Reviewed, UniProtKB/Swiss-Prot P0A070 (LEP_STAAU)

Names and origin

Protein names

Recommended name:
    Signal peptidase IB
      Short name=SPase IB
    EC=3.4.21.89
Alternative name(s):
    Leader peptidase IB

Gene names

Name:

spsB

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Essential for cell viability.
Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Hydrolase Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 191	191	Signal peptidase IB		PRO_0000109532
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 28	21	Potential
	Topological domain	29 – 191	163	Extracellular Potential
Sites
	Active site	36	1	By similarity
	Active site	77	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A070-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 1C6BF5BB423706C0	FASTA	191	21,692
	10 20 30 40 50 60 MKKELLEWII SIAVAFVILF IVGKFIVTPY TIKGESMDPT LKDGERVAVN IIGYKTGGLE 70 80 90 100 110 120 KGNVVVFHAN KNDDYVKRVI GVPGDKVEYK NDTLYVNGKK QDEPYLNYNL KHKQGDYITG 130 140 150 160 170 180 TFQVKDLPNA NPKSNVIPKG KYLVLGDNRE VSKDSRAFGL IDEDQIVGKV SFRFWPFSEF 190 KHNFNPENTK N

References

[1]

"Molecular cloning and expression of the spsB gene encoding an essential type I signal peptidase from Staphylococcus aureus." Cregg K.M., Wilding E.I., Black M.T. J. Bacteriol. 178:5712-5718(1996) [PubMed: 8824617] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WCUH29 / NCIMB 40771.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U65000 Genomic DNA. Translation: AAC44435.1.
3D structure databases
HSSP	HSSP built from PDB template 1B12 based on UniProtKB P00803.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.4.21.89. 95.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00501. SPASE_I_1. 1 hit. PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_STAAU

Accession

Primary (citable) accession number: P0A070
Secondary accession number(s): P72365

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)