P0A0X4 (RS12_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
30S ribosomal protein S12

Gene names

Name:	rpsL
Ordered Locus Names:	HP_1197

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	135 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	With S4 and S5 plays an important role in translational accuracy By similarity. HAMAP-Rule MF_00403_B Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit By similarity. HAMAP-Rule MF_00403_B
Subunit structure	Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex By similarity.
Sequence similarities	Belongs to the ribosomal protein S12P family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	RNA-binding rRNA-binding tRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	small ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular_function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 135	135	30S ribosomal protein S12 HAMAP-Rule MF_00403_B		PRO_0000146235
Amino acid modifications
	Modified residue	89	1	3-methylthioaspartic acid By similarity
Natural variations
	Natural variant	43	1	K → R in strain: CCUG 17874; streptomycin resistant.

Sequences

	Sequence		Length	Mass (Da)
	P0A0X4 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 909C91470EB19959	FASTA	135	15,106
	10 20 30 40 50 60 MPTINQLIRK ERKKVVKKTK SPALVECPQR RGVCTRVYTT TPKKPNSALR KVAKVRLTSK 70 80 90 100 110 120 FEVISYIPGE GHNLQEHSIV LVRGGRVKDL PGVKYHIVRG ALDTAGVNKR TVSRSKYGTK 130 KAKATDKKAT DNKKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
[2]	"Mutations affecting the colonizing-potential of Helicobacter pylori map within the cag pathogenicity island." Marchetti M., Censini S., Ghiara P., Rappuoli R., Covacci A. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (OF A STREPTOMYCIN RESISTANT VARIANT). Strain: DSM 4867 / CCUG 17874 / NCTC 11638.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD08241.1. U96106 Genomic DNA. Translation: AAD00825.1.
PIR	E64669.
RefSeq	NP_207988.1. NC_000915.1. YP_006935116.1. NC_018939.1.
3D structure databases
ProteinModelPortal	P0A0X4.
SMR	P0A0X4. Positions 1-123.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-160916.
STRING	85962.HP1197.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD08241; AAD08241; HP_1197.
GeneID	13870396. 899959.
KEGG	heo:C694_06190. hpy:HP1197.
PATRIC	20593751. VBIHelPyl33062_1253.
Phylogenomic databases
eggNOG	COG0048.
KO	K02950.
OMA	SCPERRG.
ProtClustDB	PRK05163.
Family and domain databases
Gene3D	2.40.50.140. 1 hit.
HAMAP	MF_00403_B. Ribosomal_S12_B.
InterPro	IPR012340. NA-bd_OB-fold. IPR006032. Ribosomal_S12/S23. IPR005679. Ribosomal_S12_bac. [Graphical view]
PANTHER	PTHR11652. PTHR11652. 1 hit.
Pfam	PF00164. Ribosomal_S12. 1 hit. [Graphical view]
PIRSF	PIRSF002133. Ribosomal_S12/S23. 1 hit.
PRINTS	PR01034. RIBOSOMALS12.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00981. rpsL_bact. 1 hit.
PROSITE	PS00055. RIBOSOMAL_S12. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RS12_HELPY

Accession

Primary (citable) accession number: P0A0X4
Secondary accession number(s): P56019, Q9R787

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program