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P0A0J0

- SIGA_STAA8

UniProt

P0A0J0 - SIGA_STAA8

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Protein

RNA polymerase sigma factor SigA

Gene

sigA

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.1 PublicationUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi329 – 34820H-T-H motifUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-HAMAP
  2. sequence-specific DNA binding transcription factor activity Source: InterPro
  3. sigma factor activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. transcription initiation from bacterial-type RNA polymerase promoter Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Sigma factor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciSAUR93061:GIWJ-1587-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma factor SigAUniRule annotation
Gene namesi
Name:sigAUniRule annotation
Synonyms:plaC, rpoD
Ordered Locus Names:SAOUHSC_01662
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000008816: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368RNA polymerase sigma factor SigAPRO_0000093916Add
BLAST

Proteomic databases

PRIDEiP0A0J0.

Interactioni

Subunit structurei

Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription (By similarity). Interacts (via sigma-70 factor domain 4) with the phage G1 protein gp67; this inhibits rRNA synthesis. Interaction with phage G1 protein gp67 does not inhibit transcription in general, but selectively inhibits transcription from promoters that require interaction of the RNA polymerase alpha subunit with DNA sequences upstream of the -35 promoter element.1 PublicationUniRule annotation

Protein-protein interaction databases

STRINGi93061.SAOUHSC_01662.

Structurei

Secondary structure

1
368
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi297 – 3059
Helixi309 – 31911
Turni320 – 3234
Helixi329 – 3368
Helixi340 – 35516

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4G6DX-ray2.00A297-368[»]
4G8XX-ray3.00A/C297-357[»]
4G94X-ray2.00A297-357[»]
ProteinModelPortaliP0A0J0.
SMRiP0A0J0. Positions 24-368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni16 – 9075Sigma-70 factor domain-1Add
BLAST
Regioni135 – 20571Sigma-70 factor domain-2Add
BLAST
Regioni214 – 29178Sigma-70 factor domain-3Add
BLAST
Regioni303 – 35654Sigma-70 factor domain-4Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi159 – 1624Interaction with polymerase core subunit RpoC

Domaini

In the autoinhibited state, sigma-70 factor domain-1 packs closely together with sigma-70 factor domains-2 and -4, contrary to the extended conformation that is seen when the protein is part of the RNA polymerase holoenzyme.By similarity
The sigma-70 factor domain-2 mediates sequence-specific interaction with the -10 element in promoter DNA, and plays an important role in melting the double-stranded DNA and the formation of the transcription bubble. The sigma-70 factor domain-2 mediates interaction with the RNA polymerase subunits RpoB and RpoC (By similarity).By similarity
The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-H) motif that mediates interaction with the -35 element in promoter DNA. The domain also mediates interaction with the RNA polymerase subunit RpoA. Interactions between sigma-70 factor domain-4 and anti-sigma factors prevents interaction of sigma factors with the RNA polymerase catalytic core (By similarity).By similarity

