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Protein

Thymidylate synthase

Gene

thyA

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei26dUMPUniRule annotation1
Active sitei201NucleophileUniRule annotation1
Binding sitei2245,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei232dUMPUniRule annotation1
Binding sitei3175,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi181 – 182dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi221 – 224dUMPUniRule annotation4
Nucleotide bindingi262 – 264dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Synonyms:thyE, thyF
Encoded oniPlasmid pSK11 Publication
Plasmid pABU11 Publication
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001410221 – 318Thymidylate synthaseAdd BLAST318

Proteomic databases

PRIDEiP0A0M5

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP0A0M5
SMRiP0A0M5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

P0A0M5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNPFDEAYH GLCEEILEIG NRRDDRTHTG TISKFGHQLR FDLTKGFPLL
60 70 80 90 100
TTKKVSFKLV ATELLWFIKG DTNIQYLLKY NNNIWNEWAF ENYVQSDDYH
110 120 130 140 150
GPDMTDFGHR SQQDPEFNEQ YKEEMKKFKE RILNDDAFAK KYGNLGNVYG
160 170 180 190 200
KQWRDWEDKN GNHYDQLKSV IQQIKTNPNS RRHIVSAWNP TEIDSMALPP
210 220 230 240 250
CHTMFQFYVQ EGKLNCQLYQ RSADIFLGVP FNIASYALLT HLVAKECGLE
260 270 280 290 300
VGEFIHTFGD AHIYSNHMDA IHTQLSRDSY LPPQLKINTD KSIFDINYED
310
LELINYESHP AIKAPIAV
Length:318
Mass (Da):37,178
Last modified:February 15, 2005 - v1
Checksum:i4CE480445CF9653A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13290 Genomic DNA Translation: CAA31648.1
Y07536 Genomic DNA Translation: CAA68823.1
PIRiS04163 YXSAT3
RefSeqiNP_877984.1, NC_005054.1
WP_000282655.1, NZ_PJLT01000015.1
YP_002790944.1, NC_012547.1
YP_003813114.1, NC_014369.1
YP_006937651.1, NC_013320.1
YP_006938353.1, NC_013338.1

Genome annotation databases

GeneIDi13874801
13875494
2598377
7751509
9487079

Similar proteinsi

Entry informationi

Entry nameiTYSY_STAAU
AccessioniPrimary (citable) accession number: P0A0M5
Secondary accession number(s): P13954, Q59907
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: May 23, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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