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Protein

UDP-N-acetylmuramoylalanine--D-glutamate ligase

Gene

murD

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).By similarity

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanine + D-glutamate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate.

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi118 – 1247ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-1201-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC:6.3.2.9)
Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Gene namesi
Name:murD
Ordered Locus Names:SAV1183
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000002481 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449UDP-N-acetylmuramoylalanine--D-glutamate ligasePRO_0000109087Add
BLAST

Proteomic databases

PaxDbiP0A089.
PRIDEiP0A089.

2D gel databases

World-2DPAGE0002:P0A089.

Interactioni

Protein-protein interaction databases

STRINGi158878.SAV1183.

Structurei

3D structure databases

ProteinModelPortaliP0A089.
SMRiP0A089. Positions 5-444.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MurCDEF family.Curated

Phylogenomic databases

eggNOGiENOG4105DMZ. Bacteria.
COG0771. LUCA.
HOGENOMiHOG000049428.
KOiK01925.
OMAiPGIPYTN.
PhylomeDBiP0A089.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00639. MurD. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
PfamiPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01087. murD. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A089-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNYTGLENK NVLVVGLAKS GYEAAKLLSK LGANVTVNDG KDLSQDAHAK
60 70 80 90 100
DLESMGISVV SGSHPLTLLD NNPIIVKNPG IPYTVSIIDE AVKRGLKILT
110 120 130 140 150
EVELSYLISE APIIAVTGTN GKTTVTSLIG DMFKKSRLTG RLSGNIGYVA
160 170 180 190 200
SKVAQEVKPT DYLVTELSSF QLLGIEKYKP HIAIITNIYS AHLDYHENLE
210 220 230 240 250
NYQNAKKQIY KNQTEEDYLI CNYHQRQVIE SEELKAKTLY FSTQQEVDGI
260 270 280 290 300
YIKDGFIVYK GVRIINTEDL VLPGEHNLEN ILAAVLACIL AGVPIKAIID
310 320 330 340 350
SLTTFSGIEH RLQYVGTNRT NKYYNDSKAT NTLATQFALN SFNQPIIWLC
360 370 380 390 400
GGLDRGNEFD ELIPYMENVR AMVVFGQTKA KFAKLGNSQG KSVIEANNVE
410 420 430 440
DAVDKVQDII EPNDVVLLSP ACASWDQYST FEERGEKFIE RFRAHLPSY
Length:449
Mass (Da):49,844
Last modified:March 1, 2005 - v1
Checksum:i071BEEE9CF74F985
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB57345.1.
RefSeqiWP_000935991.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB57345; BAB57345; SAV1183.
KEGGisav:SAV1183.
PATRICi19563077. VBIStaAur52173_1216.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB57345.1.
RefSeqiWP_000935991.1. NC_002758.2.

3D structure databases

ProteinModelPortaliP0A089.
SMRiP0A089. Positions 5-444.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi158878.SAV1183.

2D gel databases

World-2DPAGE0002:P0A089.

Proteomic databases

PaxDbiP0A089.
PRIDEiP0A089.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB57345; BAB57345; SAV1183.
KEGGisav:SAV1183.
PATRICi19563077. VBIStaAur52173_1216.

Phylogenomic databases

eggNOGiENOG4105DMZ. Bacteria.
COG0771. LUCA.
HOGENOMiHOG000049428.
KOiK01925.
OMAiPGIPYTN.
PhylomeDBiP0A089.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciSAUR158878:GJJ5-1201-MONOMER.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00639. MurD. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
PfamiPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01087. murD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMURD_STAAM
AccessioniPrimary (citable) accession number: P0A089
Secondary accession number(s): O07323, O33595
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: September 7, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.