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P0A0X4

- RS12_HELPY

UniProt

P0A0X4 - RS12_HELPY

Protein

30S ribosomal protein S12

Gene

rpsL

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    With S4 and S5 plays an important role in translational accuracy.By similarity
    Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. rRNA binding Source: UniProtKB-HAMAP
    3. structural constituent of ribosome Source: InterPro
    4. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. response to antibiotic Source: UniProtKB-KW
    2. translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Biological processi

    Antibiotic resistance

    Keywords - Ligandi

    RNA-binding, rRNA-binding, tRNA-binding

    Enzyme and pathway databases

    BioCyciHPY:HP1197-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    30S ribosomal protein S12
    Gene namesi
    Name:rpsL
    Ordered Locus Names:HP_1197
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000000429: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. small ribosomal subunit Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13513530S ribosomal protein S12PRO_0000146235Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei89 – 8913-methylthioaspartic acidBy similarity

    Proteomic databases

    PRIDEiP0A0X4.

    Interactioni

    Subunit structurei

    Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    rimPO256873EBI-7607319,EBI-7605525

    Protein-protein interaction databases

    IntActiP0A0X4. 3 interactions.
    MINTiMINT-160916.
    STRINGi85962.HP1197.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A0X4.
    SMRiP0A0X4. Positions 1-123.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein S12P family.Curated

    Phylogenomic databases

    eggNOGiCOG0048.
    KOiK02950.
    OMAiVARSKYG.
    OrthoDBiEOG61ZTNF.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    HAMAPiMF_00403_B. Ribosomal_S12_B.
    InterProiIPR012340. NA-bd_OB-fold.
    IPR006032. Ribosomal_S12/S23.
    IPR005679. Ribosomal_S12_bac.
    [Graphical view]
    PANTHERiPTHR11652. PTHR11652. 1 hit.
    PfamiPF00164. Ribosom_S12_S23. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002133. Ribosomal_S12/S23. 1 hit.
    PRINTSiPR01034. RIBOSOMALS12.
    SUPFAMiSSF50249. SSF50249. 1 hit.
    TIGRFAMsiTIGR00981. rpsL_bact. 1 hit.
    PROSITEiPS00055. RIBOSOMAL_S12. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A0X4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPTINQLIRK ERKKVVKKTK SPALVECPQR RGVCTRVYTT TPKKPNSALR    50
    KVAKVRLTSK FEVISYIPGE GHNLQEHSIV LVRGGRVKDL PGVKYHIVRG 100
    ALDTAGVNKR TVSRSKYGTK KAKATDKKAT DNKKK 135
    Length:135
    Mass (Da):15,106
    Last modified:March 1, 2005 - v1
    Checksum:i909C91470EB19959
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti43 – 431K → R in strain: CCUG 17874; streptomycin resistant.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD08241.1.
    U96106 Genomic DNA. Translation: AAD00825.1.
    PIRiE64669.
    RefSeqiNP_207988.1. NC_000915.1.
    YP_006935116.1. NC_018939.1.

    Genome annotation databases

    EnsemblBacteriaiAAD08241; AAD08241; HP_1197.
    GeneIDi13870396.
    899959.
    KEGGiheo:C694_06190.
    hpy:HP1197.
    PATRICi20593751. VBIHelPyl33062_1253.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD08241.1 .
    U96106 Genomic DNA. Translation: AAD00825.1 .
    PIRi E64669.
    RefSeqi NP_207988.1. NC_000915.1.
    YP_006935116.1. NC_018939.1.

    3D structure databases

    ProteinModelPortali P0A0X4.
    SMRi P0A0X4. Positions 1-123.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P0A0X4. 3 interactions.
    MINTi MINT-160916.
    STRINGi 85962.HP1197.

    Proteomic databases

    PRIDEi P0A0X4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD08241 ; AAD08241 ; HP_1197 .
    GeneIDi 13870396.
    899959.
    KEGGi heo:C694_06190.
    hpy:HP1197.
    PATRICi 20593751. VBIHelPyl33062_1253.

    Phylogenomic databases

    eggNOGi COG0048.
    KOi K02950.
    OMAi VARSKYG.
    OrthoDBi EOG61ZTNF.

    Enzyme and pathway databases

    BioCyci HPY:HP1197-MONOMER.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    HAMAPi MF_00403_B. Ribosomal_S12_B.
    InterProi IPR012340. NA-bd_OB-fold.
    IPR006032. Ribosomal_S12/S23.
    IPR005679. Ribosomal_S12_bac.
    [Graphical view ]
    PANTHERi PTHR11652. PTHR11652. 1 hit.
    Pfami PF00164. Ribosom_S12_S23. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002133. Ribosomal_S12/S23. 1 hit.
    PRINTSi PR01034. RIBOSOMALS12.
    SUPFAMi SSF50249. SSF50249. 1 hit.
    TIGRFAMsi TIGR00981. rpsL_bact. 1 hit.
    PROSITEi PS00055. RIBOSOMAL_S12. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700392 / 26695.
    2. "Mutations affecting the colonizing-potential of Helicobacter pylori map within the cag pathogenicity island."
      Marchetti M., Censini S., Ghiara P., Rappuoli R., Covacci A.
      Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (OF A STREPTOMYCIN RESISTANT VARIANT).
      Strain: DSM 4867 / CCUG 17874 / NCTC 11638.
    3. "A minimal bacterial RNase J-based degradosome is associated with translating ribosomes."
      Redko Y., Aubert S., Stachowicz A., Lenormand P., Namane A., Darfeuille F., Thibonnier M., De Reuse H.
      Nucleic Acids Res. 41:288-301(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: ATCC 700392 / 26695.

    Entry informationi

    Entry nameiRS12_HELPY
    AccessioniPrimary (citable) accession number: P0A0X4
    Secondary accession number(s): P56019, Q9R787
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3