P0A0Z9 (ARGA_PSEPK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Amino-acid acetyltransferase
EC=2.3.1.1

Alternative name(s):
N-acetylglutamate synthase
Short name=AGS
Short name=NAGS

Gene names

Name:	argA
Ordered Locus Names:	PP_5185

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subcellular location	Cytoplasm Probable HAMAP MF_01105.
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Amino-acid acetyltransferase HAMAP MF_01105		PRO_0000186799
Regions
	Domain	286 – 432	147	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	P0A0Z9 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: BFB889B705F22450	FASTA	432	47,440
	10 20 30 40 50 60 MPDYVNWLRH ASPYINAHRD CTFVVMLPGD GVEHPNFGNI VHDLVLLHSL GVRLVLVHGS 70 80 90 100 110 120 RPQIESRLAD RGLTPHYHRG MRITDAATLD CVIDAVGALR LAIEARLSMD IAASPMQGSR 130 140 150 160 170 180 LRVASGNLVT ARPIGVLEGV DYHHTGEVRR VDRKGISRLL DERSIVLLSP LGYSPTGEIF 190 200 210 220 230 240 NLACEDVATR AAIELGADKL LLFGAEPGLL DADGRLVREL RPQQVAPHLQ RLGSDYQGEL 250 260 270 280 290 300 LDAAAEACKG GVARSHIVSY AEDGALLTEL FTRGGGGTLV SQEQFEVVRE ATIEDVGGLL 310 320 330 340 350 360 ELISPLEEQG ILVRRSREVL EREIEQFSVV EREGMIIACA ALYPIADSEA GELACLAVNP 370 380 390 400 410 420 EYRHGGRGDE LLERIESRAR QMGLSTLFVL TTRTAHWFRE RGFAPSGVER LPAARASLYN 430 YQRNSKIFEK PL

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT2440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015451 Genomic DNA. Translation: AAN70750.1.
RefSeq	NP_747286.1. NC_002947.3.
3D structure databases
ProteinModelPortal	P0A0Z9.
ModBase	Search...
Protein-protein interaction databases
STRING	P0A0Z9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1042487.
GenomeReviews	Gene locus PP_5185 in contig AE015451_GR.
KEGG	ppu:PP_5185.
NMPDR	fig|160488.1.peg.5116.
PATRIC	19949206. VBIPsePut30601_5532.
TIGR	PP_5185.
Phylogenomic databases
eggNOG	COG0548.
HOGENOM	HBG545264.
OMA	AFNLAME.
PhylomeDB	P0A0Z9.
ProtClustDB	PRK05279.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_5185-MONOMER.
Family and domain databases
HAMAP	MF_01105. N-acetyl_glu_synth. [Tree]
InterPro	IPR000182. AcTrfase_GCN5-related_dom. IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
KO	K14682.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF55729. Acyl_CoA_acyltransferase. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_PSEPK

Accession

Primary (citable) accession number: P0A0Z9
Secondary accession number(s): P32042

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program