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Protein

HTH-type transcriptional regulator QacR

Gene

qacR

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor of qacA. Binds to IR1, an unusually long 28 bp operator, which is located downstream from the qacA promoter and overlaps its transcription start site. QacR is induced from its IR1 site by binding to one of many structurally dissimilar cationic lipophilic compounds, which are also substrates of QacA.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi24 – 4320H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator QacR
Gene namesi
Name:qacR
ORF Names:SAP100B_005, SAP104C_022
Encoded oniPlasmid pSK13 Publications
Plasmid SAP104C2 Publications
Plasmid pSK172 Publications
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi72 – 721C → A: Almost no loss of activity. 1 Publication
Mutagenesisi141 – 1411C → A or S: Almost no loss of activity. 1 Publication

Chemistry

DrugBankiDB01123. Proflavine.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 188188HTH-type transcriptional regulator QacRPRO_0000070609Add
BLAST

Proteomic databases

PaxDbiP0A0N4.

Interactioni

Subunit structurei

Homodimer. Binds cooperatively to DNA as a pair of dimers.

Protein-protein interaction databases

STRINGi158878.SAVP031.

Structurei

Secondary structure

1
188
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 1712Combined sources
Turni20 – 223Combined sources
Helixi25 – 328Combined sources
Helixi38 – 403Combined sources
Beta strandi42 – 454Combined sources
Helixi46 – 6621Combined sources
Helixi69 – 713Combined sources
Helixi75 – 8612Combined sources
Beta strandi90 – 923Combined sources
Helixi93 – 953Combined sources
Helixi96 – 1049Combined sources
Turni105 – 1084Combined sources
Helixi111 – 13626Combined sources
Helixi145 – 16218Combined sources
Turni163 – 1653Combined sources
Helixi168 – 18619Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JT0X-ray2.90A/B/C/D1-188[»]
1JT6X-ray2.54A/B/D/E1-188[»]
1JTXX-ray2.85A/B/D/E1-188[»]
1JTYX-ray2.97A/B/D/E1-188[»]
1JUMX-ray2.98A/B/D/E1-188[»]
1JUPX-ray2.95A/B/D/E1-188[»]
1JUSX-ray2.84A/B/D/E1-188[»]
1QVTX-ray2.89A/B/D/E1-188[»]
1QVUX-ray2.96A/B/D/E1-188[»]
1RKWX-ray2.62A/B/D/E1-188[»]
1RPWX-ray2.90A/B/C/D1-188[»]
2DTZX-ray2.80A/B/D/E1-188[»]
2GBYX-ray2.90A/B/D/E1-188[»]
2HQ5X-ray2.80A/B/D/E1-188[»]
ProteinModelPortaliP0A0N4.
SMRiP0A0N4. Positions 2-187.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A0N4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 6161HTH tetR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH tetR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG41062JF. Bacteria.
ENOG410XUF9. LUCA.
KOiK18938.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.357.10. 1 hit.
InterProiIPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
IPR013571. Tscrpt_reg_QacR_C.
[Graphical view]
PfamiPF08360. TetR_C_5. 1 hit.
PF00440. TetR_N. 1 hit.
[Graphical view]
PRINTSiPR00455. HTHTETR.
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEiPS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A0N4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLKDKILGV AKELFIKNGY NATTTGEIVK LSESSKGNLY YHFKTKENLF
60 70 80 90 100
LEILNIEESK WQEQWKKEQI KCKTNREKFY LYNELSLTTE YYYPLQNAII
110 120 130 140 150
EFYTEYYKTN SINEKMNKLE NKYIDAYHVI FKEGNLNGEW CINDVNAVSK
160 170 180
IAANAVNGIV TFTHEQNINE RIKLMNKFSQ IFLNGLSK
Length:188
Mass (Da):22,174
Last modified:March 1, 2005 - v1
Checksum:i7B91E005C8D47322
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GQ900498 Genomic DNA. Translation: ADA80841.1.
GQ900514 Genomic DNA. Translation: ADA80909.1.
GU565967 Genomic DNA. Translation: ADK23698.1.
PIRiS12393.
RefSeqiWP_001807342.1. NC_014369.1.
YP_003813122.1. NC_014369.1.

