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P0A0M5

- TYSY_STAAU

UniProt

P0A0M5 - TYSY_STAAU

Protein

Thymidylate synthase

Gene

thyA

Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (15 Feb 2005)
      Previous versions | rss
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    • Comment

    Functioni

    Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei201 – 2011UniRule annotation

    GO - Molecular functioni

    1. thymidylate synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. dTMP biosynthetic process Source: UniProtKB-HAMAP
    2. dTTP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00575.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
    Short name:
    TSUniRule annotation
    Short name:
    TSaseUniRule annotation
    Gene namesi
    Name:thyAUniRule annotation
    Synonyms:thyE, thyF
    Encoded oniPlasmid pSK11 Publication
    Plasmid pABU11 Publication
    OrganismiStaphylococcus aureus
    Taxonomic identifieri1280 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 318318Thymidylate synthasePRO_0000141022Add
    BLAST

    Proteomic databases

    PRIDEiP0A0M5.

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A0M5.
    SMRiP0A0M5. Positions 5-318.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

    Family and domain databases

    Gene3Di3.30.572.10. 2 hits.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF55831. SSF55831. 1 hit.
    TIGRFAMsiTIGR03284. thym_sym. 1 hit.
    PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A0M5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYNPFDEAYH GLCEEILEIG NRRDDRTHTG TISKFGHQLR FDLTKGFPLL    50
    TTKKVSFKLV ATELLWFIKG DTNIQYLLKY NNNIWNEWAF ENYVQSDDYH 100
    GPDMTDFGHR SQQDPEFNEQ YKEEMKKFKE RILNDDAFAK KYGNLGNVYG 150
    KQWRDWEDKN GNHYDQLKSV IQQIKTNPNS RRHIVSAWNP TEIDSMALPP 200
    CHTMFQFYVQ EGKLNCQLYQ RSADIFLGVP FNIASYALLT HLVAKECGLE 250
    VGEFIHTFGD AHIYSNHMDA IHTQLSRDSY LPPQLKINTD KSIFDINYED 300
    LELINYESHP AIKAPIAV 318
    Length:318
    Mass (Da):37,178
    Last modified:February 15, 2005 - v1
    Checksum:i4CE480445CF9653A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13290 Genomic DNA. Translation: CAA31648.1.
    Y07536 Genomic DNA. Translation: CAA68823.1.
    PIRiS04163. YXSAT3.
    RefSeqiNP_877984.1. NC_005054.1.
    YP_002790944.1. NC_012547.1.
    YP_003813114.1. NC_014369.1.
    YP_006937651.1. NC_013320.1.
    YP_006938353.1. NC_013338.1.

    Genome annotation databases

    GeneIDi13874801.
    13875494.
    2598377.
    7751509.
    9487079.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13290 Genomic DNA. Translation: CAA31648.1 .
    Y07536 Genomic DNA. Translation: CAA68823.1 .
    PIRi S04163. YXSAT3.
    RefSeqi NP_877984.1. NC_005054.1.
    YP_002790944.1. NC_012547.1.
    YP_003813114.1. NC_014369.1.
    YP_006937651.1. NC_013320.1.
    YP_006938353.1. NC_013338.1.

    3D structure databases

    ProteinModelPortali P0A0M5.
    SMRi P0A0M5. Positions 5-318.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P0A0M5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 13874801.
    13875494.
    2598377.
    7751509.
    9487079.

    Enzyme and pathway databases

    UniPathwayi UPA00575 .

    Family and domain databases

    Gene3Di 3.30.572.10. 2 hits.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF55831. SSF55831. 1 hit.
    TIGRFAMsi TIGR03284. thym_sym. 1 hit.
    PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Trimethoprim resistance transposon Tn4003 from Staphylococcus aureus encodes genes for a dihydrofolate reductase and thymidylate synthetase flanked by three copies of IS257."
      Rouch D.A., Messeroti L.J., Loo L.S.L., Jackson C.A., Skurray R.A.
      Mol. Microbiol. 3:161-175(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Transposon: Tn4003.
      Plasmid: pSK1
    2. "Identical genes for trimethoprim-resistant dihydrofolate reductase from Staphylococcus aureus in Australia and central Europe."
      Burdeska A., Ott M., Bannwarth W., Then R.L.
      FEBS Lett. 266:159-162(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 157/4696.
      Plasmid: pABU1

    Entry informationi

    Entry nameiTYSY_STAAU
    AccessioniPrimary (citable) accession number: P0A0M5
    Secondary accession number(s): P13954, Q59907
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 15, 2005
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3