Reviewed, UniProtKB/Swiss-Prot P0A0T3 (LGUL_NEIMB)

Names and origin

Protein names

Recommended name:
    Lactoylglutathione lyase
    EC=4.4.1.5
Alternative name(s):
    Methylglyoxalase
    Aldoketomutase
    Glyoxalase I
      Short name=Glx I
    Ketone-aldehyde mutase
    S-D-lactoylglutathione methylglyoxal lyase

Gene names

Name:	gloA
Ordered Locus Names:	NMB0340

Organism

Neisseria meningitidis serogroup B [Complete proteome] [HAMAP]

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione By similarity.
Catalytic activity	(R)-S-lactoylglutathione = glutathione + methylglyoxal.
Cofactor	Binds 1 nickel ion per subunit By similarity.
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
Sequence similarities	Belongs to the glyoxalase I family.

Ontologies

Keywords
Ligand	Metal-binding Nickel
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	lactoylglutathione lyase activity Inferred from electronic annotation. Source: EC nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 138	138	Lactoylglutathione lyase		PRO_0000168095
Sites
	Metal binding	5	1	Nickel By similarity
	Metal binding	56	1	Nickel By similarity
	Metal binding	74	1	Nickel By similarity
	Metal binding	122	1	Nickel By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A0T3-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 3C3EFEA4FACAAFD3	FASTA	138	15,669
	10 20 30 40 50 60 MRLLHTMLRV GNLEKSLDFY QNVLGMKLLR RKDYPEGRFT LAFVGYGDET DSTVLELTHN 70 80 90 100 110 120 WDTERYDLGN AYGHIAVEVD DAYEACERVK RQGGNVVREA GPMKHGTTVI AFVEDPDGYK 130 IEFIQKKSGD DSVAYQTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Detection and characterisation of the genes encoding glyoxalase I and II from Neisseria meningitidis." Kizil G., Wilks K., Wells D., Ala'Aldeen D.A.A. J. Med. Microbiol. 49:669-673(2000) [PubMed: 10882093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SD / Serogroup B / Serotype 15 / Subtype 16.
[2]	"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y14298 Genomic DNA. Translation: CAA74673.1. AE002098 Genomic DNA. Translation: AAF40783.1.
PIR	G81211.
RefSeq	NP_273389.1.
3D structure databases
HSSP	HSSP built from PDB template 1F9Z based on UniProtKB Q59384.
ModBase	Search...
Genome annotation databases
GeneID	902455.
GenomeReviews	Gene locus NMB0340 in contig AE002098_GR.
KEGG	nme:NMB0340.
TIGR	NMB0340.
Phylogenomic databases
HOGENOM	P0A0T3.
OMA	PGPMKHG.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_0340-MON.
BRENDA	4.4.1.5. 293828.
Family and domain databases
InterPro	IPR004360. Glyas_bleo-R_dOase. IPR004361. Glyoxalase_1. IPR018146. Glyoxalase_1_CS. [Graphical view]
Pfam	PF00903. Glyoxalase. 1 hit. [Graphical view]
ProDom	PD002334. Gly_diox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00068. glyox_I. 1 hit.
PROSITE	PS00934. GLYOXALASE_I_1. 1 hit. PS00935. GLYOXALASE_I_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LGUL_NEIMB

Accession

Primary (citable) accession number: P0A0T3
Secondary accession number(s): O33393

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)