P0A039 (GLNA_STAAW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine synthetase
Short name=GS
EC=6.3.1.2

Alternative name(s):
Glutamate--ammonia ligase

Gene names

Name:	glnA
Ordered Locus Names:	MW1192

Organism

Staphylococcus aureus (strain MW2) [Complete proteome] [HAMAP]

Taxonomic identifier

196620 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
Enzyme regulation	The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive By similarity.
Subunit structure	Oligomer of 12 subunits arranged in the form of two hexagons By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glutamine synthetase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamine biosynthetic process Inferred from electronic annotation. Source: InterPro nitrogen fixation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-ammonia ligase activity Inferred from electronic annotation. Source: UniProtKB-EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Glutamine synthetase		PRO_0000153263
Amino acid modifications
	Modified residue	375	1	O-AMP-tyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A039 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 81713EE654D7E7E6	FASTA	446	50,841
	10 20 30 40 50 60 MPKRTFTKDD IRKFAEEENV RYLRLQFTDI LGTIKNVEVP VSQLEKVLDN EMMFDGSSIE 70 80 90 100 110 120 GFVRIEESDM YLHPDLDTWV IFPWTAGQGK VARLICDVYK TDGTPFEGDP RANLKRVLKE 130 140 150 160 170 180 MEDLGFTDFN LGPEPEFFLF KLDEKGEPTL ELNDDGGYFD LAPTDLGENC RRDIVLELED 190 200 210 220 230 240 MGFDIEASHH EVAPGQHEID FKYADAVTAC DNIQTFKLVV KTIARKHNLH ATFMPKPLFG 250 260 270 280 290 300 VNGSGMHFNV SLFKGKENAF FDPNTEMGLT ETAYQFTAGV LKNARGFTAV CNPLVNSYKR 310 320 330 340 350 360 LVPGYEAPCY IAWSGKNRSP LIRVPSSRGL STRIEVRSVD PAANPYMALA AILEAGLDGI 370 380 390 400 410 420 KNKLKVPEPV NQNIYEMNRE EREAVGIQDL PSTLYTALKA MRENEVIKKA LGNHIYNQFI 430 440 NSKSIEWDYY RTQVSEWERD QYMKQY

References

[1]

"Genome and virulence determinants of high virulence community-acquired MRSA." Baba T., Takeuchi F., Kuroda M., Yuzawa H., Aoki K., Oguchi A., Nagai Y., Iwama N., Asano K., Naimi T., Kuroda H., Cui L., Yamamoto K., Hiramatsu K. Lancet 359:1819-1827(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MW2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000033 Genomic DNA. Translation: BAB95057.1.
RefSeq	NP_646009.1. NC_003923.1.
3D structure databases
ProteinModelPortal	P0A039.
SMR	P0A039. Positions 7-416.
ModBase	Search...
Protein-protein interaction databases
STRING	196620.MW1192.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB95057; BAB95057; BAB95057.
GeneID	1003304.
KEGG	sam:MW1192.
PATRIC	19568980. VBIStaAur44266_1251.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0174.
HOGENOM	HOG000005156.
KO	K01915.
OMA	AEDDVWS.
OrthoDB	EOG6B360N.
ProtClustDB	CLSK885265.
Enzyme and pathway databases
BioCyc	SAUR196620:GJ9Z-1216-MONOMER.
Family and domain databases
Gene3D	3.10.20.70. 1 hit. 3.30.590.10. 1 hit.
InterPro	IPR008147. Gln_synt_beta. IPR014746. Gln_synth/guanido_kin_cat_dom. IPR008146. Gln_synth_cat_dom. IPR027303. Gln_synth_gly_rich_site. IPR004809. Gln_synth_I. IPR027302. Gln_synth_N_conserv_site. [Graphical view]
Pfam	PF00120. Gln-synt_C. 1 hit. PF03951. Gln-synt_N. 1 hit. [Graphical view]
SUPFAM	SSF54368. SSF54368. 1 hit.
TIGRFAMs	TIGR00653. GlnA. 1 hit.
PROSITE	PS00180. GLNA_1. 1 hit. PS00181. GLNA_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNA_STAAW

Accession

Primary (citable) accession number: P0A039
Secondary accession number(s): Q59812

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	February 15, 2005
Last modified:	November 13, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program