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Protein

RNA polymerase sigma factor SigA

Gene

sigA

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.UniRule annotation1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi329 – 348H-T-H motifUniRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Sigma factor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciSAUR93061:G1G5Y-1545-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma factor SigAUniRule annotation
Gene namesi
Name:sigAUniRule annotation
Synonyms:plaC, rpoD
Ordered Locus Names:SAOUHSC_01662
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000939161 – 368RNA polymerase sigma factor SigAAdd BLAST368

Interactioni

Subunit structurei

Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription (By similarity). Interacts (via sigma-70 factor domain 4) with the phage G1 protein gp67; this inhibits rRNA synthesis. Interaction with phage G1 protein gp67 does not inhibit transcription in general, but selectively inhibits transcription from promoters that require interaction of the RNA polymerase alpha subunit with DNA sequences upstream of the -35 promoter element.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi93061.SAOUHSC_01662

Structurei

Secondary structure

1368
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi297 – 305Combined sources9
Helixi309 – 319Combined sources11
Turni320 – 323Combined sources4
Helixi329 – 336Combined sources8
Helixi340 – 355Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4G6DX-ray2.00A297-368[»]
4G8XX-ray3.00A/C297-357[»]
4G94X-ray2.00A297-357[»]
ProteinModelPortaliP0A0J0
SMRiP0A0J0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni16 – 90Sigma-70 factor domain-1Add BLAST75
Regioni135 – 205Sigma-70 factor domain-2Add BLAST71
Regioni214 – 291Sigma-70 factor domain-3Add BLAST78
Regioni303 – 356Sigma-70 factor domain-4Add BLAST54

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi159 – 162Interaction with polymerase core subunit RpoC4

Domaini

In the autoinhibited state, sigma-70 factor domain-1 packs closely together with sigma-70 factor domains-2 and -4, contrary to the extended conformation that is seen when the protein is part of the RNA polymerase holoenzyme.By similarity
The sigma-70 factor domain-2 mediates sequence-specific interaction with the -10 element in promoter DNA, and plays an important role in melting the double-stranded DNA and the formation of the transcription bubble. The sigma-70 factor domain-2 mediates interaction with the RNA polymerase subunits RpoB and RpoC (By similarity).By similarity
The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-H) motif that mediates interaction with the -35 element in promoter DNA. The domain also mediates interaction with the RNA polymerase subunit RpoA. Interactions between sigma-70 factor domain-4 and anti-sigma factors prevents interaction of sigma factors with the RNA polymerase catalytic core (By similarity).By similarity

Sequence similaritiesi

Belongs to the sigma-70 factor family. RpoD/SigA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DG1 Bacteria
COG0568 LUCA
HOGENOMiHOG000270273
KOiK03086
OMAiYQRWLAE

Family and domain databases

Gene3Di1.10.10.10, 2 hits
HAMAPiMF_00963 Sigma70_RpoD_SigA, 1 hit
InterProiView protein in InterPro
IPR014284 RNA_pol_sigma-70_dom
IPR000943 RNA_pol_sigma70
IPR009042 RNA_pol_sigma70_r1_2
IPR007627 RNA_pol_sigma70_r2
IPR007624 RNA_pol_sigma70_r3
IPR007630 RNA_pol_sigma70_r4
IPR007127 RNA_pol_sigma_70_r1_1
IPR013325 RNA_pol_sigma_r2
IPR013324 RNA_pol_sigma_r3/r4-like
IPR012760 RNA_pol_sigma_RpoD_C
IPR028630 Sigma70_RpoD
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF03979 Sigma70_r1_1, 1 hit
PF00140 Sigma70_r1_2, 1 hit
PF04542 Sigma70_r2, 1 hit
PF04539 Sigma70_r3, 1 hit
PF04545 Sigma70_r4, 1 hit
PRINTSiPR00046 SIGMA70FCT
SUPFAMiSSF88659 SSF88659, 2 hits
SSF88946 SSF88946, 1 hit
TIGRFAMsiTIGR02393 RpoD_Cterm, 1 hit
TIGR02937 sigma70-ECF, 1 hit
PROSITEiView protein in PROSITE
PS00715 SIGMA70_1, 1 hit
PS00716 SIGMA70_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0A0J0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDNTVKIKK QTIDPTLTLE DVKKQLIEKG KKEGHLSHEE IAEKLQNFDI
60 70 80 90 100
DSDQMDDFFD QLNDNDISLV NEKDSSDTDE KLNPSDLSAP PGVKINDPVR
110 120 130 140 150
MYLKEIGRVN LLSAQEEIEL AKRIEQGDEV AKSRLAEANL RLVVSIAKRY
160 170 180 190 200
VGRGMLFLDL IQEGNMGLIK AVEKFDFNKG FKFSTYATWW IRQAITRAIA
210 220 230 240 250
DQARTIRIPV HMVETINKLI RVQRQLLQDL GRDPAPEEIG EEMDLPAEKV
260 270 280 290 300
REILKIAQEP VSLETPIGEE DDSHLGDFIE DQEAQSPSDH AAYELLKEQL
310 320 330 340 350
EDVLDTLTDR EENVLRLRFG LDDGRTRTLE EVGKVFGVTR ERIRQIEAKA
360
LRKLRHPSRS KRLKDFMD
Length:368
Mass (Da):42,171
Last modified:March 1, 2005 - v1
Checksum:iAB029CA4F5A93A55
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti209P → S in plaC1 mutation; results in a narrower specificity for promoters. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63177 Genomic DNA Translation: AAB59090.1
CP000253 Genomic DNA Translation: ABD30737.1
PIRiS34442
RefSeqiWP_001283055.1, NC_007795.1
YP_500173.1, NC_007795.1

Genome annotation databases

EnsemblBacteriaiABD30737; ABD30737; SAOUHSC_01662
GeneIDi3920074
KEGGisao:SAOUHSC_01662
PATRICifig|93061.5.peg.1512

Similar proteinsi

Entry informationi

Entry nameiSIGA_STAA8
AccessioniPrimary (citable) accession number: P0A0J0
Secondary accession number(s): P26766, Q2FY12
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: April 25, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health