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P09989

- RIPT_TRIKI

UniProt

P09989 - RIPT_TRIKI

Protein

Ribosome-inactivating protein alpha-trichosanthin

Gene
N/A
Organism
Trichosanthes kirilowii (Chinese snake gourd) (Chinese cucumber)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 2 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    Inactivates eukaryotic 60S ribosomal subunits.

    Catalytic activityi

    Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei183 – 1831By similarity

    GO - Molecular functioni

    1. rRNA N-glycosylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. defense response Source: UniProtKB-KW
    2. negative regulation of translation Source: UniProtKB-KW
    3. regulation of defense response to virus Source: UniProtKB-KW

    Keywords - Molecular functioni

    Antiviral protein, Hydrolase, Protein synthesis inhibitor, Toxin

    Keywords - Biological processi

    Plant defense

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribosome-inactivating protein alpha-trichosanthin (EC:3.2.2.22)
    Short name:
    Alpha-TCS
    Alternative name(s):
    rRNA N-glycosidase
    OrganismiTrichosanthes kirilowii (Chinese snake gourd) (Chinese cucumber)
    Taxonomic identifieri3677 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeSicyoeaeTrichosanthes

    Pathology & Biotechi

    Protein family/group databases

    Allergomei2807. Tri k RIP.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 23232 PublicationsAdd
    BLAST
    Chaini24 – 270247Ribosome-inactivating protein alpha-trichosanthinPRO_0000030765Add
    BLAST
    Propeptidei271 – 28919Removed in mature formPRO_0000030766Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-193117.

    Structurei

    Secondary structure

    1
    289
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi25 – 284
    Helixi34 – 4613
    Beta strandi50 – 545
    Beta strandi57 – 604
    Helixi66 – 694
    Beta strandi70 – 767
    Beta strandi82 – 887
    Turni89 – 913
    Beta strandi94 – 996
    Beta strandi102 – 1054
    Helixi109 – 1146
    Turni115 – 1173
    Beta strandi123 – 1275
    Helixi134 – 1418
    Helixi145 – 1473
    Helixi152 – 16312
    Helixi167 – 18014
    Helixi182 – 1865
    Helixi188 – 1958
    Beta strandi198 – 2003
    Helixi206 – 22621
    Turni227 – 2304
    Beta strandi231 – 2399
    Beta strandi245 – 2506
    Helixi254 – 2585
    Turni266 – 2683

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GISX-ray1.70A24-270[»]
    1GIUX-ray1.80A24-270[»]
    1J4GX-ray2.00A/B/C/D24-270[»]
    1MRJX-ray1.60A24-270[»]
    1MRKX-ray1.60A24-270[»]
    1NLIX-ray1.93A24-270[»]
    1QD2X-ray1.86A24-270[»]
    1TCSX-ray1.70A24-270[»]
    2JDLX-ray2.20A/B25-270[»]
    2JJRX-ray2.30A24-270[»]
    2VS6X-ray2.40A/B24-270[»]
    ProteinModelPortaliP09989.
    SMRiP09989. Positions 24-270.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP09989.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.420.10. 1 hit.
    4.10.470.10. 1 hit.
    InterProiIPR001574. Ribosome_inactivat_prot.
    IPR017988. Ribosome_inactivat_prot_CS.
    IPR016138. Ribosome_inactivat_prot_sub1.
    IPR016139. Ribosome_inactivat_prot_sub2.
    IPR017989. Ribosome_inactivat_prot_subgr.
    [Graphical view]
    PfamiPF00161. RIP. 1 hit.
    [Graphical view]
    PRINTSiPR00396. SHIGARICIN.
    SUPFAMiSSF56371. SSF56371. 1 hit.
    PROSITEiPS00275. SHIGA_RICIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09989-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIRFLVLSLL ILTLFLTTPA VEGDVSFRLS GATSSSYGVF ISNLRKALPN    50
    ERKLYDIPLL RSSLPGSQRY ALIHLTNYAD ETISVAIDVT NVYIMGYRAG 100
    DTSYFFNEAS ATEAAKYVFK DAMRKVTLPY SGNYERLQTA AGKIRENIPL 150
    GLPALDSAIT TLFYYNANSA ASALMVLIQS TSEAARYKFI EQQIGKRVDK 200
    TFLPSLAIIS LENSWSALSK QIQIASTNNG QFESPVVLIN AQNQRVTITN 250
    VDAGVVTSNI ALLLNRNNMA AMDDDVPMTQ SFGCGSYAI 289
    Length:289
    Mass (Da):31,676
    Last modified:November 1, 1990 - v2
    Checksum:i5CE09BB630575BB9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti57 – 604IPLL → LPLI AA sequence 1 PublicationCurated
    Sequence conflicti82 – 843Missing AA sequence 1 PublicationCurated
    Sequence conflicti87 – 871I → L AA sequence 1 PublicationCurated
    Sequence conflicti92 – 921V → VDAGLPRNAVL AA sequence 1 PublicationCurated
    Sequence conflicti143 – 1442KI → GL AA sequence 1 PublicationCurated
    Sequence conflicti196 – 1961K → S AA sequence 1 PublicationCurated
    Sequence conflicti215 – 2162WS → LWL AA sequence 1 PublicationCurated
    Sequence conflicti231 – 2311Q → T AA sequence 1 PublicationCurated
    Sequence conflicti234 – 2341S → T in AAA34206. (PubMed:2341400)Curated
    Sequence conflicti246 – 26621Missing AA sequence 1 PublicationCuratedAdd
    BLAST
    Sequence conflicti247 – 2471T → M in AAA34206. (PubMed:2341400)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34858 mRNA. Translation: AAA34207.1.
    J05434 Genomic DNA. Translation: AAA34206.1.
    PIRiJT0566. RLTZT.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34858 mRNA. Translation: AAA34207.1 .
    J05434 Genomic DNA. Translation: AAA34206.1 .
    PIRi JT0566. RLTZT.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GIS X-ray 1.70 A 24-270 [» ]
    1GIU X-ray 1.80 A 24-270 [» ]
    1J4G X-ray 2.00 A/B/C/D 24-270 [» ]
    1MRJ X-ray 1.60 A 24-270 [» ]
    1MRK X-ray 1.60 A 24-270 [» ]
    1NLI X-ray 1.93 A 24-270 [» ]
    1QD2 X-ray 1.86 A 24-270 [» ]
    1TCS X-ray 1.70 A 24-270 [» ]
    2JDL X-ray 2.20 A/B 25-270 [» ]
    2JJR X-ray 2.30 A 24-270 [» ]
    2VS6 X-ray 2.40 A/B 24-270 [» ]
    ProteinModelPortali P09989.
    SMRi P09989. Positions 24-270.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-193117.

