P0A0H5 (RNPA_STAAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease P protein component
Short name=RNase P protein
Short name=RNaseP protein
EC=3.1.26.5

Alternative name(s):
Protein C5

Gene names

Name:

rnpA

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus

Protein attributes

Sequence length	117 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme. HAMAP-Rule MF_00227
Catalytic activity	Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor. HAMAP-Rule MF_00227
Subunit structure	Consists of a catalytic RNA component (M1 or RnpB) and a protein subunit.
Sequence similarities	Belongs to the RnpA family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA 5'-leader removal Inferred from electronic annotation. Source: HAMAP
Molecular_function	ribonuclease P activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 117

117

Ribonuclease P protein component HAMAP-Rule MF_00227

PRO_0000198530

Secondary structure

1

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117

Helix Strand Turn

Details...

Helix

5 – 7

3

Helix

13 – 21

9

Beta strand

27 – 31

5

Beta strand

45 – 48

4

Beta strand

51 – 53

3

Helix

58 – 72

15

Helix

73 – 75

3

Beta strand

82 – 86

5

Helix

89 – 92

4

Helix

97 – 102

6

Helix

105 – 109

5

Beta strand

112 – 114

3

Sequences

	Sequence		Length	Mass (Da)
	P0A0H5 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: AD5172C3654E01F0	FASTA	117	13,652
	10 20 30 40 50 60 MLLEKAYRIK KNADFQRIYK KGHSVANRQF VVYTCNNKEI DHFRLGISVS KKLGNAVLRN 70 80 90 100 110 KIKRAIRENF KVHKSHILAK DIIVIARQPA KDMTTLQIQN SLEHVLKIAK VFNKKIK

References

[1]	"Cloning and characterization of Staphylococcus aureus ribonuclease P." Poppe S.M., Swaney S.M., Choi G.H., Hromockyj A., Shinabarger D.L. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ISP3.
[2]	"The structure of ribonuclease P protein from Staphylococcus aureus reveals a unique binding site for single-stranded RNA." Spitzfaden C., Nicholson N., Jones J.J., Guth S., Lehr R., Prescott C.D., Hegg L.A., Eggleston D.S. J. Mol. Biol. 295:105-115(2000) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF135268 Genomic DNA. Translation: AAF61418.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1D6T	NMR	-	A	1-117	[»]

ProteinModelPortal

P0A0H5.

SMR

P0A0H5. Positions 1-117.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.30.230.10. 1 hit.

HAMAP

MF_00227. RNase_P.

InterPro

IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR000100. RNase_P.
IPR020539. RNase_P_CS.
[Graphical view]

Pfam

PF00825. Ribonuclease_P. 1 hit.
[Graphical view]

ProDom

PD003629. Ribonuclease_P. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.

TIGRFAMs

TIGR00188. rnpA. 1 hit.

PROSITE

PS00648. RIBONUCLEASE_P. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0A0H5.

Entry information

Entry name

RNPA_STAAU

Accession

Primary (citable) accession number: P0A0H5
Secondary accession number(s): P58031, Q9LAH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	February 15, 2005
Last modified:	April 3, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program