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Protein

Thymidylate synthase

Gene

thyA

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei26dUMPUniRule annotation1
Active sitei201NucleophileUniRule annotation1
Binding sitei2245,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei232dUMPUniRule annotation1
Binding sitei3175,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi181 – 182dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi221 – 224dUMPUniRule annotation4
Nucleotide bindingi262 – 264dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Synonyms:thyE, thyF
Encoded oniPlasmid pSK11 Publication
Plasmid pABU11 Publication
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001410221 – 318Thymidylate synthaseAdd BLAST318

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP0A0M5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 2 hits.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A0M5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNPFDEAYH GLCEEILEIG NRRDDRTHTG TISKFGHQLR FDLTKGFPLL
60 70 80 90 100
TTKKVSFKLV ATELLWFIKG DTNIQYLLKY NNNIWNEWAF ENYVQSDDYH
110 120 130 140 150
GPDMTDFGHR SQQDPEFNEQ YKEEMKKFKE RILNDDAFAK KYGNLGNVYG
160 170 180 190 200
KQWRDWEDKN GNHYDQLKSV IQQIKTNPNS RRHIVSAWNP TEIDSMALPP
210 220 230 240 250
CHTMFQFYVQ EGKLNCQLYQ RSADIFLGVP FNIASYALLT HLVAKECGLE
260 270 280 290 300
VGEFIHTFGD AHIYSNHMDA IHTQLSRDSY LPPQLKINTD KSIFDINYED
310
LELINYESHP AIKAPIAV
Length:318
Mass (Da):37,178
Last modified:February 15, 2005 - v1
Checksum:i4CE480445CF9653A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13290 Genomic DNA. Translation: CAA31648.1.
Y07536 Genomic DNA. Translation: CAA68823.1.
PIRiS04163. YXSAT3.
RefSeqiNP_877984.1. NC_005054.1.
WP_000282655.1. NZ_LWBY01000050.1.
YP_002790944.1. NC_012547.1.
YP_003813114.1. NC_014369.1.
YP_006937651.1. NC_013320.1.
YP_006938353.1. NC_013338.1.

Genome annotation databases

GeneIDi13874801.
13875494.
2598377.
7751509.
9487079.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13290 Genomic DNA. Translation: CAA31648.1.
Y07536 Genomic DNA. Translation: CAA68823.1.
PIRiS04163. YXSAT3.
RefSeqiNP_877984.1. NC_005054.1.
WP_000282655.1. NZ_LWBY01000050.1.
YP_002790944.1. NC_012547.1.
YP_003813114.1. NC_014369.1.
YP_006937651.1. NC_013320.1.
YP_006938353.1. NC_013338.1.

3D structure databases

ProteinModelPortaliP0A0M5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13874801.
13875494.
2598377.
7751509.
9487079.

Enzyme and pathway databases

UniPathwayiUPA00575.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 2 hits.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYSY_STAAU
AccessioniPrimary (citable) accession number: P0A0M5
Secondary accession number(s): P13954, Q59907
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: November 30, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.