P0A0J3 (SODM1_STAA8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Superoxide dismutase [Mn] 1
EC=1.15.1.1

Gene names

Name:	sodA
Ordered Locus Names:	SAOUHSC_01653

Organism

Staphylococcus aureus (strain NCTC 8325) [Reference proteome] [HAMAP]

Taxonomic identifier

93061 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. May play a role in maintaining cell viability throughout all stages of growth, but may be the major SOD activity in the exponential growth-phase. Has a role in resisting external superoxide stress. Involved in acid tolerance and the acid-adaptive response. Mediates the derepression of perR regulon in the response to HOCl stress when the level of SOD activity is low. Ref.1 Ref.5 Ref.6
Catalytic activity	2 superoxide + 2 H+ = O2 + H2O2. Ref.1 Ref.3 Ref.4
Cofactor	Binds 1 manganese ion per subunit By similarity. Ref.1
Subunit structure	Homodimer. Can also form a heterodimer with SodM. Ref.4
Induction	Transcriptionally induced by internally generated superoxide stress in a manganese-dependent way. The presence of manganese increases SodA homodimer activity and simultaneously decreases SodM homodimer activity. This occurs primarily due to post-transcriptional effects, since the expression of the gene is independent of manganese availability in the absence of superoxide generating compounds. Ref.5
Miscellaneous	According to Ref.1 the levels of SodA activity and sodA expression are growth-phase dependent, occurring most during post-exponential phase. This response was also dependent on the level of aeration, with highest activity and expression occurring under high aeration. Transcribed from two sigma-A-type promoters (PA1 and PA2). Transcriptional data show an indirect repression of PA1 promoter by sigma-B.
Sequence similarities	Belongs to the iron/manganese superoxide dismutase family.

Ontologies

Keywords
Biological process	Stress response
Ligand	Manganese Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to stress Inferred from electronic annotation. Source: UniProtKB-KW superoxide metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW superoxide dismutase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 199	199	Superoxide dismutase [Mn] 1		PRO_0000160075
Sites
	Metal binding	27	1	Manganese By similarity
	Metal binding	81	1	Manganese By similarity
	Metal binding	161	1	Manganese By similarity
	Metal binding	165	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A0J3 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: F2CEC7B4701AC559	FASTA	199	22,711
	10 20 30 40 50 60 MAFELPKLPY AFDALEPHFD KETMEIHHDR HHNTYVTKLN AAVEGTDLES KSIEEIVANL 70 80 90 100 110 120 DSVPANIQTA VRNNGGGHLN HSLFWELLSP NSEEKGTVVE KIKEQWGSLE EFKKEFADKA 130 140 150 160 170 180 AARFGSGWAW LVVNNGQLEI VTTPNQDNPL TEGKTPILGL DVWEHAYYLK YQNKRPDYIG 190 AFWNVVNWEK VDELYNATK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the major superoxide dismutase of Staphylococcus aureus and its role in starvation survival, stress resistance, and pathogenicity." Clements M.O., Watson S.P., Foster S.J. J. Bacteriol. 181:3898-3903(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], EXPRESSION, ENZYMATIC ACTIVITY, COFACTOR, FUNCTION.
[2]	"The Staphylococcus aureus NCTC8325 genome." Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 8325.
[3]	"Identification and characterization of a second superoxide dismutase gene (sodM) from Staphylococcus aureus." Wright Valderas M., Hart M.E. J. Bacteriol. 183:3399-3407(2001) [PubMed] [Europe PMC] [Abstract] Cited for: ENZYMATIC ACTIVITY.
[4]	"The superoxide dismutase gene sodM is unique to Staphylococcus aureus: absence of sodM in coagulase-negative staphylococci." Wright Valderas M., Gatson J.W., Wreyford N., Hart M.E. J. Bacteriol. 184:2465-2472(2002) [PubMed] [Europe PMC] [Abstract] Cited for: ENZYMATIC ACTIVITY, SUBUNIT.
[5]	"Role and regulation of the superoxide dismutases of Staphylococcus aureus." Karavolos M.H., Horsburgh M.J., Ingham E., Foster S.J. Microbiology 149:2749-2758(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN OXIDATIVE STRESS RESISTANCE, EXPRESSION, REGULATION.
[6]	"The impairment of superoxide dismutase coordinates the derepression of the perR regulon in the response of Staphylococcus aureus to HOCl stress." Maalej S., Dammak I., Dukan S. Microbiology 152:855-861(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN REGULATION OF PERR REGULON.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF121672 Genomic DNA. Translation: AAD17309.1. CP000253 Genomic DNA. Translation: ABD30729.1.
RefSeq	YP_500165.1. NC_007795.1.
3D structure databases
ProteinModelPortal	P0A0J3.
SMR	P0A0J3. Positions 2-196.
ModBase	Search...
Protein-protein interaction databases
STRING	93061.SAOUHSC_01653.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABD30729; ABD30729; SAOUHSC_01653.
GeneID	3920105.
KEGG	sao:SAOUHSC_01653.
PATRIC	19580689. VBIStaAur99865_1504.
Phylogenomic databases
eggNOG	COG0605.
HOGENOM	HOG000013583.
KO	K04564.
OMA	PPKHELL.
ProtClustDB	CLSK884424.
Enzyme and pathway databases
BioCyc	SAUR93061:GIWJ-1579-MONOMER.
Family and domain databases
InterPro	IPR001189. Mn/Fe_SOD. IPR019833. Mn/Fe_SOD_BS. IPR019832. Mn/Fe_SOD_C. IPR019831. Mn/Fe_SOD_N. [Graphical view]
PANTHER	PTHR11404. PTHR11404. 1 hit.
Pfam	PF02777. Sod_Fe_C. 1 hit. PF00081. Sod_Fe_N. 1 hit. [Graphical view]
PIRSF	PIRSF000349. SODismutase. 1 hit.
PRINTS	PR01703. MNSODISMTASE.
SUPFAM	SSF46609. SODismutase. 1 hit. SSF54719. SODismutase. 1 hit.
PROSITE	PS00088. SOD_MN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SODM1_STAA8

Accession

Primary (citable) accession number: P0A0J3
Secondary accession number(s): Q2FY20, Q9Z5W5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program