Sequence similaritiesi

Belongs to the sigma-70 factor family. RpoD/SigA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0568.
HOGENOMiHOG000270273.
KOiK03086.
OMAiDMDLTPE.
OrthoDBiEOG6XHC70.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
HAMAPiMF_00963. Sigma70_RpoD_SigA.
InterProiIPR014284. RNA_pol_sigma-70_dom.
IPR000943. RNA_pol_sigma70.
IPR009042. RNA_pol_sigma70_r1_2.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR007127. RNA_pol_sigma_70_r1_1.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR012760. RNA_pol_sigma_RpoD_C.
IPR028630. Sigma70_RpoD.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF03979. Sigma70_r1_1. 1 hit.
PF00140. Sigma70_r1_2. 1 hit.
PF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PRINTSiPR00046. SIGMA70FCT.
SUPFAMiSSF88659. SSF88659. 2 hits.
SSF88946. SSF88946. 1 hit.
TIGRFAMsiTIGR02393. RpoD_Cterm. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
PROSITEiPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A0J0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDNTVKIKK QTIDPTLTLE DVKKQLIEKG KKEGHLSHEE IAEKLQNFDI
60 70 80 90 100
DSDQMDDFFD QLNDNDISLV NEKDSSDTDE KLNPSDLSAP PGVKINDPVR
110 120 130 140 150
MYLKEIGRVN LLSAQEEIEL AKRIEQGDEV AKSRLAEANL RLVVSIAKRY
160 170 180 190 200
VGRGMLFLDL IQEGNMGLIK AVEKFDFNKG FKFSTYATWW IRQAITRAIA
210 220 230 240 250
DQARTIRIPV HMVETINKLI RVQRQLLQDL GRDPAPEEIG EEMDLPAEKV
260 270 280 290 300
REILKIAQEP VSLETPIGEE DDSHLGDFIE DQEAQSPSDH AAYELLKEQL
310 320 330 340 350
EDVLDTLTDR EENVLRLRFG LDDGRTRTLE EVGKVFGVTR ERIRQIEAKA
360
LRKLRHPSRS KRLKDFMD
Length:368
Mass (Da):42,171
Last modified:March 1, 2005 - v1
Checksum:iAB029CA4F5A93A55
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti209 – 2091P → S in plaC1 mutation; results in a narrower specificity for promoters.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63177 Genomic DNA. Translation: AAB59090.1.
CP000253 Genomic DNA. Translation: ABD30737.1.
PIRiS34442.
RefSeqiWP_001283055.1. NC_007795.1.
YP_500173.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD30737; ABD30737; SAOUHSC_01662.
GeneIDi3920074.
KEGGisao:SAOUHSC_01662.
PATRICi19580705. VBIStaAur99865_1512.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63177 Genomic DNA. Translation: AAB59090.1 .
CP000253 Genomic DNA. Translation: ABD30737.1 .
PIRi S34442.
RefSeqi WP_001283055.1. NC_007795.1.
YP_500173.1. NC_007795.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4G6D X-ray 2.00 A 297-368 [» ]
4G8X X-ray 3.00 A/C 297-357 [» ]
4G94 X-ray 2.00 A 297-357 [» ]
ProteinModelPortali P0A0J0.
SMRi P0A0J0. Positions 24-368.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 93061.SAOUHSC_01662.

Proteomic databases

PRIDEi P0A0J0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD30737 ; ABD30737 ; SAOUHSC_01662 .
GeneIDi 3920074.
KEGGi sao:SAOUHSC_01662.
PATRICi 19580705. VBIStaAur99865_1512.

Phylogenomic databases

eggNOGi COG0568.
HOGENOMi HOG000270273.
KOi K03086.
OMAi DMDLTPE.
OrthoDBi EOG6XHC70.

Enzyme and pathway databases

BioCyci SAUR93061:GIWJ-1587-MONOMER.

Family and domain databases

Gene3Di 1.10.10.10. 2 hits.
HAMAPi MF_00963. Sigma70_RpoD_SigA.
InterProi IPR014284. RNA_pol_sigma-70_dom.
IPR000943. RNA_pol_sigma70.
IPR009042. RNA_pol_sigma70_r1_2.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR007127. RNA_pol_sigma_70_r1_1.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR012760. RNA_pol_sigma_RpoD_C.
IPR028630. Sigma70_RpoD.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF03979. Sigma70_r1_1. 1 hit.
PF00140. Sigma70_r1_2. 1 hit.
PF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view ]
PRINTSi PR00046. SIGMA70FCT.
SUPFAMi SSF88659. SSF88659. 2 hits.
SSF88946. SSF88946. 1 hit.
TIGRFAMsi TIGR02393. RpoD_Cterm. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
PROSITEi PS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Staphylococcus aureus chromosomal gene plaC, identified by mutations amplifying plasmid pT181, encodes a sigma factor."
    Basheer R., Iordanescu S.
    Nucleic Acids Res. 19:4921-4924(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The Staphylococcus aureus NCTC 8325 genome."
    Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.
    (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.); Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington D.C. (2006)
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 8325.
  3. "Promoter-specific transcription inhibition in Staphylococcus aureus by a phage protein."
    Osmundson J., Montero-Diez C., Westblade L.F., Hochschild A., Darst S.A.
    Cell 151:1005-1016(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 297-368 IN COMPLEX WITH PHAGE PROTEIN GP67, FUNCTION, SUBUNIT.

Entry informationi

Entry nameiSIGA_STAA8
AccessioniPrimary (citable) accession number: P0A0J0
Secondary accession number(s): P26766, Q2FY12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3