Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mannitol-specific phosphotransferase enzyme IIA component

Gene

mtlF

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II CmtAB PTS system is involved in D-mannitol transport.By similarity

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-mannitol(Side 1) = [protein]-L-histidine + D-mannitol 1-phosphate(Side 2).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei63Tele-phosphohistidine intermediatePROSITE-ProRule annotationBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Mannitol-specific phosphotransferase enzyme IIA componentBy similarity (EC:2.7.1.197By similarity)
Alternative name(s):
EIIABy similarity
EIIIBy similarity
PTS system mannitol-specific EIIA componentBy similarity
Gene namesi
Name:mtlF
Synonyms:mtlA
Ordered Locus Names:SA1962
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000751 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001866382 – 144Mannitol-specific phosphotransferase enzyme IIA componentAdd BLAST143

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei63Phosphohistidine; by HPrBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homodimer or homotrimer. Seems to be a monomer when not phosphorylated.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0A0D8.
SMRiP0A0D8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 142PTS EIIA type-2PROSITE-ProRule annotationAdd BLAST140

Domaini

The PTS EIIA type-2 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-2 domain.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 PTS EIIA type-2 domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000227563.
KOiK02798.
OMAiISIVQVP.

Family and domain databases

Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
SUPFAMiSSF55804. SSF55804. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A0D8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELFSNDNI FLNVNVNSQN EAIEKAGKAL VDSGAVTDAY IQAMKDREQV
60 70 80 90 100
VSTFMGNGLA IPHGTDEAKT NVIHSGLTLL QIPEGVDWDG EVVKVVVGIA
110 120 130 140
GKDGEHLDLL SKIAITFSEE ENVDRIVQAK SAEEIKQVFE EADA
Length:144
Mass (Da):15,542
Last modified:January 23, 2007 - v2
Checksum:iB166851EEBC3A5AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB43251.1.
PIRiB90011.
RefSeqiWP_001292149.1. NC_002745.2.

Genome annotation databases

EnsemblBacteriaiBAB43251; BAB43251; BAB43251.
GeneIDi28380255.
KEGGisau:SA1962.
PATRICi19576390. VBIStaAur116463_2122.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB43251.1.
PIRiB90011.
RefSeqiWP_001292149.1. NC_002745.2.

3D structure databases

ProteinModelPortaliP0A0D8.
SMRiP0A0D8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB43251; BAB43251; BAB43251.
GeneIDi28380255.
KEGGisau:SA1962.
PATRICi19576390. VBIStaAur116463_2122.

Phylogenomic databases

HOGENOMiHOG000227563.
KOiK02798.
OMAiISIVQVP.

Family and domain databases

Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
SUPFAMiSSF55804. SSF55804. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTMA_STAAN
AccessioniPrimary (citable) accession number: P0A0D8
Secondary accession number(s): P17875, Q9RL67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.