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P0A0J0

- SIGA_STAA8

UniProt

P0A0J0 - SIGA_STAA8

Protein

RNA polymerase sigma factor SigA

Gene

sigA

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Mar 2005)
      Previous versions | rss
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    Functioni

    Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.1 PublicationUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi329 – 34820H-T-H motifUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-HAMAP
    2. sequence-specific DNA binding transcription factor activity Source: InterPro
    3. sigma factor activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. transcription initiation from bacterial-type RNA polymerase promoter Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Sigma factor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciSAUR93061:GIWJ-1587-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA polymerase sigma factor SigAUniRule annotation
    Gene namesi
    Name:sigAUniRule annotation
    Synonyms:plaC, rpoD
    Ordered Locus Names:SAOUHSC_01662
    OrganismiStaphylococcus aureus (strain NCTC 8325)
    Taxonomic identifieri93061 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000008816: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 368368RNA polymerase sigma factor SigAPRO_0000093916Add
    BLAST

    Proteomic databases

    PRIDEiP0A0J0.

    Interactioni

    Subunit structurei

    Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription By similarity. Interacts (via sigma-70 factor domain 4) with the phage G1 protein gp67; this inhibits rRNA synthesis. Interaction with phage G1 protein gp67 does not inhibit transcription in general, but selectively inhibits transcription from promoters that require interaction of the RNA polymerase alpha subunit with DNA sequences upstream of the -35 promoter element.1 PublicationUniRule annotation

    Protein-protein interaction databases

    STRINGi93061.SAOUHSC_01662.

    Structurei

    Secondary structure

    1
    368
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi297 – 3059
    Helixi309 – 31911
    Turni320 – 3234
    Helixi329 – 3368
    Helixi340 – 35516

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4G6DX-ray2.00A297-368[»]
    4G8XX-ray3.00A/C297-357[»]
    4G94X-ray2.00A297-357[»]
    ProteinModelPortaliP0A0J0.
    SMRiP0A0J0. Positions 24-368.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni16 – 9075Sigma-70 factor domain-1Add
    BLAST
    Regioni135 – 20571Sigma-70 factor domain-2Add
    BLAST
    Regioni214 – 29178Sigma-70 factor domain-3Add
    BLAST
    Regioni303 – 35654Sigma-70 factor domain-4Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi159 – 1624Interaction with polymerase core subunit RpoC

    Domaini

    In the autoinhibited state, sigma-70 factor domain-1 packs closely together with sigma-70 factor domains-2 and -4, contrary to the extended conformation that is seen when the protein is part of the RNA polymerase holoenzyme.By similarity
    The sigma-70 factor domain-2 mediates sequence-specific interaction with the -10 element in promoter DNA, and plays an important role in melting the double-stranded DNA and the formation of the transcription bubble. The sigma-70 factor domain-2 mediates interaction with the RNA polymerase subunits RpoB and RpoC By similarity.By similarity
    The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-H) motif that mediates interaction with the -35 element in promoter DNA. The domain also mediates interaction with the RNA polymerase subunit RpoA. Interactions between sigma-70 factor domain-4 and anti-sigma factors prevents interaction of sigma factors with the RNA polymerase catalytic core By similarity.By similarity

    Sequence similaritiesi

    Belongs to the sigma-70 factor family. RpoD/SigA subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0568.
    HOGENOMiHOG000270273.
    KOiK03086.
    OMAiDMDLTPE.
    OrthoDBiEOG6XHC70.

    Family and domain databases

    Gene3Di1.10.10.10. 2 hits.
    HAMAPiMF_00963. Sigma70_RpoD_SigA.
    InterProiIPR014284. RNA_pol_sigma-70_dom.
    IPR000943. RNA_pol_sigma70.
    IPR009042. RNA_pol_sigma70_r1_2.
    IPR007627. RNA_pol_sigma70_r2.
    IPR007624. RNA_pol_sigma70_r3.
    IPR007630. RNA_pol_sigma70_r4.
    IPR007127. RNA_pol_sigma_70_r1_1.
    IPR013325. RNA_pol_sigma_r2.
    IPR013324. RNA_pol_sigma_r3_r4.
    IPR012760. RNA_pol_sigma_RpoD_C.
    IPR028630. Sigma70_RpoD.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF03979. Sigma70_r1_1. 1 hit.
    PF00140. Sigma70_r1_2. 1 hit.
    PF04542. Sigma70_r2. 1 hit.
    PF04539. Sigma70_r3. 1 hit.
    PF04545. Sigma70_r4. 1 hit.
    [Graphical view]
    PRINTSiPR00046. SIGMA70FCT.
    SUPFAMiSSF88659. SSF88659. 2 hits.
    SSF88946. SSF88946. 1 hit.
    TIGRFAMsiTIGR02393. RpoD_Cterm. 1 hit.
    TIGR02937. sigma70-ECF. 1 hit.
    PROSITEiPS00715. SIGMA70_1. 1 hit.
    PS00716. SIGMA70_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A0J0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDNTVKIKK QTIDPTLTLE DVKKQLIEKG KKEGHLSHEE IAEKLQNFDI    50
    DSDQMDDFFD QLNDNDISLV NEKDSSDTDE KLNPSDLSAP PGVKINDPVR 100
    MYLKEIGRVN LLSAQEEIEL AKRIEQGDEV AKSRLAEANL RLVVSIAKRY 150
    VGRGMLFLDL IQEGNMGLIK AVEKFDFNKG FKFSTYATWW IRQAITRAIA 200
    DQARTIRIPV HMVETINKLI RVQRQLLQDL GRDPAPEEIG EEMDLPAEKV 250
    REILKIAQEP VSLETPIGEE DDSHLGDFIE DQEAQSPSDH AAYELLKEQL 300
    EDVLDTLTDR EENVLRLRFG LDDGRTRTLE EVGKVFGVTR ERIRQIEAKA 350
    LRKLRHPSRS KRLKDFMD 368
    Length:368
    Mass (Da):42,171
    Last modified:March 1, 2005 - v1
    Checksum:iAB029CA4F5A93A55
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti209 – 2091P → S in plaC1 mutation; results in a narrower specificity for promoters.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63177 Genomic DNA. Translation: AAB59090.1.
    CP000253 Genomic DNA. Translation: ABD30737.1.
    PIRiS34442.
    RefSeqiWP_001283055.1. NC_007795.1.
    YP_500173.1. NC_007795.1.

