Reviewed, UniProtKB/Swiss-Prot P0A086 (MSRA2_STAA8)

Names and origin

Protein names

Recommended name:
    Peptide methionine sulfoxide reductase msrA 2
      Short name=Protein-methionine-S-oxide reductase 2
    EC=1.8.4.11
Alternative name(s):
    Peptide-methionine (S)-S-oxide reductase 2
      Short name=Peptide Met(O) reductase 2

Gene names

Name:	msrA2
Synonyms:	msrA
Ordered Locus Names:	SAOUHSC_01432

Organism

Staphylococcus aureus (strain NCTC 8325) [Complete proteome] [HAMAP]

Taxonomic identifier

93061 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine By similarity.
Catalytic activity	Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin. Ref.1 Ref.3 L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin. Ref.1 Ref.3
Induction	Induced by oxacillin but not by hydrogen peroxide. HAMAP MF_01401
Miscellaneous	Stereospecific for the S isomer of MetO. HAMAP MF_01401
Sequence similarities	Belongs to the msrA Met sulfoxide reductase family.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein modification process Inferred from electronic annotation. Source: HAMAP
Molecular function	peptide-methionine-(S)-S-oxide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	Peptide methionine sulfoxide reductase msrA 2 HAMAP MF_01401		PRO_0000138590
Sites
	Active site	12	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A086-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 6BE5ABDE1545A5D4	FASTA	177	20,588
	10 20 30 40 50 60 MTKEYATLAG GCFWCMVKPF TSYPGIKSVV SGYSGGHVDN PTYEQVCTNQ TGHVEAVQIT 70 80 90 100 110 120 FDPEVTSFEN ILDIYFKTFD PTDDQGQFFD RGESYQPVIF YHDEHQKKAA EFKKQQLNEQ 130 140 150 160 170 GIFKKPVITP IKPYKNFYPA EDYHQDYYKK NPVHYYQYQR GSGRKAFIES HWGNQNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of a chromosomal locus in Staphylococcus aureus that contributes to oxidative defence and is highly induced by the cell-wall-active antibiotic oxacillin." Singh V.K., Moskovitz J., Wilkinson B.J., Jayaswal R.K. Microbiology 147:3037-3045(2001) [PubMed: 11700354] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY.
[2]	"The Staphylococcus aureus NCTC8325 genome." Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Purification and characterization of methionine sulfoxide reductases from mouse and Staphylococcus aureus and their substrate stereospecificity." Moskovitz J., Singh V.K., Requena J., Wilkinson B.J., Jayaswal R.K., Stadtman E.R. Biochem. Biophys. Res. Commun. 290:62-65(2002) [PubMed: 11779133] [Abstract] Cited for: CATALYTIC ACTIVITY, STEREOSPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF349112 Genomic DNA. Translation: AAK83251.1. CP000253 Genomic DNA. Translation: ABD30524.1.
RefSeq	YP_499957.1.
3D structure databases
HSSP	HSSP built from PDB template 1NWA based on UniProtKB P96814.
ModBase	Search...
Genome annotation databases
GeneID	3920213.
GenomeReviews	Gene locus SAOUHSC_01432 in contig CP000253_GR.
KEGG	sao:SAOUHSC_01432.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A086.
OMA	P0A086. HFYKKNP.
Enzyme and pathway databases
BioCyc	SAUR93061:SAOUHSC_01432-MON.
Family and domain databases
HAMAP	MF_01401. [Tree]
InterPro	IPR002569. MsrA. [Graphical view]
Gene3D	G3DSA:3.30.1060.10. MsrA. 1 hit.
Pfam	PF01625. PMSR. 1 hit. [Graphical view]
ProDom	PD003489. PMSR. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00401. msrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MSRA2_STAA8

Accession

Primary (citable) accession number: P0A086
Secondary accession number(s): Q2FYK8, Q93P63

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	February 15, 2005
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)