    Chemistry

    DrugBanki DB00173. Adenine.
    DB00131. Adenosine monophosphate.

    Protein family/group databases

    Allergomei 2807. Tri k RIP.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P09989.

    Family and domain databases

    Gene3Di 3.40.420.10. 1 hit.
    4.10.470.10. 1 hit.
    InterProi IPR001574. Ribosome_inactivat_prot.
    IPR017988. Ribosome_inactivat_prot_CS.
    IPR016138. Ribosome_inactivat_prot_sub1.
    IPR016139. Ribosome_inactivat_prot_sub2.
    IPR017989. Ribosome_inactivat_prot_subgr.
    [Graphical view ]
    Pfami PF00161. RIP. 1 hit.
    [Graphical view ]
    PRINTSi PR00396. SHIGARICIN.
    SUPFAMi SSF56371. SSF56371. 1 hit.
    PROSITEi PS00275. SHIGA_RICIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of trichosanthin cDNA and its expression in Escherichia coli."
      Shaw P.C., Yung M.H., Zhu R.H., Ho W.K.K., Ng T.B., Yeung H.W.
      Gene 97:267-272(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Maximowicz.
    2. "Isolation and DNA sequence of a gene encoding alpha-trichosanthin, a type I ribosome-inactivating protein."
      Chow T., Feldman R.A., Lovett M., Piatak M.
      J. Biol. Chem. 265:8670-8674(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Maximowicz.
      Tissue: Leaf.
    3. "Primary amino acid sequence of alpha-trichosanthin and molecular models for abrin A-chain and alpha-trichosanthin."
      Collins E.J., Robertus J.D., Lopresti M., Stone K.L., Williams K.R., Wu P., Hwang K., Piatak M.
      J. Biol. Chem. 265:8665-8669(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-270.
      Strain: Maximowicz.
      Tissue: Tuberous root.
    4. "Scientific evaluation of Tian Hua Fen (THF): history, chemistry and application."
      Wang Y., Qian R.Q., Gu Z.W., Jin S.W., Zhang L.Q., Xia Z.X., Tian G.Y., Ni C.Z.
      Pure Appl. Chem. 58:789-798(1986)
      Cited for: PROTEIN SEQUENCE OF 24-270.
      Tissue: Tuberous root.
    5. "Structure of trichosanthin at 1.88-A resolution."
      Zhou F., Fu Z., Chen M., Lin Y., Pan K.
      Proteins 19:4-13(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.88 ANGSTROMS).
    6. "Studies on crystal structures, active-centre geometry and depurinating mechanism of two ribosome-inactivating proteins."
      Huang Q., Liu S., Tang Y., Jin S., Wang Y.
      Biochem. J. 309:285-298(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

    Entry informationi

    Entry nameiRIPT_TRIKI
    AccessioniPrimary (citable) accession number: P09989
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 100 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Abortion-inducing protein. Inhibits HIV-1 infection and replication.

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3