P0A0M5 (TYSY_STAAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Synonyms:	thyE, thyF

Encoded on

Plasmid pSK1 Ref.1
Plasmid pABU1 Ref.2

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008
Subunit structure	Homodimer.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Thymidylate synthase HAMAP-Rule MF_00008		PRO_0000141022
Sites
	Active site	201	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A0M5 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 4CE480445CF9653A	FASTA	318	37,178
	10 20 30 40 50 60 MYNPFDEAYH GLCEEILEIG NRRDDRTHTG TISKFGHQLR FDLTKGFPLL TTKKVSFKLV 70 80 90 100 110 120 ATELLWFIKG DTNIQYLLKY NNNIWNEWAF ENYVQSDDYH GPDMTDFGHR SQQDPEFNEQ 130 140 150 160 170 180 YKEEMKKFKE RILNDDAFAK KYGNLGNVYG KQWRDWEDKN GNHYDQLKSV IQQIKTNPNS 190 200 210 220 230 240 RRHIVSAWNP TEIDSMALPP CHTMFQFYVQ EGKLNCQLYQ RSADIFLGVP FNIASYALLT 250 260 270 280 290 300 HLVAKECGLE VGEFIHTFGD AHIYSNHMDA IHTQLSRDSY LPPQLKINTD KSIFDINYED 310 LELINYESHP AIKAPIAV

References

[1]	"Trimethoprim resistance transposon Tn4003 from Staphylococcus aureus encodes genes for a dihydrofolate reductase and thymidylate synthetase flanked by three copies of IS257." Rouch D.A., Messeroti L.J., Loo L.S.L., Jackson C.A., Skurray R.A. Mol. Microbiol. 3:161-175(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Transposon: Tn4003.
[2]	"Identical genes for trimethoprim-resistant dihydrofolate reductase from Staphylococcus aureus in Australia and central Europe." Burdeska A., Ott M., Bannwarth W., Then R.L. FEBS Lett. 266:159-162(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 157/4696.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13290 Genomic DNA. Translation: CAA31648.1. Y07536 Genomic DNA. Translation: CAA68823.1.
PIR	YXSAT3. S04163.
RefSeq	NP_877984.1. NC_005054.1. YP_002790944.1. NC_012547.1. YP_003813114.1. NC_014369.1. YP_006937651.1. NC_013320.1. YP_006938353.1. NC_013338.1.
3D structure databases
ProteinModelPortal	P0A0M5.
SMR	P0A0M5. Positions 5-318.
ModBase	Search...
Proteomic databases
PRIDE	P0A0M5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	13874801. 13875494. 2598377. 7751509. 9487079.
Phylogenomic databases
ProtClustDB	PRK01827.
Enzyme and pathway databases
UniPathway	UPA00575.
Family and domain databases
Gene3D	3.30.572.10. 2 hits.
HAMAP	MF_00008. Thymidy_synth_bact.
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_STAAU

Accession

Primary (citable) accession number: P0A0M5
Secondary accession number(s): P13954, Q59907

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	February 15, 2005
Last modified:	April 3, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program