P0A0F0 (RELA_STAAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP pyrophosphokinase
EC=2.7.6.5

Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I

Gene names

Name:	relA
Synonyms:	rel

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus

Protein attributes

Sequence length	736 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp By similarity.
Catalytic activity	ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.
Pathway	Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.
Sequence similarities	Belongs to the RelA/SpoT family. Contains 1 ACT domain. Contains 1 HD domain.

Ontologies

Keywords
Ligand	ATP-binding GTP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	guanosine tetraphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP diphosphokinase activity Inferred from electronic annotation. Source: EC amino acid binding Inferred from electronic annotation. Source: InterPro kinase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: InterPro phosphoric diester hydrolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 736	736	GTP pyrophosphokinase		PRO_0000166559
Regions
	Domain	57 – 156	100	HD
	Domain	661 – 734	74	ACT

Sequences

	Sequence		Length	Mass (Da)
	P0A0F0 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: C1EC881D00431801	FASTA	736	84,537
	10 20 30 40 50 60 MNGVYHIMNN EYPYSADEVL HKAKSYLSAD EYEYVLKSYH IAYEAHKGQF RKNGLPYIMH 70 80 90 100 110 120 PIQVAGILTE MRLDGPTIVA GFLHDVIEDT PYTFEDVKEM FNEEVARIVD GVTKLKKVKY 130 140 150 160 170 180 RSKEEQQAEN HRKLFIAIAK DVRVILVKLA DRLHNMRTLK AMPREKQIRI SRETLEIYAP 190 200 210 220 230 240 LAHRLGINTI KWELEDTALR YIDNVQYFRI VNLMKKKRSE REAYIETAID RIRTEMDRMN 250 260 270 280 290 300 IEGDINGRPK HIYSIYRKMM KQKKQFDQIF DLLAIRVIVN SINDCYAILG LVHTLWKPMP 310 320 330 340 350 360 GRFKDYIAMP KQNLYQSLHT TVVGPNGDPL EIQIRTFDMH EIAEHGVAAH WAYKEGKKVS 370 380 390 400 410 420 EKDQTYQNKL NWLKELAEAD HTSSDAQEFM ETLKYDLQSD KVYAFTPASD VIELPYGAVP 430 440 450 460 470 480 IDFAYAIHSE VGNKMIGAKV NGKIVPIDYI LQTGDIVEIR TSKHSYGPSR DWLKIVKSSS 490 500 510 520 530 540 AKGKIKSFFK KQDRSSNIEK GRMMVEVEIK EQGFRVEDIL TEKNIQVVNE KYNFANEDDL 550 560 570 580 590 600 FAAVGFGGVT SLQIVNKLTE RQRILDKQRA LNEAQEVTKS LPIKDNIITD SGVYVEGLEN 610 620 630 640 650 660 VLIKLSKCCN PIPGDDIVGY ITKGHGIKVH RTDCPNIKNE TERLINVEWV KSKDATQKYQ 670 680 690 700 710 720 VDLEVTAYDR NGLLNEVLQA VSSTAGNLIK VSGRSDIDKN AIINISVMVK NVNDVYRVVE 730 KIKQLGDVYT VTRVWN

References

[1]	"Increase of methicillin resistance in Staphylococcus aureus caused by deletion of a gene whose product is homologous to lytic enzymes." Fujimura T., Murakami K. J. Bacteriol. 179:6294-6301(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SR17238.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D76414 Genomic DNA. Translation: BAA23138.1.
3D structure databases
ProteinModelPortal	P0A0F0.
SMR	P0A0F0. Positions 14-357.
ModBase	Search...
Proteomic databases
PRIDE	P0A0F0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG0317.
Enzyme and pathway databases
UniPathway	UPA00908; UER00884.
Family and domain databases
Gene3D	3.10.20.30. 1 hit.
InterPro	IPR026020. (p)ppGpp_Synthase. IPR002912. ACT_dom. IPR012675. Beta-grasp_dom. IPR003607. HD/PDEase_dom. IPR006674. HD_domain. IPR004811. RelA/Spo_fam. IPR007685. RelA_SpoT. IPR004095. TGS. IPR012676. TGS-like. [Graphical view]
PANTHER	PTHR21262. PTHR21262. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF01966. HD. 1 hit. PF04607. RelA_SpoT. 1 hit. PF02824. TGS. 1 hit. [Graphical view]
SMART	SM00471. HDc. 1 hit. SM00954. RelA_SpoT. 1 hit. [Graphical view]
SUPFAM	SSF81271. TGS-like. 1 hit.
TIGRFAMs	TIGR00691. spoT_relA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RELA_STAAU

Accession

Primary (citable) accession number: P0A0F0
Secondary accession number(s): O32419

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	February 15, 2005
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program