P0A0U9 (PBP2_NEIMB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Penicillin-binding protein 2
Short name=PBP-2

Gene names

Name:	penA
Ordered Locus Names:	NMB0413

Organism

Neisseria meningitidis serogroup B (strain MC58) [Reference proteome] [HAMAP]

Taxonomic identifier

122586 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria › Neisseria meningitidis › Neisseria meningitidis serogroup B

Protein attributes

Sequence length	581 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Synthesis of cross-linked peptidoglycan from the lipid intermediates.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	Cell inner membrane; Peripheral membrane protein.
Domain	The enzyme has an N-terminal penicillin insensitive transglycosylase domain (formation of linear glycan strands) and a C-terminal penicillin-sensitive transpeptidase domain (cross-linking of the peptide subunits).
Sequence similarities	Belongs to the transpeptidase family.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	catalytic activity Inferred from electronic annotation. Source: UniProtKB-KW penicillin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 581	581	Penicillin-binding protein 2		PRO_0000195452
Sites
	Active site	310	1	Acyl-ester intermediate

Sequences

	Sequence		Length	Mass (Da)
	P0A0U9 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: BABC396F205D2F6B	FASTA	581	63,604
	10 20 30 40 50 60 MLIKSEYKPR MLPKEEQVKK PMTSNGRISF VLMAIAVLFA GLIARGLYLQ TVTYNFLKEQ 70 80 90 100 110 120 GDNRIVRTQT LPATRGTVSD RNGAVLALSA PTESLFAVPK EMKEMPSAAQ LERLSELVDV 130 140 150 160 170 180 PVDVLRNKLE QKGKSFIWIK RQLDPKVAEE VKALGLENFV FEKELKRHYP MGNLFAHVIG 190 200 210 220 230 240 FTDIDGKGQE GLELSLEDSL HGEDGAEVVL RDRQGNIVDS LDSPRNKAPK NGKDIILSLD 250 260 270 280 290 300 QRIQTLAYEE LNKAVEYHQA KAGTVVVLDA RTGEILALAN TPAYDPNRPG RADSEQRRNR 310 320 330 340 350 360 AVTDMIEPGS AIKPFVIAKA LDAGKTDLNE RLNTQPYKIG PSPVRDTHVY PSLDVRGIMQ 370 380 390 400 410 420 KSSNVGTSKL SARFGAEEMY DFYHELGIGV RMHSGFPGET AGLLRNWRRW RPIEQATMSF 430 440 450 460 470 480 GYGLQLSLLQ LARAYTALTH DGVLLPVSFE KQAVAPQGKR IFKESTAREV RNLMVSVTEP 490 500 510 520 530 540 GGTGTAGAVD GFDVGAKTGT ARKFVNGRYA DNKHIATFIG FAPAKNPRVI VAVTIDEPTA 550 560 570 580 HGYYGGVVAG PPFKKIMGGS LNILGISPTK PLTAAAVKTP S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the penicillin-binding protein 2 gene of Neisseria meningitidis." Xhang Q.-Y., Spratt B.G. Nucleic Acids Res. 17:5383-5383(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C311 / Serogroup B.
[2]	"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15276 Genomic DNA. Translation: CAA33347.1. AE002098 Genomic DNA. Translation: AAF40852.1.
PIR	S04857.
RefSeq	NP_273462.1. NC_003112.2.
3D structure databases
ProteinModelPortal	P0A0U9.
ModBase	Search...
Protein-protein interaction databases
STRING	122586.NMB0413.
Protocols and materials databases
DNASU	902529.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAF40852; AAF40852; NMB0413.
GeneID	902529.
KEGG	nme:NMB0413.
PATRIC	20356015. VBINeiMen85645_0523.
Phylogenomic databases
eggNOG	COG0768.
HOGENOM	HOG000049554.
KO	K03587.
OMA	MKEISHE.
ProtClustDB	CLSK877542.
Enzyme and pathway databases
BioCyc	NMEN122586:GHGG-414-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR005311. PBP_dimer. IPR001460. PCN-bd_Tpept. [Graphical view]
Pfam	PF03717. PBP_dimer. 1 hit. PF00905. Transpeptidase. 1 hit. [Graphical view]
SUPFAM	SSF56519. PBP_dimer. 1 hit. SSF56601. PBP_transp_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PBP2_NEIMB

Accession

Primary (citable) accession number: P0A0U9
Secondary accession number(s): P11882

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program