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Protein

ATP-dependent DNA helicase Rep

Gene

rep

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction.UniRule annotation1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation2 Publications

Enzyme regulationi

Binding to DNA induces dimerization, which is required for DNA helicase activity.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei278ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 29ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

  • bacterial-type DNA replication Source: EcoCyc
  • DNA unwinding involved in DNA replication Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10837-MONOMER.
ECOL316407:JW5604-MONOMER.
MetaCyc:EG10837-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase RepUniRule annotation (EC:3.6.4.12UniRule annotation)
Gene namesi
Name:repUniRule annotation
Ordered Locus Names:b3778, JW5604
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10837. rep.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001020681 – 673ATP-dependent DNA helicase RepAdd BLAST673

Proteomic databases

PaxDbiP09980.
PRIDEiP09980.

Interactioni

Subunit structurei

Homodimer.UniRule annotation2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
dnaBP0ACB03EBI-6558011,EBI-548978

Protein-protein interaction databases

BioGridi4263317. 66 interactors.
DIPiDIP-10662N.
IntActiP09980. 4 interactors.
MINTiMINT-1286362.
STRINGi511145.b3778.

Structurei

Secondary structure

1673
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 12Combined sources8
Beta strandi15 – 20Combined sources6
Helixi28 – 43Combined sources16
Helixi47 – 49Combined sources3
Beta strandi50 – 56Combined sources7
Helixi57 – 70Combined sources14
Turni73 – 78Combined sources6
Beta strandi79 – 83Combined sources5
Helixi84 – 98Combined sources15
Helixi110 – 120Combined sources11
Helixi129 – 143Combined sources15
Turni144 – 146Combined sources3
Helixi151 – 154Combined sources4
Helixi159 – 178Combined sources20
Helixi183 – 196Combined sources14
Helixi198 – 205Combined sources8
Beta strandi209 – 214Combined sources6
Helixi216 – 218Combined sources3
Helixi221 – 231Combined sources11
Turni232 – 234Combined sources3
Beta strandi237 – 240Combined sources4
Helixi243 – 245Combined sources3
Helixi249 – 251Combined sources3
Helixi257 – 264Combined sources8
Beta strandi269 – 271Combined sources3
Beta strandi276 – 279Combined sources4
Helixi281 – 292Combined sources12
Beta strandi304 – 306Combined sources3
Beta strandi312 – 316Combined sources5
Helixi320 – 338Combined sources19
Turni342 – 344Combined sources3
Beta strandi345 – 352Combined sources8
Helixi353 – 355Combined sources3
Helixi358 – 364Combined sources7
Beta strandi369 – 372Combined sources4
Helixi377 – 379Combined sources3
Helixi381 – 394Combined sources14
Helixi399 – 405Combined sources7
Helixi415 – 427Combined sources13
Helixi432 – 435Combined sources4
Helixi441 – 444Combined sources4
Helixi448 – 467Combined sources20
Helixi471 – 482Combined sources12
Helixi484 – 491Combined sources8
Beta strandi492 – 494Combined sources3
Helixi495 – 516Combined sources22
Beta strandi520 – 522Combined sources3
Helixi527 – 535Combined sources9
Beta strandi551 – 557Combined sources7
Turni558 – 560Combined sources3
Beta strandi565 – 570Combined sources6
Beta strandi574 – 576Combined sources3
Turni577 – 580Combined sources4
Helixi581 – 584Combined sources4
Helixi590 – 601Combined sources12
Beta strandi603 – 611Combined sources9
Helixi617 – 619Combined sources3
Helixi628 – 632Combined sources5
Turni635 – 637Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UAAX-ray3.00A/B1-673[»]
ProteinModelPortaliP09980.
SMRiP09980.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09980.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 280UvrD-like helicase ATP-bindingUniRule annotationAdd BLAST280
Domaini281 – 562UvrD-like helicase C-terminalUniRule annotationAdd BLAST282

Sequence similaritiesi

Belongs to the helicase family. UvrD subfamily.UniRule annotation
Contains 1 uvrD-like helicase ATP-binding domain.UniRule annotation
Contains 1 uvrD-like helicase C-terminal domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4R. Bacteria.
COG0210. LUCA.
HOGENOMiHOG000033015.
InParanoidiP09980.
KOiK03656.
OMAiEKVLMQN.
PhylomeDBiP09980.

