Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphocarrier protein HPr

Gene

ptsH

Organism
Staphylococcus aureus (strain MW2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein HPr by enzyme I. Phospho-HPr then transfers it to the permease (enzymes II/III) (By similarity).By similarity
P-Ser-HPr interacts with the catabolite control protein A (CcpA), forming a complex that binds to DNA at the catabolite response elements cre, operator sites preceding a large number of catabolite-regulated genes. Thus, P-Ser-HPr is a corepressor in carbon catabolite repression (CCR), a mechanism that allows bacteria to coordinate and optimize the utilization of available carbon sources. P-Ser-HPr also plays a role in inducer exclusion, in which it probably interacts with several non-PTS permeases and inhibits their transport activity (By similarity).By similarity

Catalytic activityi

Protein HPr N(pi)-phospho-L-histidine + protein EIIA = protein HPr + protein EIIA N(tau)-phospho-L-histidine.

Enzyme regulationi

Phosphorylation on Ser-46 inhibits the phosphoryl transfer from enzyme I to HPr.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei15Pros-phosphohistidine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transcription, Transcription regulation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphocarrier protein HPr (EC:2.7.11.-)
Alternative name(s):
Histidine-containing protein
Gene namesi
Name:ptsH
Ordered Locus Names:MW0965
OrganismiStaphylococcus aureus (strain MW2)
Taxonomic identifieri196620 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000418 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001078771 – 88Phosphocarrier protein HPrAdd BLAST88

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei46Phosphoserine; by HPrK/PPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliP0A0E2.
SMRiP0A0E2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 88HPrPROSITE-ProRule annotationAdd BLAST88

Sequence similaritiesi

Belongs to the HPr family.Curated

Phylogenomic databases

HOGENOMiHOG000278399.
KOiK11189.
OMAiAEVWVTR.

Family and domain databases

CDDicd00367. PTS-HPr_like. 1 hit.
Gene3Di3.30.1340.10. 1 hit.
InterProiView protein in InterPro
IPR000032. HPr-like.
IPR001020. PTS_HPr_His_P_site.
IPR002114. PTS_HPr_Ser_P_site.
PfamiView protein in Pfam
PF00381. PTS-HPr. 1 hit.
PRINTSiPR00107. PHOSPHOCPHPR.
SUPFAMiSSF55594. SSF55594. 1 hit.
TIGRFAMsiTIGR01003. PTS_HPr_family. 1 hit.
PROSITEiView protein in PROSITE
PS51350. PTS_HPR_DOM. 1 hit.
PS00369. PTS_HPR_HIS. 1 hit.
PS00589. PTS_HPR_SER. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A0E2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQNSYVIID ETGIHARPAT MLVQTASKFD SDIQLEYNGK KVNLKSIMGV
60 70 80
MSLGVGKDAE ITIYADGSDE SDAIQAISDV LSKEGLTK
Length:88
Mass (Da):9,496
Last modified:March 1, 2005 - v1
Checksum:iB2C6639D53226FF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA. Translation: BAB94830.1.
RefSeqiWP_000437472.1. NC_003923.1.

Genome annotation databases

EnsemblBacteriaiBAB94830; BAB94830; BAB94830.
GeneIDi31213838.
KEGGisam:MW0965.

Similar proteinsi

Entry informationi

Entry nameiPTHP_STAAW
AccessioniPrimary (citable) accession number: P0A0E2
Secondary accession number(s): P02907
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: March 1, 2005
Last modified: June 7, 2017
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families