P0A101 (PCAJ_PSEPK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoadipate CoA-transferase subunit B
EC=2.8.3.6

Alternative name(s):
Beta-ketoadipate:succinyl-CoA transferase subunit B

Gene names

Name:	pcaJ
Ordered Locus Names:	PP_3952

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group › Pseudomonas putida

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Succinyl-CoA + 3-oxoadipate = succinate + 3-oxoadipyl-CoA.
Pathway	Aromatic compound metabolism; beta-ketoadipate pathway; acetyl-CoA and succinyl-CoA from 3-oxoadipate: step 1/2.
Subunit structure	Heterodimer By similarity.
Sequence similarities	Belongs to the 3-oxoacid CoA-transferase subunit B family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	beta-ketoadipate pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	3-oxoadipate CoA-transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 213	212	3-oxoadipate CoA-transferase subunit B		PRO_0000157918
Sites
	Active site	50	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A101 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 5071B33173AD8B7B	FASTA	213	22,552
	10 20 30 40 50 60 MTITKKLSRT EMAQRVAADI QEGAYVNLGI GAPTLVANYL GDKEVFLHSE NGLLGMGPSP 70 80 90 100 110 120 APGEEDDDLI NAGKQHVTLL TGGAFFHHAD SFSMMRGGHL DIAVLGAFQV SVKGDLANWH 130 140 150 160 170 180 TGAEGSIPAV GGAMDLATGA RQVFVMMDHL TKTGESKLVP ECTYPLTGIA CVSRIYTDLA 190 200 210 VLEVTPEGLK VVEICADIDF DELQKLSGVP LIK

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT2440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015451 Genomic DNA. Translation: AAN69546.1.
RefSeq	NP_746082.1. NC_002947.3.
3D structure databases
ProteinModelPortal	P0A101.
ModBase	Search...
Protein-protein interaction databases
STRING	160488.PP_3952.
Proteomic databases
PRIDE	P0A101.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN69546; AAN69546; PP_3952.
GeneID	1046612.
KEGG	ppu:PP_3952.
PATRIC	19946530. VBIPsePut30601_4210.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2057.
HOGENOM	HOG000221245.
KO	K01032.
OMA	TWVMMDL.
ProtClustDB	CLSK909501.
Enzyme and pathway databases
BioCyc	PPUT160488:GIXO-3953-MONOMER.
UniPathway	UPA00157; UER00262.
Family and domain databases
InterPro	IPR012791. 3-oxoacid_CoA-transf_B. IPR004165. CoA_trans_fam_I. IPR004164. CoA_transf_AS. [Graphical view]
PANTHER	PTHR13707. PTHR13707. 1 hit.
Pfam	PF01144. CoA_trans. 1 hit. [Graphical view]
SMART	SM00882. CoA_trans. 1 hit. [Graphical view]
TIGRFAMs	TIGR02428. pcaJ_scoB_fam. 1 hit.
PROSITE	PS01274. COA_TRANSF_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCAJ_PSEPK

Accession

Primary (citable) accession number: P0A101
Secondary accession number(s): Q01104

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program