structure databases

ProteinModelPortaliP0A102.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160488.PP_3952.

Proteomic databases

PRIDEiP0A102.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00157; UER00262.
BioCyciMetaCyc:MONOMER-3191.
RETL1328306-WGS:GSTH-6060-MONOMER.
BRENDAi2.8.3.6. 5092.

Family and domain databases

InterProiIPR012791. 3-oxoacid_CoA-transf_B.
IPR004165. CoA_trans_fam_I.
IPR004164. CoA_transf_AS.
[Graphical view]
PANTHERiPTHR13707. PTHR13707. 1 hit.
PfamiPF01144. CoA_trans. 1 hit.
[Graphical view]
SMARTiSM00882. CoA_trans. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02428. pcaJ_scoB_fam. 1 hit.
PROSITEiPS01274. COA_TRANSF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of the genes encoding beta-ketoadipate: succinyl-coenzyme A transferase in Pseudomonas putida."
    Parales R.E., Harwood C.S.
    J. Bacteriol. 174:4657-4666(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: PRS2000.
  2. "Evolutionarily homologous alpha 2 beta 2 oligomeric structures in beta-ketoadipate succinyl-CoA transferases from Acinetobacter calcoaceticus and Pseudomonas putida."
    Yeh W.-K., Ornston L.N.
    J. Biol. Chem. 256:1565-1569(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-7.

Entry informationi

Entry nameiPCAJ_PSEPU
AccessioniPrimary (citable) accession number: P0A102
Secondary accession number(s): Q01104
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 38 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.