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P0A0A5

- FEMA_STAAU

UniProt

P0A0A5 - FEMA_STAAU

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Protein

Aminoacyltransferase FemA

Gene

femA

Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the pentaglycine interpeptide bridge, which is characteristic of the S.aureus peptidoglycan. Adds glycines 2 and 3 of the pentaglycine bridge, using glycyl-tRNA(Gly) as donor.

Catalytic activityi

MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N(6)-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 glycyl-tRNA = MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N(6)-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 tRNA.

GO - Molecular functioni

  1. nucleotide binding Source: InterPro
  2. transferase activity, transferring amino-acyl groups Source: InterPro

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. peptidoglycan biosynthetic process Source: UniProtKB-KW
  3. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15453.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoacyltransferase FemA (EC:2.3.2.17)
Alternative name(s):
Factor essential for expression of methicillin resistance A
N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase
Gene namesi
Name:femA
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 420420Aminoacyltransferase FemAPRO_0000204735Add
BLAST

Proteomic databases

PRIDEiP0A0A5.

Interactioni

Subunit structurei

Homodimer. Interacts with FemB (By similarity).By similarity

Structurei

Secondary structure

1
420
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 54Combined sources
Helixi8 – 169Combined sources
Helixi27 – 359Combined sources
Beta strandi39 – 468Combined sources
Beta strandi52 – 6312Combined sources
Turni64 – 663Combined sources
Beta strandi67 – 715Combined sources
Helixi83 – 9816Combined sources
Turni99 – 1013Combined sources
Beta strandi102 – 1076Combined sources
Beta strandi112 – 1165Combined sources
Beta strandi122 – 1254Combined sources
Helixi130 – 1378Combined sources
Beta strandi151 – 1533Combined sources
Beta strandi156 – 1627Combined sources
Helixi168 – 1736Combined sources
Helixi177 – 18711Combined sources
Beta strandi192 – 1954Combined sources
Helixi198 – 2003Combined sources
Helixi201 – 2077Combined sources
Helixi222 – 23211Combined sources
Helixi233 – 2353Combined sources
Beta strandi239 – 2446Combined sources
Helixi245 – 27228Combined sources
Helixi277 – 30731Combined sources
Beta strandi309 – 32012Combined sources
Beta strandi325 – 3328Combined sources
Helixi334 – 3396Combined sources
Helixi341 – 35515Combined sources
Beta strandi360 – 3656Combined sources
Helixi377 – 3848Combined sources
Turni385 – 3873Combined sources
Beta strandi389 – 3935Combined sources
Beta strandi397 – 4026Combined sources
Helixi403 – 4119Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LRZX-ray2.10A1-420[»]
ProteinModelPortaliP0A0A5.
SMRiP0A0A5. Positions 1-412.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A0A5.

Family & Domainsi

Sequence similaritiesi

Belongs to the FemABX family.Curated

Phylogenomic databases

eggNOGiCOG2348.

Family and domain databases

Gene3Di3.40.630.30. 3 hits.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR003447. FEMABX.
IPR010978. tRNA-bd_arm.
[Graphical view]
PfamiPF02388. FemAB. 1 hit.
[Graphical view]
SUPFAMiSSF46589. SSF46589. 1 hit.
SSF55729. SSF55729. 3 hits.
PROSITEiPS51191. FEMABX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A0A5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFTNLTAKE FGAFTDSMPY SHFTQTVGHY ELKLAEGYET HLVGIKNNNN
60 70 80 90 100
EVIAACLLTA VPVMKVFKYF YSNRGPVIDY ENQELVHFFF NELSKYVKKH
110 120 130 140 150
RCLYLHIDPY LPYQYLNHDG EITGNAGNDW FFDKMSNLGF EHTGFHKGFD
160 170 180 190 200
PVLQIRYHSV LDLKDKTADD IIKNMDGLRK RNTKKVKKNG VKVRFLSEEE
210 220 230 240 250
LPIFRSFMED TSESKAFADR DDKFYYNRLK YYKDRVLVPL AYINFDEYIK
260 270 280 290 300
ELNEERDILN KDLNKALKDI EKRPENKKAH NKRDNLQQQL DANEQKIEEG
310 320 330 340 350
KRLQEEHGNE LPISAGFFFI NPFEVVYYAG GTSNAFRHFA GSYAVQWEMI
360 370 380 390 400
NYALNHGIDR YNFYGVSGKF TEDAEDAGVV KFKKGYNAEI IEYVGDFIKP
410 420
INKPVYAAYT ALKKVKDRIF
Length:420
Mass (Da):49,124
Last modified:April 18, 2006 - v2
Checksum:iE6790BD7C8B50297
GO

Sequence cautioni

The sequence CAA35679.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17688 Genomic DNA. Translation: CAA35679.1. Different initiation.
PIRiS06783.
RefSeqiWP_000673309.1. NZ_JFFV01000002.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17688 Genomic DNA. Translation: CAA35679.1 . Different initiation.
PIRi S06783.
RefSeqi WP_000673309.1. NZ_JFFV01000002.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1LRZ X-ray 2.10 A 1-420 [» ]
ProteinModelPortali P0A0A5.
SMRi P0A0A5. Positions 1-412.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P0A0A5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG2348.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-15453.

Miscellaneous databases

EvolutionaryTracei P0A0A5.

Family and domain databases

Gene3Di 3.40.630.30. 3 hits.
InterProi IPR016181. Acyl_CoA_acyltransferase.
IPR003447. FEMABX.
IPR010978. tRNA-bd_arm.
[Graphical view ]
Pfami PF02388. FemAB. 1 hit.
[Graphical view ]
SUPFAMi SSF46589. SSF46589. 1 hit.
SSF55729. SSF55729. 3 hits.
PROSITEi PS51191. FEMABX. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "FemA, a host-mediated factor essential for methicillin resistance in Staphylococcus aureus: molecular cloning and characterization."
    Berger-Baechi B., Barberis-Maino L., Straessle A., Kayser F.H.
    Mol. Gen. Genet. 219:263-269(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "FemA of Staphylococcus aureus: isolation and immunodetection."
    Johnson S., Krueger D., Labischinski H.
    FEMS Microbiol. Lett. 132:221-228(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: SG511.
  3. Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

Entry informationi

Entry nameiFEMA_STAAU
AccessioniPrimary (citable) accession number: P0A0A5
Secondary accession number(s): P14304
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: April 18, 2006
Last modified: November 26, 2014
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Since cross-linking between the peptide strands is critical for maintaining stability of the cell wall, FemA is a potential target for the development of new antibacterial agents.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3