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Protein

Ribosome-inactivating protein alpha-trichosanthin

Gene
N/A
Organism
Trichosanthes kirilowii (Chinese snake gourd) (Chinese cucumber)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inactivates eukaryotic 60S ribosomal subunits.

Catalytic activityi

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei183By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antiviral protein, Hydrolase, Protein synthesis inhibitor, Toxin

Keywords - Biological processi

Plant defense

Enzyme and pathway databases

BRENDAi3.2.2.22. 6463.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome-inactivating protein alpha-trichosanthin (EC:3.2.2.22)
Short name:
Alpha-TCS
Alternative name(s):
rRNA N-glycosidase
OrganismiTrichosanthes kirilowii (Chinese snake gourd) (Chinese cucumber)
Taxonomic identifieri3677 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeSicyoeaeTrichosanthes

Pathology & Biotechi

Protein family/group databases

Allergomei2807. Tri k RIP.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 232 PublicationsAdd BLAST23
ChainiPRO_000003076524 – 270Ribosome-inactivating protein alpha-trichosanthinAdd BLAST247
PropeptideiPRO_0000030766271 – 289Removed in mature formAdd BLAST19

Interactioni

Protein-protein interaction databases

MINTiMINT-193117.

Structurei

Secondary structure

1289
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi25 – 28Combined sources4
Helixi34 – 46Combined sources13
Beta strandi50 – 54Combined sources5
Beta strandi57 – 60Combined sources4
Helixi66 – 69Combined sources4
Beta strandi70 – 76Combined sources7
Beta strandi82 – 88Combined sources7
Turni89 – 91Combined sources3
Beta strandi94 – 99Combined sources6
Beta strandi102 – 105Combined sources4
Helixi109 – 114Combined sources6
Turni115 – 117Combined sources3
Beta strandi123 – 127Combined sources5
Helixi134 – 141Combined sources8
Helixi145 – 147Combined sources3
Helixi152 – 163Combined sources12
Helixi167 – 180Combined sources14
Helixi182 – 186Combined sources5
Helixi188 – 195Combined sources8
Beta strandi198 – 200Combined sources3
Helixi206 – 226Combined sources21
Turni227 – 230Combined sources4
Beta strandi231 – 239Combined sources9
Beta strandi245 – 250Combined sources6
Helixi254 – 258Combined sources5
Turni266 – 268Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GISX-ray1.70A24-270[»]
1GIUX-ray1.80A24-270[»]
1J4GX-ray2.00A/B/C/D24-270[»]
1MRJX-ray1.60A24-270[»]
1MRKX-ray1.60A24-270[»]
1NLIX-ray1.93A24-270[»]
1QD2X-ray1.86A24-270[»]
1TCSX-ray1.70A24-270[»]
2JDLX-ray2.20A/B25-270[»]
2JJRX-ray2.30A24-270[»]
2VS6X-ray2.40A/B24-270[»]
ProteinModelPortaliP09989.
SMRiP09989.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09989.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PRINTSiPR00396. SHIGARICIN.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRFLVLSLL ILTLFLTTPA VEGDVSFRLS GATSSSYGVF ISNLRKALPN
60 70 80 90 100
ERKLYDIPLL RSSLPGSQRY ALIHLTNYAD ETISVAIDVT NVYIMGYRAG
110 120 130 140 150
DTSYFFNEAS ATEAAKYVFK DAMRKVTLPY SGNYERLQTA AGKIRENIPL
160 170 180 190 200
GLPALDSAIT TLFYYNANSA ASALMVLIQS TSEAARYKFI EQQIGKRVDK
210 220 230 240 250
TFLPSLAIIS LENSWSALSK QIQIASTNNG QFESPVVLIN AQNQRVTITN
260 270 280
VDAGVVTSNI ALLLNRNNMA AMDDDVPMTQ SFGCGSYAI
Length:289
Mass (Da):31,676
Last modified:November 1, 1990 - v2
Checksum:i5CE09BB630575BB9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57 – 60IPLL → LPLI AA sequence (Ref. 4) Curated4
Sequence conflicti82 – 84Missing AA sequence (Ref. 4) Curated3
Sequence conflicti87I → L AA sequence (Ref. 4) Curated1
Sequence conflicti92V → VDAGLPRNAVL AA sequence (Ref. 4) Curated1
Sequence conflicti143 – 144KI → GL AA sequence (Ref. 4) Curated2
Sequence conflicti196K → S AA sequence (Ref. 4) Curated1
Sequence conflicti215 – 216WS → LWL AA sequence (Ref. 4) Curated2
Sequence conflicti231Q → T AA sequence (Ref. 4) Curated1
Sequence conflicti234S → T in AAA34206 (PubMed:2341400).Curated1
Sequence conflicti246 – 266Missing AA sequence (Ref. 4) CuratedAdd BLAST21
Sequence conflicti247T → M in AAA34206 (PubMed:2341400).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34858 mRNA. Translation: AAA34207.1.
J05434 Genomic DNA. Translation: AAA34206.1.
PIRiJT0566. RLTZT.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34858 mRNA. Translation: AAA34207.1.
J05434 Genomic DNA. Translation: AAA34206.1.
PIRiJT0566. RLTZT.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GISX-ray1.70A24-270[»]
1GIUX-ray1.80A24-270[»]
1J4GX-ray2.00A/B/C/D24-270[»]
1MRJX-ray1.60A24-270[»]
1MRKX-ray1.60A24-270[»]
1NLIX-ray1.93A24-270[»]
1QD2X-ray1.86A24-270[»]
1TCSX-ray1.70A24-270[»]
2JDLX-ray2.20A/B25-270[»]
2JJRX-ray2.30A24-270[»]
2VS6X-ray2.40A/B24-270[»]
ProteinModelPortaliP09989.
SMRiP09989.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-193117.

Protein family/group databases

Allergomei2807. Tri k RIP.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.2.2.22. 6463.

Miscellaneous databases

EvolutionaryTraceiP09989.

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PRINTSiPR00396. SHIGARICIN.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIPT_TRIKI
AccessioniPrimary (citable) accession number: P09989
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1990
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Abortion-inducing protein. Inhibits HIV-1 infection and replication.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.