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Protein

rRNA adenine N-6-methyltransferase

Gene

ermA

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This protein produces a dimethylation of the adenine residue at position 2085 in 23S rRNA, resulting in reduced affinity between ribosomes and macrolide-lincosamide-streptogramin B antibiotics.

Catalytic activityi

2 S-adenosyl-L-methionine + adenine(2085) in 23S rRNA = 2 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(2085) in 23S rRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei13 – 131S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
Binding sitei38 – 381S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei59 – 591S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei84 – 841S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei101 – 1011S-adenosyl-L-methioninePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
rRNA adenine N-6-methyltransferase (EC:2.1.1.184)
Alternative name(s):
Erythromycin resistance protein
Macrolide-lincosamide-streptogramin B resistance protein
Gene namesi
Name:ermA
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 243243rRNA adenine N-6-methyltransferasePRO_0000101681Add
BLAST

Proteomic databases

PaxDbiP0A0H3.
PRIDEiP0A0H3.

Interactioni

Protein-protein interaction databases

STRINGi158878.SAV1655.

Structurei

3D structure databases

ProteinModelPortaliP0A0H3.
SMRiP0A0H3. Positions 10-243.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG41071EZ. Bacteria.
COG0030. LUCA.
KOiK00561.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A0H3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQKNPKDTQ NFITSKKHVK EILNHTNISK QDNVIEIGSG KGHFTKELVK
60 70 80 90 100
MSRSVTAIEI DGGLCQVTKE AVNPSENIKV IQTDILKFSF PKHINYKIYG
110 120 130 140 150
NIPYNISTDI VKRITFESQA KYSYLIVEKG FAKRLQNLQR ALGLLLMVEM
160 170 180 190 200
DIKMLKKVPP LYFHPKPSVD SVLIVLERHQ PLISKKDYKK YRSFVYKWVN
210 220 230 240
REYRVLFTKN QFRQALKHAN VTNINKLSKE QFLSIFNSYK LFH
Length:243
Mass (Da):28,408
Last modified:January 1, 1988 - v1
Checksum:i03DDAFABE867525A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03216 Genomic DNA. Translation: CAA26964.1.
PIRiE24584. A25101.
RefSeqiWP_001072201.1. NZ_LSBC01000057.1.
YP_006958113.1. NC_019144.1.

Genome annotation databases

GeneIDi13913675.
KEGGiag:CAA26964.
pg:13913675.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03216 Genomic DNA. Translation: CAA26964.1.
PIRiE24584. A25101.
RefSeqiWP_001072201.1. NZ_LSBC01000057.1.
YP_006958113.1. NC_019144.1.

3D structure databases

ProteinModelPortaliP0A0H3.
SMRiP0A0H3. Positions 10-243.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi158878.SAV1655.

Proteomic databases

PaxDbiP0A0H3.
PRIDEiP0A0H3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13913675.
KEGGiag:CAA26964.
pg:13913675.

Phylogenomic databases

eggNOGiENOG41071EZ. Bacteria.
COG0030. LUCA.
KOiK00561.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Transposon Tn554: complete nucleotide sequence and isolation of transposition-defective and antibiotic-sensitive mutants."
    Murphy E., Huwyler L., Do Carno de Freire Bastos M.
    EMBO J. 4:3357-3365(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Transposon: Tn554.
  2. "Nucleotide sequence of ermA, a macrolide-lincosamide-streptogramin B determinant in Staphylococcus aureus."
    Murphy E.
    J. Bacteriol. 162:633-640(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiERMA_STAAU
AccessioniPrimary (citable) accession number: P0A0H3
Secondary accession number(s): P06699
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: July 6, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Transposable element

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.