ID   MSRA2_STAA8             Reviewed;         177 AA.
AC   P0A086; Q2FYK8; Q93P63;
DT   15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   16-JUN-2009, entry version 22.
DE   RecName: Full=Peptide methionine sulfoxide reductase msrA 2;
DE            Short=Protein-methionine-S-oxide reductase 2;
DE            EC=1.8.4.11;
DE   AltName: Full=Peptide-methionine (S)-S-oxide reductase 2;
DE            Short=Peptide Met(O) reductase 2;
GN   Name=msrA2; Synonyms=msrA; OrderedLocusNames=SAOUHSC_01432;
OS   Staphylococcus aureus (strain NCTC 8325).
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CATALYTIC ACTIVITY.
RX   MEDLINE=21557163; PubMed=11700354;
RA   Singh V.K., Moskovitz J., Wilkinson B.J., Jayaswal R.K.;
RT   "Molecular characterization of a chromosomal locus in Staphylococcus
RT   aureus that contributes to oxidative defence and is highly induced by
RT   the cell-wall-active antibiotic oxacillin.";
RL   Microbiology 147:3037-3045(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W.,
RA   Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC8325 genome.";
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   CATALYTIC ACTIVITY, AND STEREOSPECIFICITY.
RX   MEDLINE=21637955; PubMed=11779133; DOI=10.1006/bbrc.2001.6171;
RA   Moskovitz J., Singh V.K., Requena J., Wilkinson B.J., Jayaswal R.K.,
RA   Stadtman E.R.;
RT   "Purification and characterization of methionine sulfoxide reductases
RT   from mouse and Staphylococcus aureus and their substrate
RT   stereospecificity.";
RL   Biochem. Biophys. Res. Commun. 290:62-65(2002).
CC   -!- FUNCTION: Has an important function as a repair enzyme for
CC       proteins that have been inactivated by oxidation. Catalyzes the
CC       reversible oxidation-reduction of methionine sulfoxide in proteins
CC       to methionine (By similarity).
CC   -!- CATALYTIC ACTIVITY: Peptide-L-methionine + thioredoxin disulfide +
CC       H(2)O = peptide-L-methionine (S)-S-oxide + thioredoxin.
CC   -!- CATALYTIC ACTIVITY: L-methionine + thioredoxin disulfide + H(2)O =
CC       L-methionine (S)-S-oxide + thioredoxin.
CC   -!- INDUCTION: Induced by oxacillin but not by hydrogen peroxide.
CC   -!- MISCELLANEOUS: Stereospecific for the S isomer of MetO.
CC   -!- SIMILARITY: Belongs to the msrA Met sulfoxide reductase family.
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DR   EMBL; AF349112; AAK83251.1; -; Genomic_DNA.
DR   EMBL; CP000253; ABD30524.1; -; Genomic_DNA.
DR   RefSeq; YP_499957.1; -.
DR   HSSP; P96814; 1NWA.
DR   GeneID; 3920213; -.
DR   GenomeReviews; CP000253_GR; SAOUHSC_01432.
DR   KEGG; sao:SAOUHSC_01432; -.
DR   HOGENOM; P0A086; -.
DR   OMA; P0A086; HFYKKNP.
DR   BioCyc; SAUR93061:SAOUHSC_01432-MON; -.
DR   GO; GO:0008113; F:peptide-methionine-(S)-S-oxide reductase ac...; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006464; P:protein modification process; IEA:HAMAP.
DR   HAMAP; MF_01401; -; 1.
DR   InterPro; IPR002569; MsrA.
DR   Gene3D; G3DSA:3.30.1060.10; MsrA; 1.
DR   Pfam; PF01625; PMSR; 1.
DR   ProDom; PD003489; PMSR; 1.
DR   TIGRFAMs; TIGR00401; msrA; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Oxidoreductase.
FT   CHAIN         1    177       Peptide methionine sulfoxide reductase
FT                                msrA 2.
FT                                /FTId=PRO_0000138590.
FT   ACT_SITE     12     12       By similarity.
SQ   SEQUENCE   177 AA;  20588 MW;  6BE5ABDE1545A5D4 CRC64;
     MTKEYATLAG GCFWCMVKPF TSYPGIKSVV SGYSGGHVDN PTYEQVCTNQ TGHVEAVQIT
     FDPEVTSFEN ILDIYFKTFD PTDDQGQFFD RGESYQPVIF YHDEHQKKAA EFKKQQLNEQ
     GIFKKPVITP IKPYKNFYPA EDYHQDYYKK NPVHYYQYQR GSGRKAFIES HWGNQNA
//