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P0A0M5 (TYSY_STAAU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Synonyms:thyE, thyF
Encoded onPlasmid pSK1 Ref.1
Plasmid pABU1 Ref.2
OrganismStaphylococcus aureus
Taxonomic identifier1280 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 318318Thymidylate synthase HAMAP-Rule MF_00008
PRO_0000141022

Sites

Active site2011 By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A0M5 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 4CE480445CF9653A

FASTA31837,178
        10         20         30         40         50         60 
MYNPFDEAYH GLCEEILEIG NRRDDRTHTG TISKFGHQLR FDLTKGFPLL TTKKVSFKLV 

        70         80         90        100        110        120 
ATELLWFIKG DTNIQYLLKY NNNIWNEWAF ENYVQSDDYH GPDMTDFGHR SQQDPEFNEQ 

       130        140        150        160        170        180 
YKEEMKKFKE RILNDDAFAK KYGNLGNVYG KQWRDWEDKN GNHYDQLKSV IQQIKTNPNS 

       190        200        210        220        230        240 
RRHIVSAWNP TEIDSMALPP CHTMFQFYVQ EGKLNCQLYQ RSADIFLGVP FNIASYALLT 

       250        260        270        280        290        300 
HLVAKECGLE VGEFIHTFGD AHIYSNHMDA IHTQLSRDSY LPPQLKINTD KSIFDINYED 

       310 
LELINYESHP AIKAPIAV 

« Hide

References

[1]"Trimethoprim resistance transposon Tn4003 from Staphylococcus aureus encodes genes for a dihydrofolate reductase and thymidylate synthetase flanked by three copies of IS257."
Rouch D.A., Messeroti L.J., Loo L.S.L., Jackson C.A., Skurray R.A.
Mol. Microbiol. 3:161-175(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Transposon: Tn4003.
[2]"Identical genes for trimethoprim-resistant dihydrofolate reductase from Staphylococcus aureus in Australia and central Europe."
Burdeska A., Ott M., Bannwarth W., Then R.L.
FEBS Lett. 266:159-162(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 157/4696.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X13290 Genomic DNA. Translation: CAA31648.1.
Y07536 Genomic DNA. Translation: CAA68823.1.
PIRYXSAT3. S04163.
RefSeqNP_877984.1. NC_005054.1.
YP_002790944.1. NC_012547.1.
YP_003813114.1. NC_014369.1.
YP_006937651.1. NC_013320.1.
YP_006938353.1. NC_013338.1.

3D structure databases

ProteinModelPortalP0A0M5.
SMRP0A0M5. Positions 5-318.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP0A0M5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID13874801.
13875494.
2598377.
7751509.
9487079.

Enzyme and pathway databases

UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 2 hits.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_STAAU
AccessionPrimary (citable) accession number: P0A0M5
Secondary accession number(s): P13954, Q59907
Entry history
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways