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Protein

Beta sliding clamp

Gene

dnaN

Organism
Staphylococcus aureus (strain MW2)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
Beta sliding clamp
Short name:
Beta clamp
Short name:
Sliding clamp
Alternative name(s):
Beta-clamp processivity factor
DNA polymerase III beta sliding clamp subunit
DNA polymerase III subunit beta
Gene namesi
Name:dnaN
Ordered Locus Names:MW0002
OrganismiStaphylococcus aureus (strain MW2)
Taxonomic identifieri196620 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000418 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001054671 – 377Beta sliding clampAdd BLAST377

Interactioni

Subunit structurei

Forms a ring-shaped head-to-tail homodimer around DNA which binds and tethers DNA polymerases and other proteins to the DNA. The DNA replisome complex has a single clamp-loading complex (3 tau and 1 each of delta, delta', psi and chi subunits) which binds 3 Pol III cores (1 core on the leading strand and 2 on the lagging strand) each with a beta sliding clamp dimer. Additional proteins in the replisome are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA primase.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0A023.
SMRiP0A023.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta sliding clamp family.Curated

Phylogenomic databases

HOGENOMiHOG000071792.
KOiK02338.
OMAiNYEAVIP.

Family and domain databases

CDDicd00140. beta_clamp. 1 hit.
InterProiView protein in InterPro
IPR001001. DNA_polIII_beta.
IPR022635. DNA_polIII_beta_C.
IPR022637. DNA_polIII_beta_cen.
IPR022634. DNA_polIII_beta_N.
PANTHERiPTHR30478:SF1. PTHR30478:SF1. 1 hit.
PfamiView protein in Pfam
PF00712. DNA_pol3_beta. 1 hit.
PF02767. DNA_pol3_beta_2. 1 hit.
PF02768. DNA_pol3_beta_3. 1 hit.
PIRSFiPIRSF000804. DNA_pol_III_b. 1 hit.
SMARTiView protein in SMART
SM00480. POL3Bc. 1 hit.
TIGRFAMsiTIGR00663. dnan. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A023-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMEFTIKRDY FITQLNDTLK AISPRTTLPI LTGIKIDAKE HEVILTGSDS
60 70 80 90 100
EISIEITIPK TVDGEDIVNI SETGSVVLPG RFFVDIIKKL PGKDVKLSTN
110 120 130 140 150
EQFQTLITSG HSEFNLSGLD PDQYPLLPQV SRDDAIQLSV KVLKNVIAQT
160 170 180 190 200
NFAVSTSETR PVLTGVNWLI QENELICTAT DSHRLAVRKL QLEDVSENKN
210 220 230 240 250
VIIPGKALAE LNKIMSDNEE DIDIFFASNQ VLFKVGNVNF ISRLLEGHYP
260 270 280 290 300
DTTRLFPENY EIKLSIDNGE FYHAIDRASL LAREGGNNVI KLSTGDDVVE
310 320 330 340 350
LSSTSPEIGT VKEEVDANDV EGGSLKISFN SKYMMDALKA IDNDEVEVEF
360 370
FGTMKPFILK PKGDDSVTQL ILPIRTY
Length:377
Mass (Da):41,914
Last modified:March 1, 2005 - v1
Checksum:i0A985EF94E044FBC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA. Translation: BAB93867.1.
RefSeqiWP_000969811.1. NC_003923.1.

Genome annotation databases

EnsemblBacteriaiBAB93867; BAB93867; BAB93867.
GeneIDi31212826.
KEGGisam:MW0002.

Similar proteinsi

Entry informationi

Entry nameiDPO3B_STAAW
AccessioniPrimary (citable) accession number: P0A023
Secondary accession number(s): P50029
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: September 27, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families