Genome annotation databases

GeneIDi9487076.
KEGGipg:9487076.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GQ900498 Genomic DNA. Translation: ADA80841.1.
GQ900514 Genomic DNA. Translation: ADA80909.1.
GU565967 Genomic DNA. Translation: ADK23698.1.
PIRiS12393.
RefSeqiWP_001807342.1. NC_014369.1.
YP_003813122.1. NC_014369.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JT0X-ray2.90A/B/C/D1-188[»]
1JT6X-ray2.54A/B/D/E1-188[»]
1JTXX-ray2.85A/B/D/E1-188[»]
1JTYX-ray2.97A/B/D/E1-188[»]
1JUMX-ray2.98A/B/D/E1-188[»]
1JUPX-ray2.95A/B/D/E1-188[»]
1JUSX-ray2.84A/B/D/E1-188[»]
1QVTX-ray2.89A/B/D/E1-188[»]
1QVUX-ray2.96A/B/D/E1-188[»]
1RKWX-ray2.62A/B/D/E1-188[»]
1RPWX-ray2.90A/B/C/D1-188[»]
2DTZX-ray2.80A/B/D/E1-188[»]
2GBYX-ray2.90A/B/D/E1-188[»]
2HQ5X-ray2.80A/B/D/E1-188[»]
ProteinModelPortaliP0A0N4.
SMRiP0A0N4. Positions 2-187.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi158878.SAVP031.

Chemistry

DrugBankiDB01123. Proflavine.

Proteomic databases

PaxDbiP0A0N4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi9487076.
KEGGipg:9487076.

Phylogenomic databases

eggNOGiENOG41062JF. Bacteria.
ENOG410XUF9. LUCA.
KOiK18938.

Miscellaneous databases

EvolutionaryTraceiP0A0N4.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.357.10. 1 hit.
InterProiIPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
IPR013571. Tscrpt_reg_QacR_C.
[Graphical view]
PfamiPF08360. TetR_C_5. 1 hit.
PF00440. TetR_N. 1 hit.
[Graphical view]
PRINTSiPR00455. HTHTETR.
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEiPS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Efflux-mediated antiseptic resistance gene qacA from Staphylococcus aureus: common ancestry with tetracycline- and sugar-transport proteins."
    Rouch D.A., Cram D.S., Diberardino D., Littlejohn T.G., Skurray R.A.
    Mol. Microbiol. 4:2051-2062(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SK18.
    Plasmid: pSK1
  2. Gill J., Borman J., Shetty J., Hostetler J., Durkin S., Montgomery B.
    Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SK6575 and SK707.
    Plasmid: pSK17 SAP104C
  3. Summers A.O., Shearer J., Wireman J.
    Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SK6575 and SK707.
    Plasmid: pSK17 SAP104C
  4. "Analysis of the prototypical Staphylococcus aureus multiresistance plasmid pSK1."
    Jensen S.O., Apisiridej S., Kwong S.M., Yang Y.H., Skurray R.A., Firth N.
    Plasmid 64:135-142(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SK18.
    Plasmid: pSK1
  5. "QacR is a repressor protein that regulates expression of the Staphylococcus aureus multidrug efflux pump QacA."
    Grkovic S., Brown M.H., Roberts N.J., Paulsen I.T., Skurray R.A.
    J. Biol. Chem. 273:18665-18673(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF N-TERMINUS.
    Strain: SK982.
    Plasmid: pSK1
  6. "The staphylococcal QacR multidrug regulator binds a correctly spaced operator as a pair of dimers."
    Grkovic S., Brown M.H., Schumacher M.A., Brennan R.G., Skurray R.A.
    J. Bacteriol. 183:7102-7109(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF CYS-72 AND CYS-141.
  7. "Structural mechanisms of QacR induction and multidrug recognition."
    Schumacher M.A., Miller M.C., Grkovic S., Brown M.H., Skurray R.A., Brennan R.G.
    Science 294:2158-2163(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.54 ANGSTROMS).
  8. "Structural basis for cooperative DNA binding by two dimers of the multidrug-binding protein QacR."
    Schumacher M.A., Miller M.C., Grkovic S., Brown M.H., Skurray R.A., Brennan R.G.
    EMBO J. 21:1210-1218(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
  9. "Deciphering the molecular basis of multidrug recognition: crystal structures of the Staphylococcus aureus multidrug binding transcription regulator QacR."
    Schumacher M.A., Brennan R.G.
    Res. Microbiol. 154:69-77(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiQACR_STAAU
AccessioniPrimary (citable) accession number: P0A0N4
Secondary accession number(s): D2JG66, P23217
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: March 16, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.