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Reviewed, UniProtKB/Swiss-Prot P0A017 (DYR_STAAU)

Last modified June 16, 2009. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Dihydrofolate reductase
      Short name=DHFR
    EC=1.5.1.3
Gene names
Name: folA
OrganismStaphylococcus aureus
Taxonomic identifier1280 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacillalesStaphylococcus

Protein attributes

Sequence length159 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.

Miscellaneous

There are two dhfR isozymes in S.aureus, this one is chromosomal and is sensitive to trimethoprim.

The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2
Chain2 – 159158Dihydrofolate reductase
PRO_0000186411

Regions

Domain2 – 157156DHFR

Sequences

Sequence LengthMass (Da)Tools
P0A017-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 811898409FEAFAAB

FASTA15918,251
        10         20         30         40         50         60 
MTLSILVAHD LQRVIGFENQ LPWHLPNDLK HVKKLSTGHT LVMGRKTFES IGKPLPNRRN 

        70         80         90        100        110        120 
VVLTSDTSFN VEGVDVIHSI EDIYQLPGHV FIFGGQTLFE EMIDKVDDMY ITVIEGKFRG 

       130        140        150 
DTFFPPYTFE DWEVASSVEG KLDEKNTIPH TFLHLIRKK 

« Hide

References

[1]"Characterization of the gene for chromosomal trimethoprim-sensitive dihydrofolate reductase of Staphylococcus aureus ATCC 25923."
Dale G.E., Then R.L., Stueber D.
Antimicrob. Agents Chemother. 37:1400-1405(1993) [PubMed: 8363365] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 25923 / DSM 1104 / Seattle 1945 / FO 14462 / JCM 2413.
[2]"N-terminal amino acid sequence of the chromosomal dihydrofolate reductase purified from trimethoprim-resistant Staphylococcus aureus."
Hartman P.G., Stahli M., Kocher H.P., Then R.L.
FEBS Lett. 242:157-160(1988) [PubMed: 3060373] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-36.
Strain: 157/4696.

Cross-references

Sequence databases

Z16422 Genomic DNA. Translation: CAA78910.1.
PIRS32014.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2W9GX-ray1.95A1-159[»]
2W9HX-ray1.48A1-159[»]
ModBaseSearch...

Enzyme and pathway databases

BRENDA1.5.1.3. 95.

Family and domain databases

InterProIPR012259. DHFR.
IPR001796. DHFR_reg.
IPR017925. Dihydrofolate_reductase_CS.
[Graphical view]
PANTHERPTHR11549:SF1. DHFR. 1 hit.
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSPR00070. DHFR.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

BindingDBP0A017.

Entry information

Entry nameDYR_STAAU
AccessionPrimary (citable) accession number: P0A017
Secondary accession number(s): P10167
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 27 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents