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P0A0H9

- FABG_STAAM

UniProt

P0A0H9 - FABG_STAAM

Protein

3-oxoacyl-[acyl-carrier-protein] reductase FabG

Gene

fabG

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (01 Mar 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.By similarity

    Catalytic activityi

    (3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei89 – 891NADP; via carbonyl oxygenBy similarity
    Binding sitei141 – 1411SubstrateBy similarity
    Active sitei154 – 1541Proton acceptorPROSITE-ProRule annotation
    Binding sitei187 – 1871NADP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 144NADPBy similarity
    Nucleotide bindingi62 – 632NADPBy similarity
    Nucleotide bindingi154 – 1585NADPBy similarity

    GO - Molecular functioni

    1. 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Source: UniProtKB-EC
    2. NAD binding Source: InterPro

    GO - Biological processi

    1. fatty acid biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciSAUR158878:GJJ5-1249-MONOMER.
    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-oxoacyl-[acyl-carrier-protein] reductase FabG (EC:1.1.1.100)
    Alternative name(s):
    3-ketoacyl-acyl carrier protein reductase
    Beta-Ketoacyl-acyl carrier protein reductase
    Beta-ketoacyl-ACP reductase
    Gene namesi
    Name:fabG
    Ordered Locus Names:SAV1231
    OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
    Taxonomic identifieri158878 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000002481: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2462463-oxoacyl-[acyl-carrier-protein] reductase FabGPRO_0000054684Add
    BLAST

    2D gel databases

    World-2DPAGE0002:P0A0H9.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi158878.SAV1231.

    Structurei

    Secondary structure

    1
    246
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 94
    Helixi15 – 2612
    Beta strandi30 – 378
    Helixi39 – 5113
    Beta strandi56 – 605
    Helixi66 – 8015
    Beta strandi85 – 884
    Turni98 – 1003
    Helixi103 – 11311
    Helixi115 – 13117
    Beta strandi134 – 1396
    Helixi142 – 1465
    Helixi152 – 17221
    Helixi173 – 1753
    Beta strandi177 – 1848
    Helixi188 – 1903
    Helixi197 – 2048
    Helixi215 – 22511
    Helixi228 – 2303
    Beta strandi237 – 2415

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3OSUX-ray1.90A/B1-246[»]
    ProteinModelPortaliP0A0H9.
    SMRiP0A0H9. Positions 1-246.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1028.
    KOiK00059.
    OMAiDEFGAID.
    OrthoDBiEOG6N3CR8.
    PhylomeDBiP0A0H9.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR011284. 3oxo_ACP_reduc.
    IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000126. 11-beta-HSD1. 1 hit.
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A0H9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKMTKSALVT GASRGIGRSI ALQLAEEGYN VAVNYAGSKE KAEAVVEEIK    50
    AKGVDSFAIQ ANVADADEVK AMIKEVVSQF GSLDVLVNNA GITRDNLLMR 100
    MKEQEWDDVI DTNLKGVFNC IQKATPQMLR QRSGAIINLS SVVGAVGNPG 150
    QANYVATKAG VIGLTKSAAR ELASRGITVN AVAPGFIVSD MTDALSDELK 200
    EQMLTQIPLA RFGQDTDIAN TVAFLASDKA KYITGQTIHV NGGMYM 246
    Length:246
    Mass (Da):26,146
    Last modified:March 1, 2005 - v1
    Checksum:i2BD7CA319D3A37C5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000017 Genomic DNA. Translation: BAB57393.1.
    RefSeqiNP_371755.1. NC_002758.2.

    Genome annotation databases

    EnsemblBacteriaiBAB57393; BAB57393; SAV1231.
    GeneIDi1121208.
    KEGGisav:SAV1231.
    PATRICi19563175. VBIStaAur52173_1265.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000017 Genomic DNA. Translation: BAB57393.1 .
    RefSeqi NP_371755.1. NC_002758.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3OSU X-ray 1.90 A/B 1-246 [» ]
    ProteinModelPortali P0A0H9.
    SMRi P0A0H9. Positions 1-246.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 158878.SAV1231.

    2D gel databases

    World-2DPAGE 0002:P0A0H9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB57393 ; BAB57393 ; SAV1231 .
    GeneIDi 1121208.
    KEGGi sav:SAV1231.
    PATRICi 19563175. VBIStaAur52173_1265.

    Phylogenomic databases

    eggNOGi COG1028.
    KOi K00059.
    OMAi DEFGAID.
    OrthoDBi EOG6N3CR8.
    PhylomeDBi P0A0H9.

    Enzyme and pathway databases

    UniPathwayi UPA00094 .
    BioCyci SAUR158878:GJJ5-1249-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR011284. 3oxo_ACP_reduc.
    IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000126. 11-beta-HSD1. 1 hit.
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    TIGRFAMsi TIGR01830. 3oxo_ACP_reduc. 1 hit.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Mu50 / ATCC 700699.
    2. "Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, FabG, from Staphylococcus aureus."
      Anderson S.M., Wawrzak Z., Onopriyenko O., Edwards A., Anderson W.F., Savchenko A.
      Submitted (SEP-2010) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS), SUBUNIT.

    Entry informationi

    Entry nameiFABG_STAAM
    AccessioniPrimary (citable) accession number: P0A0H9
    Secondary accession number(s): Q99QK7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3