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P09989 (RIPT_TRIKI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosome-inactivating protein alpha-trichosanthin

Short name=Alpha-TCS
EC=3.2.2.22
Alternative name(s):
rRNA N-glycosidase
OrganismTrichosanthes kirilowii (Chinese snake gourd) (Chinese cucumber)
Taxonomic identifier3677 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeSicyoeaeTrichosanthes

Protein attributes

Sequence length289 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inactivates eukaryotic 60S ribosomal subunits.

Catalytic activity

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

Miscellaneous

Abortion-inducing protein. Inhibits HIV-1 infection and replication.

Sequence similarities

Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Ref.3 Ref.4
Chain24 – 270247Ribosome-inactivating protein alpha-trichosanthin
PRO_0000030765
Propeptide271 – 28919Removed in mature form
PRO_0000030766

Sites

Active site1831 By similarity

Experimental info

Sequence conflict57 – 604IPLL → LPLI AA sequence Ref.4
Sequence conflict82 – 843Missing AA sequence Ref.4
Sequence conflict871I → L AA sequence Ref.4
Sequence conflict921V → VDAGLPRNAVL AA sequence Ref.4
Sequence conflict143 – 1442KI → GL AA sequence Ref.4
Sequence conflict1961K → S AA sequence Ref.4
Sequence conflict215 – 2162WS → LWL AA sequence Ref.4
Sequence conflict2311Q → T AA sequence Ref.4
Sequence conflict2341S → T in AAA34206. Ref.2
Sequence conflict246 – 26621Missing AA sequence Ref.4
Sequence conflict2471T → M in AAA34206. Ref.2

Secondary structure

................................................ 289
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P09989 [UniParc].

Last modified November 1, 1990. Version 2.
Checksum: 5CE09BB630575BB9

FASTA28931,676
        10         20         30         40         50         60 
MIRFLVLSLL ILTLFLTTPA VEGDVSFRLS GATSSSYGVF ISNLRKALPN ERKLYDIPLL 

        70         80         90        100        110        120 
RSSLPGSQRY ALIHLTNYAD ETISVAIDVT NVYIMGYRAG DTSYFFNEAS ATEAAKYVFK 

       130        140        150        160        170        180 
DAMRKVTLPY SGNYERLQTA AGKIRENIPL GLPALDSAIT TLFYYNANSA ASALMVLIQS 

       190        200        210        220        230        240 
TSEAARYKFI EQQIGKRVDK TFLPSLAIIS LENSWSALSK QIQIASTNNG QFESPVVLIN 

       250        260        270        280 
AQNQRVTITN VDAGVVTSNI ALLLNRNNMA AMDDDVPMTQ SFGCGSYAI 

« Hide

References

[1]"Cloning of trichosanthin cDNA and its expression in Escherichia coli."
Shaw P.C., Yung M.H., Zhu R.H., Ho W.K.K., Ng T.B., Yeung H.W.
Gene 97:267-272(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Maximowicz.
[2]"Isolation and DNA sequence of a gene encoding alpha-trichosanthin, a type I ribosome-inactivating protein."
Chow T., Feldman R.A., Lovett M., Piatak M.
J. Biol. Chem. 265:8670-8674(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Maximowicz.
Tissue: Leaf.
[3]"Primary amino acid sequence of alpha-trichosanthin and molecular models for abrin A-chain and alpha-trichosanthin."
Collins E.J., Robertus J.D., Lopresti M., Stone K.L., Williams K.R., Wu P., Hwang K., Piatak M.
J. Biol. Chem. 265:8665-8669(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 24-270.
Strain: Maximowicz.
Tissue: Tuberous root.
[4]"Scientific evaluation of Tian Hua Fen (THF): history, chemistry and application."
Wang Y., Qian R.Q., Gu Z.W., Jin S.W., Zhang L.Q., Xia Z.X., Tian G.Y., Ni C.Z.
Pure Appl. Chem. 58:789-798(1986)
Cited for: PROTEIN SEQUENCE OF 24-270.
Tissue: Tuberous root.
[5]"Structure of trichosanthin at 1.88-A resolution."
Zhou F., Fu Z., Chen M., Lin Y., Pan K.
Proteins 19:4-13(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.88 ANGSTROMS).
[6]"Studies on crystal structures, active-centre geometry and depurinating mechanism of two ribosome-inactivating proteins."
Huang Q., Liu S., Tang Y., Jin S., Wang Y.
Biochem. J. 309:285-298(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M34858 mRNA. Translation: AAA34207.1.
J05434 Genomic DNA. Translation: AAA34206.1.
PIRRLTZT. JT0566.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GISX-ray1.70A24-270[»]
1GIUX-ray1.80A24-270[»]
1J4GX-ray2.00A/B/C/D24-270[»]
1MRJX-ray1.60A24-270[»]
1MRKX-ray1.60A24-270[»]
1NLIX-ray1.93A24-270[»]
1QD2X-ray1.86A24-270[»]
1TCSX-ray1.70A24-270[»]
2JDLX-ray2.20A/B25-270[»]
2JJRX-ray2.30A24-270[»]
2VS6X-ray2.40A/B24-270[»]
ProteinModelPortalP09989.
SMRP09989. Positions 24-270.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-193117.

Chemistry

DrugBankDB00173. Adenine.
DB00131. Adenosine monophosphate.

Protein family/group databases

Allergome2807. Tri k RIP.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]
PfamPF00161. RIP. 1 hit.
[Graphical view]
PRINTSPR00396. SHIGARICIN.
SUPFAMSSF56371. SSF56371. 1 hit.
PROSITEPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP09989.

Entry information

Entry nameRIPT_TRIKI
AccessionPrimary (citable) accession number: P09989
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1990
Last modified: April 16, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references