P0A0M0 (ETXH_STAAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enterotoxin type H

Alternative name(s):
SEH

Gene names

Name:	entH
Synonyms:	seh

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Staphylococcal enterotoxins cause the intoxication staphylococcal food poisoning syndrome. The illness characterized by high fever, hypotension, diarrhea, shock, and in some cases death.
Cofactor	Binds 1 zinc ion per subunit. The zinc ion is necessary for the toxin interaction with MHC class II.
Subcellular location	Secreted.
Sequence similarities	Belongs to the staphylococcal/streptococcal toxin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Enterotoxin Superantigen Toxin
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Chain

25 – 241

217

Enterotoxin type H

PRO_0000035617

Sites

Metal binding

230

1

Zinc

Metal binding

232

1

Zinc

Amino acid modifications

Disulfide bond

106 ↔ 116

Secondary structure

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241

Helix Strand Turn

Details...

Helix

30 – 32

3

Helix

35 – 46

12

Beta strand

49 – 56

8

Turn

61 – 63

3

Beta strand

64 – 67

4

Helix

72 – 74

3

Beta strand

76 – 80

5

Helix

84 – 90

7

Beta strand

93 – 99

7

Beta strand

110 – 112

3

Beta strand

114 – 119

6

Beta strand

121 – 123

3

Beta strand

127 – 140

14

Beta strand

148 – 158

11

Helix

159 – 174

16

Beta strand

178 – 182

5

Beta strand

185 – 195

11

Beta strand

197 – 201

5

Beta strand

206 – 209

4

Helix

210 – 213

4

Helix

214 – 217

4

Beta strand

222 – 224

3

Helix

225 – 227

3

Beta strand

228 – 236

9

Sequences

	Sequence		Length	Mass (Da)
	P0A0M0 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 70F77985877616CE	FASTA	241	27,859
	10 20 30 40 50 60 MINKIKILFS FLALLLSFTS YAKAEDLHDK SELTDLALAN AYGQYNHPFI KENIKSDEIS 70 80 90 100 110 120 GEKDLIFRNQ GDSGNDLRVK FATADLAQKF KNKNVDIYGA SFYYKCEKIS ENISECLYGG 130 140 150 160 170 180 TTLNSEKLAQ ERVIGANVWV DGIQKETELI RTNKKNVTLQ ELDIKIRKIL SDKYKIYYKD 190 200 210 220 230 240 SEISKGLIEF DMKTPRDYSF DIYDLKGEND YEIDKIYEDN KTLKSDDISH IDVNLYTKKK V

References

[1]	"Characterization and biological properties of a new staphylococcal exotoxin." Ren K., Bannan J.D., Pancholi V., Cheung A.L., Robbins J.C., Fischetti V.A., Zabriskie J.B. J. Exp. Med. 180:1675-1683(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION. Strain: D4508.
[2]	"The crystal structure of staphylococcal enterotoxin H: implications for binding properties to MHC class II and TcR molecules." Haekansson M., Petersson K., Nilsson H., Forsberg G., Bjoerk P., Antonsson P., Svensson L.A. J. Mol. Biol. 302:527-537(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.69 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U11702 Genomic DNA. Translation: AAA19777.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ENF	X-ray	1.69	A	26-237	[»]
1EWC	X-ray	1.95	A	26-239	[»]
1F77	X-ray	2.40	A/B	25-241	[»]
1HXY	X-ray	2.60	D	25-237	[»]
2XN9	X-ray	2.30	C	25-241	[»]
2XNA	X-ray	2.10	C	25-241	[»]

ProteinModelPortal

P0A0M0.

SMR

P0A0M0. Positions 26-237.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.10.20.120. 1 hit.

InterPro

IPR008992. Enterotoxin_bac.
IPR006126. Staph/Strept_toxin_CS.
IPR006173. Staph_tox_OB.
IPR016091. SuperAg_toxin_C.
IPR013307. Superantigen_bac.
IPR006123. Toxin_b-grasp_Staph/Strep.
IPR006177. Toxin_bac.
[Graphical view]

Pfam

PF02876. Stap_Strp_tox_C. 1 hit.
PF01123. Stap_Strp_toxin. 1 hit.
[Graphical view]

PRINTS

PR00279. BACTRLTOXIN.
PR01898. SAGSUPRFAMLY.

SUPFAM

SSF50203. Bact_endotox. 1 hit.
SSF54334. Stap/Strep_toxin. 1 hit.

PROSITE

PS00277. STAPH_STREP_TOXIN_1. False negative.
PS00278. STAPH_STREP_TOXIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0A0M0.

Entry information

Entry name

ETXH_STAAU

Accession

Primary (citable) accession number: P0A0M0
Secondary accession number(s): Q53585

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	February 15, 2005
Last modified:	May 1, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program