Family and domain databases

Gene3Di1.10.10.160. 1 hit.
3.40.50.300. 4 hits.
HAMAPiMF_01920. Helicase_Rep. 1 hit.
InterProiIPR013986. DExx_box_DNA_helicase_dom.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR005752. Helicase_Rep.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 1 hit.
PfamiPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01074. rep. 1 hit.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09980-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLNPGQQQA VEFVTGPCLV LAGAGSGKTR VITNKIAHLI RGCGYQARHI
60 70 80 90 100
AAVTFTNKAA REMKERVGQT LGRKEARGLM ISTFHTLGLD IIKREYAALG
110 120 130 140 150
MKANFSLFDD TDQLALLKEL TEGLIEDDKV LLQQLISTIS NWKNDLKTPS
160 170 180 190 200
QAAASAIGER DRIFAHCYGL YDAHLKACNV LDFDDLILLP TLLLQRNEEV
210 220 230 240 250
RKRWQNKIRY LLVDEYQDTN TSQYELVKLL VGSRARFTVV GDDDQSIYSW
260 270 280 290 300
RGARPQNLVL LSQDFPALKV IKLEQNYRSS GRILKAANIL IANNPHVFEK
310 320 330 340 350
RLFSELGYGA ELKVLSANNE EHEAERVTGE LIAHHFVNKT QYKDYAILYR
360 370 380 390 400
GNHQSRVFEK FLMQNRIPYK ISGGTSFFSR PEIKDLLAYL RVLTNPDDDS
410 420 430 440 450
AFLRIVNTPK REIGPATLKK LGEWAMTRNK SMFTASFDMG LSQTLSGRGY
460 470 480 490 500
EALTRFTHWL AEIQRLAERE PIAAVRDLIH GMDYESWLYE TSPSPKAAEM
510 520 530 540 550
RMKNVNQLFS WMTEMLEGSE LDEPMTLTQV VTRFTLRDMM ERGESEEELD
560 570 580 590 600
QVQLMTLHAS KGLEFPYVYM VGMEEGFLPH QSSIDEDNID EERRLAYVGI
610 620 630 640 650
TRAQKELTFT LCKERRQYGE LVRPEPSRFL LELPQDDLIW EQERKVVSAE
660 670
ERMQKGQSHL ANLKAMMAAK RGK
Length:673
Mass (Da):77,024
Last modified:October 11, 2004 - v3
Checksum:i3338B3399C668E3E
GO

Sequence cautioni

The sequence CAA28481 differs from that shown. Reason: Frameshift at positions 620 and 657.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73R → H in CAA28481 (PubMed:3029683).Curated1
Sequence conflicti76A → Q in CAA28481 (PubMed:3029683).Curated1
Sequence conflicti163 – 165IFA → LLL in CAA28481 (PubMed:3029683).Curated3
Sequence conflicti196R → A in AAA67579 (PubMed:1379743).Curated1
Sequence conflicti202 – 203KR → NG in CAA28481 (PubMed:3029683).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04794 Genomic DNA. Translation: CAA28481.1. Frameshift.
M87049 Genomic DNA. Translation: AAA67579.1.
U00096 Genomic DNA. Translation: AAT48209.1.
AP009048 Genomic DNA. Translation: BAE77520.1.
M11055 Genomic DNA. Translation: AAA24518.1.
PIRiE65181. HJECDR.
RefSeqiWP_001238899.1. NZ_LN832404.1.
YP_026251.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48209; AAT48209; b3778.
BAE77520; BAE77520; BAE77520.
GeneIDi948292.
KEGGiecj:JW5604.
eco:b3778.
PATRICi32123047. VBIEscCol129921_3893.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04794 Genomic DNA. Translation: CAA28481.1. Frameshift.
M87049 Genomic DNA. Translation: AAA67579.1.
U00096 Genomic DNA. Translation: AAT48209.1.
AP009048 Genomic DNA. Translation: BAE77520.1.
M11055 Genomic DNA. Translation: AAA24518.1.
PIRiE65181. HJECDR.
RefSeqiWP_001238899.1. NZ_LN832404.1.
YP_026251.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UAAX-ray3.00A/B1-673[»]
ProteinModelPortaliP09980.
SMRiP09980.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263317. 66 interactors.
DIPiDIP-10662N.
IntActiP09980. 4 interactors.
MINTiMINT-1286362.
STRINGi511145.b3778.

Proteomic databases

PaxDbiP09980.
PRIDEiP09980.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48209; AAT48209; b3778.
BAE77520; BAE77520; BAE77520.
GeneIDi948292.
KEGGiecj:JW5604.
eco:b3778.
PATRICi32123047. VBIEscCol129921_3893.

Organism-specific databases

EchoBASEiEB0830.
EcoGeneiEG10837. rep.

Phylogenomic databases

eggNOGiENOG4105C4R. Bacteria.
COG0210. LUCA.
HOGENOMiHOG000033015.
InParanoidiP09980.
KOiK03656.
OMAiEKVLMQN.
PhylomeDBiP09980.

Enzyme and pathway databases

BioCyciEcoCyc:EG10837-MONOMER.
ECOL316407:JW5604-MONOMER.
MetaCyc:EG10837-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP09980.
PROiP09980.

Family and domain databases

Gene3Di1.10.10.160. 1 hit.
3.40.50.300. 4 hits.
HAMAPiMF_01920. Helicase_Rep. 1 hit.
InterProiIPR013986. DExx_box_DNA_helicase_dom.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR005752. Helicase_Rep.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 1 hit.
PfamiPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01074. rep. 1 hit.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiREP_ECOLI
AccessioniPrimary (citable) accession number: P09980
Secondary accession number(s): Q2M886, Q6BF05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.