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Protein

Ribosome-inactivating protein alpha-trichosanthin

Gene
N/A
Organism
Trichosanthes kirilowii (Chinese snake gourd) (Chinese cucumber)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inactivates eukaryotic 60S ribosomal subunits.

Miscellaneous

Abortion-inducing protein. Inhibits HIV-1 infection and replication.

Catalytic activityi

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei183By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntiviral protein, Hydrolase, Protein synthesis inhibitor, Toxin
Biological processPlant defense

Enzyme and pathway databases

BRENDAi3.2.2.22. 6463.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome-inactivating protein alpha-trichosanthin (EC:3.2.2.22)
Short name:
Alpha-TCS
Alternative name(s):
rRNA N-glycosidase
OrganismiTrichosanthes kirilowii (Chinese snake gourd) (Chinese cucumber)
Taxonomic identifieri3677 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeSicyoeaeTrichosanthes

Pathology & Biotechi

Protein family/group databases

Allergomei2807. Tri k RIP.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 232 PublicationsAdd BLAST23
ChainiPRO_000003076524 – 270Ribosome-inactivating protein alpha-trichosanthinAdd BLAST247
PropeptideiPRO_0000030766271 – 289Removed in mature formAdd BLAST19

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

IntActiP09989. 3 interactors.
MINTiMINT-193117.

Structurei

Secondary structure

1289
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi25 – 28Combined sources4
Helixi34 – 46Combined sources13
Beta strandi50 – 54Combined sources5
Beta strandi57 – 60Combined sources4
Helixi66 – 69Combined sources4
Beta strandi70 – 76Combined sources7
Beta strandi82 – 88Combined sources7
Turni89 – 91Combined sources3
Beta strandi94 – 99Combined sources6
Beta strandi102 – 105Combined sources4
Helixi109 – 114Combined sources6
Turni115 – 117Combined sources3
Beta strandi123 – 127Combined sources5
Helixi134 – 141Combined sources8
Helixi145 – 147Combined sources3
Helixi152 – 163Combined sources12
Helixi167 – 180Combined sources14
Helixi182 – 186Combined sources5
Helixi188 – 195Combined sources8
Beta strandi198 – 200Combined sources3
Helixi206 – 226Combined sources21
Turni227 – 230Combined sources4
Beta strandi231 – 239Combined sources9
Beta strandi245 – 250Combined sources6
Helixi254 – 258Combined sources5
Turni266 – 268Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GISX-ray1.70A24-270[»]
1GIUX-ray1.80A24-270[»]
1J4GX-ray2.00A/B/C/D24-270[»]
1MRJX-ray1.60A24-270[»]
1MRKX-ray1.60A24-270[»]
1NLIX-ray1.93A24-270[»]
1QD2X-ray1.86A24-270[»]
1TCSX-ray1.70A24-270[»]
2JDLX-ray2.20A/B25-270[»]
2JJRX-ray2.30A24-270[»]
2VS6X-ray2.40A/B24-270[»]
ProteinModelPortaliP09989.
SMRiP09989.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09989.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiView protein in InterPro
IPR017989. Ribosome_inactivat_1/2.
IPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
PfamiView protein in Pfam
PF00161. RIP. 1 hit.
PRINTSiPR00396. SHIGARICIN.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiView protein in PROSITE
PS00275. SHIGA_RICIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRFLVLSLL ILTLFLTTPA VEGDVSFRLS GATSSSYGVF ISNLRKALPN
60 70 80 90 100
ERKLYDIPLL RSSLPGSQRY ALIHLTNYAD ETISVAIDVT NVYIMGYRAG
110 120 130 140 150
DTSYFFNEAS ATEAAKYVFK DAMRKVTLPY SGNYERLQTA AGKIRENIPL
160 170 180 190 200
GLPALDSAIT TLFYYNANSA ASALMVLIQS TSEAARYKFI EQQIGKRVDK
210 220 230 240 250
TFLPSLAIIS LENSWSALSK QIQIASTNNG QFESPVVLIN AQNQRVTITN
260 270 280
VDAGVVTSNI ALLLNRNNMA AMDDDVPMTQ SFGCGSYAI
Length:289
Mass (Da):31,676
Last modified:November 1, 1990 - v2
Checksum:i5CE09BB630575BB9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57 – 60IPLL → LPLI AA sequence (Ref. 4) Curated4
Sequence conflicti82 – 84Missing AA sequence (Ref. 4) Curated3
Sequence conflicti87I → L AA sequence (Ref. 4) Curated1
Sequence conflicti92V → VDAGLPRNAVL AA sequence (Ref. 4) Curated1
Sequence conflicti143 – 144KI → GL AA sequence (Ref. 4) Curated2
Sequence conflicti196K → S AA sequence (Ref. 4) Curated1
Sequence conflicti215 – 216WS → LWL AA sequence (Ref. 4) Curated2
Sequence conflicti231Q → T AA sequence (Ref. 4) Curated1
Sequence conflicti234S → T in AAA34206 (PubMed:2341400).Curated1
Sequence conflicti246 – 266Missing AA sequence (Ref. 4) CuratedAdd BLAST21
Sequence conflicti247T → M in AAA34206 (PubMed:2341400).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34858 mRNA. Translation: AAA34207.1.
J05434 Genomic DNA. Translation: AAA34206.1.
PIRiJT0566. RLTZT.

Similar proteinsi

Entry informationi

Entry nameiRIPT_TRIKI
AccessioniPrimary (citable) accession number: P09989
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1990
Last modified: September 27, 2017
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families