    Genome annotation databases

    EnsemblBacteriaiABD30737; ABD30737; SAOUHSC_01662.
    GeneIDi3920074.
    KEGGisao:SAOUHSC_01662.
    PATRICi19580705. VBIStaAur99865_1512.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63177 Genomic DNA. Translation: AAB59090.1 .
    CP000253 Genomic DNA. Translation: ABD30737.1 .
    PIRi S34442.
    RefSeqi WP_001283055.1. NC_007795.1.
    YP_500173.1. NC_007795.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4G6D X-ray 2.00 A 297-368 [» ]
    4G8X X-ray 3.00 A/C 297-357 [» ]
    4G94 X-ray 2.00 A 297-357 [» ]
    ProteinModelPortali P0A0J0.
    SMRi P0A0J0. Positions 24-368.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 93061.SAOUHSC_01662.

    Proteomic databases

    PRIDEi P0A0J0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABD30737 ; ABD30737 ; SAOUHSC_01662 .
    GeneIDi 3920074.
    KEGGi sao:SAOUHSC_01662.
    PATRICi 19580705. VBIStaAur99865_1512.

    Phylogenomic databases

    eggNOGi COG0568.
    HOGENOMi HOG000270273.
    KOi K03086.
    OMAi DMDLTPE.
    OrthoDBi EOG6XHC70.

    Enzyme and pathway databases

    BioCyci SAUR93061:GIWJ-1587-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.10. 2 hits.
    HAMAPi MF_00963. Sigma70_RpoD_SigA.
    InterProi IPR014284. RNA_pol_sigma-70_dom.
    IPR000943. RNA_pol_sigma70.
    IPR009042. RNA_pol_sigma70_r1_2.
    IPR007627. RNA_pol_sigma70_r2.
    IPR007624. RNA_pol_sigma70_r3.
    IPR007630. RNA_pol_sigma70_r4.
    IPR007127. RNA_pol_sigma_70_r1_1.
    IPR013325. RNA_pol_sigma_r2.
    IPR013324. RNA_pol_sigma_r3_r4.
    IPR012760. RNA_pol_sigma_RpoD_C.
    IPR028630. Sigma70_RpoD.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF03979. Sigma70_r1_1. 1 hit.
    PF00140. Sigma70_r1_2. 1 hit.
    PF04542. Sigma70_r2. 1 hit.
    PF04539. Sigma70_r3. 1 hit.
    PF04545. Sigma70_r4. 1 hit.
    [Graphical view ]
    PRINTSi PR00046. SIGMA70FCT.
    SUPFAMi SSF88659. SSF88659. 2 hits.
    SSF88946. SSF88946. 1 hit.
    TIGRFAMsi TIGR02393. RpoD_Cterm. 1 hit.
    TIGR02937. sigma70-ECF. 1 hit.
    PROSITEi PS00715. SIGMA70_1. 1 hit.
    PS00716. SIGMA70_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Staphylococcus aureus chromosomal gene plaC, identified by mutations amplifying plasmid pT181, encodes a sigma factor."
      Basheer R., Iordanescu S.
      Nucleic Acids Res. 19:4921-4924(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The Staphylococcus aureus NCTC 8325 genome."
      Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.
      (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.); Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington D.C. (2006)
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NCTC 8325.
    3. "Promoter-specific transcription inhibition in Staphylococcus aureus by a phage protein."
      Osmundson J., Montero-Diez C., Westblade L.F., Hochschild A., Darst S.A.
      Cell 151:1005-1016(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 297-368 IN COMPLEX WITH PHAGE PROTEIN GP67, FUNCTION, SUBUNIT.

    Entry informationi

    Entry nameiSIGA_STAA8
    AccessioniPrimary (citable) accession number: P0A0J0
    Secondary accession number(s): P26766, Q2FY12
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3