P0A0T2 (LGUL_NEIMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lactoylglutathione lyase
EC=4.4.1.5

Alternative name(s):
Aldoketomutase
Glyoxalase I
Short name=Glx I
Ketone-aldehyde mutase
Methylglyoxalase
S-D-lactoylglutathione methylglyoxal lyase

Gene names

Name:	gloA
Ordered Locus Names:	NMA2147

Organism

Neisseria meningitidis serogroup A [Complete proteome] [HAMAP]

Taxonomic identifier

65699 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione By similarity.
Catalytic activity	(R)-S-lactoylglutathione = glutathione + methylglyoxal.
Cofactor	Binds 1 nickel ion per subunit By similarity.
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
Sequence similarities	Belongs to the glyoxalase I family.

Ontologies

Keywords
Ligand	Metal-binding Nickel
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	lactoylglutathione lyase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 138	138	Lactoylglutathione lyase		PRO_0000168094
Sites
	Metal binding	5	1	Nickel By similarity
	Metal binding	56	1	Nickel By similarity
	Metal binding	74	1	Nickel By similarity
	Metal binding	122	1	Nickel By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A0T2 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 3C3EFEA4FACAAFD3	FASTA	138	15,669
	10 20 30 40 50 60 MRLLHTMLRV GNLEKSLDFY QNVLGMKLLR RKDYPEGRFT LAFVGYGDET DSTVLELTHN 70 80 90 100 110 120 WDTERYDLGN AYGHIAVEVD DAYEACERVK RQGGNVVREA GPMKHGTTVI AFVEDPDGYK 130 IEFIQKKSGD DSVAYQTA

References

[1]

"Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491." Parkhill J., Achtman M., James K.D., Bentley S.D., Churcher C.M., Klee S.R., Morelli G., Basham D., Brown D., Chillingworth T., Davies R.M., Davis P., Devlin K., Feltwell T., Hamlin N., Holroyd S., Jagels K., Leather S. , Moule S., Mungall K.L., Quail M.A., Rajandream M.A., Rutherford K.M., Simmonds M., Skelton J., Whitehead S., Spratt B.G., Barrell B.G. Nature 404:502-506(2000) [PubMed: 10761919] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Z2491 / Serogroup A / Serotype 4A.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL157959 Genomic DNA. Translation: CAM09244.1.
PIR	G81211.
RefSeq	YP_002343396.1. NC_003116.1.
3D structure databases
ProteinModelPortal	P0A0T2.
SMR	P0A0T2. Positions 1-126.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000000165; EBNEIP00000000165; EBNEIG00000000165.
GeneID	907300.
GenomeReviews	Gene locus NMA2147 in contig AL157959_GR.
KEGG	nma:NMA2147.
PATRIC	20365810. VBINeiMen132687_2594.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000021776.
HOGENOM	HBG704761.
OMA	PGPMKHG.
ProtClustDB	CLSK877504.
Enzyme and pathway databases
BioCyc	NMEN122587:NMA2147-MONOMER.
Family and domain databases
InterPro	IPR004360. Glyas_Fos-R_dOase. IPR004361. Glyoxalase_1. IPR018146. Glyoxalase_1_CS. [Graphical view]
KO	K01759.
Pfam	PF00903. Glyoxalase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00068. Glyox_I. 1 hit.
PROSITE	PS00934. GLYOXALASE_I_1. 1 hit. PS00935. GLYOXALASE_I_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LGUL_NEIMA

Accession

Primary (citable) accession number: P0A0T2
Secondary accession number(s): A1ITX5, O33393

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program