Reviewed, UniProtKB/Swiss-Prot P0A0E2 (PTHP_STAAW)

Names and origin

Protein names

Recommended name:
    Phosphocarrier protein HPr
    EC=2.7.11.-
Alternative name(s):
    Histidine-containing protein

Gene names

Name:	ptsH
Ordered Locus Names:	MW0965

Organism

Staphylococcus aureus (strain MW2) [Complete proteome] [HAMAP]

Taxonomic identifier

196620 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	88 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein HPr by enzyme I. Phospho-HPr then transfers it to the permease (enzymes II/III) By similarity. P-Ser-HPr interacts with the catabolite control protein A (ccpA), forming a complex that binds to DNA at the catabolite response elements cre, operator sites preceding a large number of catabolite-regulated genes. Thus, P-Ser-HPr is a corepressor in carbon catabolite repression (CCR), a mechanism that allows bacteria to coordinate and optimize the utilization of available carbon sources. P-Ser-HPr also plays a role in inducer exclusion, in which it probably interacts with several non-PTS permeases and inhibits their transport activity By similarity.
Catalytic activity	Protein HPr N(pi)-phospho-L-histidine + protein EIIA = protein HPr + protein EIIA N(tau)-phospho-L-histidine.
Enzyme regulation	Phosphorylation on Ser-46 inhibits the phosphoryl transfer from enzyme I to HPr By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the HPr family. Contains 1 HPr domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transcription Transcription regulation Transport
Cellular component	Cytoplasm
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 88	88	Phosphocarrier protein HPr		PRO_0000107877
Regions
	Domain	1 – 88	88	HPr
Sites
	Active site	15	1	Pros-phosphohistidine intermediate By similarity
Amino acid modifications
	Modified residue	46	1	Phosphoserine; by HPrK/P By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A0E2-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: B2C6639D53226FF9	FASTA	88	9,496
	10 20 30 40 50 60 MEQNSYVIID ETGIHARPAT MLVQTASKFD SDIQLEYNGK KVNLKSIMGV MSLGVGKDAE 70 80 ITIYADGSDE SDAIQAISDV LSKEGLTK

References

[1]	"Genome and virulence determinants of high virulence community-acquired MRSA." Baba T., Takeuchi F., Kuroda M., Yuzawa H., Aoki K., Oguchi A., Nagai Y., Iwama N., Asano K., Naimi T., Kuroda H., Cui L., Yamamoto K., Hiramatsu K. Lancet 359:1819-1827(2002) [PubMed: 12044378] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000033 Genomic DNA. Translation: BAB94830.1.
RefSeq	NP_645782.1.
3D structure databases
SMR	P0A0E2. Positions 1-88.
ModBase	Search...
Protein-protein interaction databases
STRING	P0A0E2.
Genome annotation databases
GeneID	1003077.
GenomeReviews	Gene locus MW0965 in contig BA000033_GR.
KEGG	sam:MW0965.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG653254.
OMA	CDGDDEN.
Enzyme and pathway databases
BioCyc	SAUR196620:MW0965-MON.
Family and domain databases
InterPro	IPR001020. PTS_HPr_His_P_site. IPR005698. PTS_HPr_prot. IPR000032. PTS_HPr_prot-like. IPR002114. PTS_HPr_Ser_P_site. [Graphical view]
Gene3D	G3DSA:3.30.1340.10. PTS_HPr_protein. 1 hit.
Pfam	PF00381. PTS-HPr. 1 hit. [Graphical view]
PRINTS	PR00107. PHOSPHOCPHPR.
PROSITE	PS51350. PTS_HPR_DOM. 1 hit. PS00369. PTS_HPR_HIS. 1 hit. PS00589. PTS_HPR_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTHP_STAAW

Accession

Primary (citable) accession number: P0A0E2
Secondary accession number(s): P02907

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	March 1, 2005
Last modified:	December 15, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)