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Protein

rRNA adenine N-6-methyltransferase

Gene

ermA1

more
Organism
Staphylococcus aureus (strain N315)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein produces a dimethylation of the adenine residue at position 2085 in 23S rRNA, resulting in reduced affinity between ribosomes and macrolide-lincosamide-streptogramin B antibiotics.By similarity

Catalytic activityi

2 S-adenosyl-L-methionine + adenine(2085) in 23S rRNA = 2 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(2085) in 23S rRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei13 – 131S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
Binding sitei38 – 381S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei59 – 591S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei84 – 841S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei101 – 1011S-adenosyl-L-methioninePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSAUR158879:GJCB-1553-MONOMER.
SAUR158879:GJCB-2084-MONOMER.
SAUR158879:GJCB-2547-MONOMER.
SAUR158879:GJCB-52-MONOMER.
SAUR158879:GJCB-808-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
rRNA adenine N-6-methyltransferase (EC:2.1.1.184)
Alternative name(s):
Erythromycin resistance protein
Macrolide-lincosamide-streptogramin B resistance protein
Gene namesi
Name:ermA1
Ordered Locus Names:SA0048
AND
Name:ermA2
Ordered Locus Names:SA1480
AND
Name:ermA3
Ordered Locus Names:SA0766
AND
Name:ermA4
Ordered Locus Names:SA1951
AND
Name:ermA5
Ordered Locus Names:SA2384
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000751 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 243243rRNA adenine N-6-methyltransferasePRO_0000101679Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP0A0H2.
SMRiP0A0H2. Positions 10-243.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000279685.
KOiK00561.
OMAiLMAEVDI.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A0H2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQKNPKDTQ NFITSKKHVK EILNHTNISK QDNVIEIGSG KGHFTKELVK
60 70 80 90 100
MSRSVTAIEI DGGLCQVTKE AVNPSENIKV IQTDILKFSF PKHINYKIYG
110 120 130 140 150
NIPYNISTDI VKRITFESQA KYSYLIVEKG FAKRLQNLQR ALGLLLMVEM
160 170 180 190 200
DIKMLKKVPP LYFHPKPSVD SVLIVLERHQ PLISKKDYKK YRSFVYKWVN
210 220 230 240
REYRVLFTKN QFRQALKHAN VTNINKLSKE QFLSIFNSYK LFH
Length:243
Mass (Da):28,408
Last modified:January 1, 1988 - v1
Checksum:i03DDAFABE867525A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86934 Genomic DNA. Translation: BAA82205.1.
BA000018 Genomic DNA. Translation: BAB41266.1.
BA000018 Genomic DNA. Translation: BAB42003.1.
BA000018 Genomic DNA. Translation: BAB42746.1.
BA000018 Genomic DNA. Translation: BAB43235.1.
BA000018 Genomic DNA. Translation: BAB43689.1.
RefSeqiWP_001072201.1. NC_002745.2.
YP_006958113.1. NC_019144.1.

Genome annotation databases

EnsemblBacteriaiBAB41266; BAB41266; BAB41266.
BAB42003; BAB42003; BAB42003.
BAB42746; BAB42746; BAB42746.
BAB43235; BAB43235; BAB43235.
BAB43689; BAB43689; BAB43689.
GeneIDi13913675.
KEGGipg:13913675.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86934 Genomic DNA. Translation: BAA82205.1.
BA000018 Genomic DNA. Translation: BAB41266.1.
BA000018 Genomic DNA. Translation: BAB42003.1.
BA000018 Genomic DNA. Translation: BAB42746.1.
BA000018 Genomic DNA. Translation: BAB43235.1.
BA000018 Genomic DNA. Translation: BAB43689.1.
RefSeqiWP_001072201.1. NC_002745.2.
YP_006958113.1. NC_019144.1.

3D structure databases

ProteinModelPortaliP0A0H2.
SMRiP0A0H2. Positions 10-243.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB41266; BAB41266; BAB41266.
BAB42003; BAB42003; BAB42003.
BAB42746; BAB42746; BAB42746.
BAB43235; BAB43235; BAB43235.
BAB43689; BAB43689; BAB43689.
GeneIDi13913675.
KEGGipg:13913675.

Phylogenomic databases

HOGENOMiHOG000279685.
KOiK00561.
OMAiLMAEVDI.

Enzyme and pathway databases

BioCyciSAUR158879:GJCB-1553-MONOMER.
SAUR158879:GJCB-2084-MONOMER.
SAUR158879:GJCB-2547-MONOMER.
SAUR158879:GJCB-52-MONOMER.
SAUR158879:GJCB-808-MONOMER.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERMA_STAAN
AccessioniPrimary (citable) accession number: P0A0H2
Secondary accession number(s): P06699
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: September 7, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.